Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling
Galectins (Gals) have emerged as potent immunoregulatory molecules that control chronic inflammation through distinct mechanisms. Gal-8, a tandem-repeat type Gal with unique preference for α2,3-sialylated glycans, is ubiquitously expressed, but little is known about its role in T-cell differentiatio...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_08189641_v94_n2_p213_Sampson |
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todo:paper_08189641_v94_n2_p213_Sampson2023-10-03T15:39:59Z Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling Sampson, J.F. Suryawanshi, A. Chen, W.-S. Rabinovich, G.A. Panjwani, N. cytotoxic T lymphocyte antigen 4 galectin 8 gamma interferon interleukin 10 interleukin 17 interleukin 2 interleukin 2 receptor beta interleukin 4 Smad3 protein STAT5 protein transcription factor FOXP3 transforming growth factor beta transforming growth factor beta receptor 2 cytotoxic T lymphocyte antigen 4 galectin galectin-8, mouse interleukin 10 interleukin 2 interleukin 2 receptor transforming growth factor beta animal cell antiproliferative activity Article CD4+ T lymphocyte controlled study cytokine production in vitro study lymphocyte differentiation lymphocyte proliferation mouse nonhuman protein function regulatory T lymphocyte signal transduction animal C57BL mouse cell culture cell differentiation immunology lymphocyte activation metabolism regulatory T lymphocyte Animals Cell Differentiation Cells, Cultured CTLA-4 Antigen Galectins Interleukin-10 Interleukin-2 Lymphocyte Activation Mice Mice, Inbred C57BL Receptors, Interleukin-2 Signal Transduction T-Lymphocytes, Regulatory Transforming Growth Factor beta Galectins (Gals) have emerged as potent immunoregulatory molecules that control chronic inflammation through distinct mechanisms. Gal-8, a tandem-repeat type Gal with unique preference for α2,3-sialylated glycans, is ubiquitously expressed, but little is known about its role in T-cell differentiation. Here we report that Gal-8 promotes the polyclonal differentiation of primary mouse regulatory T (Treg) cells in vitro. We further show that Gal-8 also facilitates antigen-specific differentiation of Treg cells, and that Treg cells polarized in the presence of Gal-8 express cytotoxic T-lymphocyte antigen-4 and interleukin (IL)-10 at a higher frequency than control Treg cells, and efficiently inhibit proliferation of activated T-cells in vitro. Investigation of the mechanism by which Gal-8 promotes Treg conversion revealed that Gal-8 activates transforming growth factor-β signaling and promotes sustained IL-2R signaling. Taken together, these data suggest that Gal-8 promotes the differentiation of highly suppressive Treg cells, which has implications for the treatment of inflammatory and autoimmune diseases. © 2016 Australasian Society for Immunology Inc. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08189641_v94_n2_p213_Sampson |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cytotoxic T lymphocyte antigen 4 galectin 8 gamma interferon interleukin 10 interleukin 17 interleukin 2 interleukin 2 receptor beta interleukin 4 Smad3 protein STAT5 protein transcription factor FOXP3 transforming growth factor beta transforming growth factor beta receptor 2 cytotoxic T lymphocyte antigen 4 galectin galectin-8, mouse interleukin 10 interleukin 2 interleukin 2 receptor transforming growth factor beta animal cell antiproliferative activity Article CD4+ T lymphocyte controlled study cytokine production in vitro study lymphocyte differentiation lymphocyte proliferation mouse nonhuman protein function regulatory T lymphocyte signal transduction animal C57BL mouse cell culture cell differentiation immunology lymphocyte activation metabolism regulatory T lymphocyte Animals Cell Differentiation Cells, Cultured CTLA-4 Antigen Galectins Interleukin-10 Interleukin-2 Lymphocyte Activation Mice Mice, Inbred C57BL Receptors, Interleukin-2 Signal Transduction T-Lymphocytes, Regulatory Transforming Growth Factor beta |
| spellingShingle |
cytotoxic T lymphocyte antigen 4 galectin 8 gamma interferon interleukin 10 interleukin 17 interleukin 2 interleukin 2 receptor beta interleukin 4 Smad3 protein STAT5 protein transcription factor FOXP3 transforming growth factor beta transforming growth factor beta receptor 2 cytotoxic T lymphocyte antigen 4 galectin galectin-8, mouse interleukin 10 interleukin 2 interleukin 2 receptor transforming growth factor beta animal cell antiproliferative activity Article CD4+ T lymphocyte controlled study cytokine production in vitro study lymphocyte differentiation lymphocyte proliferation mouse nonhuman protein function regulatory T lymphocyte signal transduction animal C57BL mouse cell culture cell differentiation immunology lymphocyte activation metabolism regulatory T lymphocyte Animals Cell Differentiation Cells, Cultured CTLA-4 Antigen Galectins Interleukin-10 Interleukin-2 Lymphocyte Activation Mice Mice, Inbred C57BL Receptors, Interleukin-2 Signal Transduction T-Lymphocytes, Regulatory Transforming Growth Factor beta Sampson, J.F. Suryawanshi, A. Chen, W.-S. Rabinovich, G.A. Panjwani, N. Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling |
| topic_facet |
cytotoxic T lymphocyte antigen 4 galectin 8 gamma interferon interleukin 10 interleukin 17 interleukin 2 interleukin 2 receptor beta interleukin 4 Smad3 protein STAT5 protein transcription factor FOXP3 transforming growth factor beta transforming growth factor beta receptor 2 cytotoxic T lymphocyte antigen 4 galectin galectin-8, mouse interleukin 10 interleukin 2 interleukin 2 receptor transforming growth factor beta animal cell antiproliferative activity Article CD4+ T lymphocyte controlled study cytokine production in vitro study lymphocyte differentiation lymphocyte proliferation mouse nonhuman protein function regulatory T lymphocyte signal transduction animal C57BL mouse cell culture cell differentiation immunology lymphocyte activation metabolism regulatory T lymphocyte Animals Cell Differentiation Cells, Cultured CTLA-4 Antigen Galectins Interleukin-10 Interleukin-2 Lymphocyte Activation Mice Mice, Inbred C57BL Receptors, Interleukin-2 Signal Transduction T-Lymphocytes, Regulatory Transforming Growth Factor beta |
| description |
Galectins (Gals) have emerged as potent immunoregulatory molecules that control chronic inflammation through distinct mechanisms. Gal-8, a tandem-repeat type Gal with unique preference for α2,3-sialylated glycans, is ubiquitously expressed, but little is known about its role in T-cell differentiation. Here we report that Gal-8 promotes the polyclonal differentiation of primary mouse regulatory T (Treg) cells in vitro. We further show that Gal-8 also facilitates antigen-specific differentiation of Treg cells, and that Treg cells polarized in the presence of Gal-8 express cytotoxic T-lymphocyte antigen-4 and interleukin (IL)-10 at a higher frequency than control Treg cells, and efficiently inhibit proliferation of activated T-cells in vitro. Investigation of the mechanism by which Gal-8 promotes Treg conversion revealed that Gal-8 activates transforming growth factor-β signaling and promotes sustained IL-2R signaling. Taken together, these data suggest that Gal-8 promotes the differentiation of highly suppressive Treg cells, which has implications for the treatment of inflammatory and autoimmune diseases. © 2016 Australasian Society for Immunology Inc. |
| format |
JOUR |
| author |
Sampson, J.F. Suryawanshi, A. Chen, W.-S. Rabinovich, G.A. Panjwani, N. |
| author_facet |
Sampson, J.F. Suryawanshi, A. Chen, W.-S. Rabinovich, G.A. Panjwani, N. |
| author_sort |
Sampson, J.F. |
| title |
Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling |
| title_short |
Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling |
| title_full |
Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling |
| title_fullStr |
Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling |
| title_full_unstemmed |
Galectin-8 promotes regulatory T-cell differentiation by modulating IL-2 and TGFβ signaling |
| title_sort |
galectin-8 promotes regulatory t-cell differentiation by modulating il-2 and tgfβ signaling |
| url |
http://hdl.handle.net/20.500.12110/paper_08189641_v94_n2_p213_Sampson |
| work_keys_str_mv |
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1807315466314579968 |