Development of an experimental ovarian tumor: Immunocytochemical analysis
Objective: The aim of the present work was to study whether immunocytochemical parameters present in the normal ovary were altered after tumor development under high gonadotropin levels. Methods: Ovarian tumors (luteoma): castrated female rats had an ovary grafted into the spleen; tumors were left t...
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todo:paper_08044643_v147_n3_p387_Sorianello2023-10-03T15:39:56Z Development of an experimental ovarian tumor: Immunocytochemical analysis Sorianello, E. Fritz, S. Beyer, C. Hales, D.B. Mayerhofer, A. Libertun, C. Lux-Lantos, V. aromatase connexin 43 cycline gap junction protein gonadotropin inhibin messenger RNA protein subunit steroidogenic acute regulatory protein synaptosomal associated protein 25 analytical parameters animal cell animal experiment animal tissue antigen expression apoptosis article cancer graft cell proliferation controlled study endocrine cell experimental neoplasm female gonadotropin blood level granulosa cell hormonal carcinogenesis hypertrophy immunocytochemistry immunohistochemistry luteoma nick end labeling nonhuman Northern blotting nucleotide sequence ovary follicle cell ovary tumor priority journal protein expression reverse transcription polymerase chain reaction spleen steroidogenesis theca cell Objective: The aim of the present work was to study whether immunocytochemical parameters present in the normal ovary were altered after tumor development under high gonadotropin levels. Methods: Ovarian tumors (luteoma): castrated female rats had an ovary grafted into the spleen; tumors were left to develop for 1, 2, 3 or 7 months. The presence of apoptotic cells (TUNEL method) and the expression of proliferating cell nuclear antigen (PCNA), gap junction protein (Cx43), steroidogenic acute regulatory protein (StAR), aromatase and synaptosome-associated protein of 25kDa (SNAP-25) were determined by immunocytochemistry. Some of these findings were confirmed by RT-PCR (Cx43, StAR, SNAP-25). Inhibin subunit mRNAs were investigated by Northern blot. Results: PCNA staining of tumors was mainly found in granulosa cells of transforming follicles and was absent from luteinized follicles. A nearly complete absence of apoptosis was observed. Cx43 was mainly found in follicles, while it was very weakly expressed or absent in luteinized follicles. StAR protein expression, indicating active steroidogenesis, was demonstrated only in luteinized follicles and in thecal cells, but was absent from granulosa cells. Aromatase immunoreactivity was very intense in granulosa and also present in luteal cells. Membrane-associated and cytoplasmic SNAP-25 immunostaining was determined in patches of endocrine cells in the follicles, as well as in the luteinized follicles. The expression of mRNAs for Cx43, StAR and SNAP-25 (RT-PCR) and inhibin subunits (Northern blots) were confirmed in 1, 3- and 7-month-old tumors. Conclusions: These results indicated that luteoma most likely develop from unruptured follicles by hypertrophy and proliferation of follicular cells. Circulating gonadotropins seem to play a fundamental role in maintaining the expression of proteins typically expressed in normal ovary, while avoiding apoptosis in this tissue. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_08044643_v147_n3_p387_Sorianello |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
aromatase connexin 43 cycline gap junction protein gonadotropin inhibin messenger RNA protein subunit steroidogenic acute regulatory protein synaptosomal associated protein 25 analytical parameters animal cell animal experiment animal tissue antigen expression apoptosis article cancer graft cell proliferation controlled study endocrine cell experimental neoplasm female gonadotropin blood level granulosa cell hormonal carcinogenesis hypertrophy immunocytochemistry immunohistochemistry luteoma nick end labeling nonhuman Northern blotting nucleotide sequence ovary follicle cell ovary tumor priority journal protein expression reverse transcription polymerase chain reaction spleen steroidogenesis theca cell |
| spellingShingle |
aromatase connexin 43 cycline gap junction protein gonadotropin inhibin messenger RNA protein subunit steroidogenic acute regulatory protein synaptosomal associated protein 25 analytical parameters animal cell animal experiment animal tissue antigen expression apoptosis article cancer graft cell proliferation controlled study endocrine cell experimental neoplasm female gonadotropin blood level granulosa cell hormonal carcinogenesis hypertrophy immunocytochemistry immunohistochemistry luteoma nick end labeling nonhuman Northern blotting nucleotide sequence ovary follicle cell ovary tumor priority journal protein expression reverse transcription polymerase chain reaction spleen steroidogenesis theca cell Sorianello, E. Fritz, S. Beyer, C. Hales, D.B. Mayerhofer, A. Libertun, C. Lux-Lantos, V. Development of an experimental ovarian tumor: Immunocytochemical analysis |
| topic_facet |
aromatase connexin 43 cycline gap junction protein gonadotropin inhibin messenger RNA protein subunit steroidogenic acute regulatory protein synaptosomal associated protein 25 analytical parameters animal cell animal experiment animal tissue antigen expression apoptosis article cancer graft cell proliferation controlled study endocrine cell experimental neoplasm female gonadotropin blood level granulosa cell hormonal carcinogenesis hypertrophy immunocytochemistry immunohistochemistry luteoma nick end labeling nonhuman Northern blotting nucleotide sequence ovary follicle cell ovary tumor priority journal protein expression reverse transcription polymerase chain reaction spleen steroidogenesis theca cell |
| description |
Objective: The aim of the present work was to study whether immunocytochemical parameters present in the normal ovary were altered after tumor development under high gonadotropin levels. Methods: Ovarian tumors (luteoma): castrated female rats had an ovary grafted into the spleen; tumors were left to develop for 1, 2, 3 or 7 months. The presence of apoptotic cells (TUNEL method) and the expression of proliferating cell nuclear antigen (PCNA), gap junction protein (Cx43), steroidogenic acute regulatory protein (StAR), aromatase and synaptosome-associated protein of 25kDa (SNAP-25) were determined by immunocytochemistry. Some of these findings were confirmed by RT-PCR (Cx43, StAR, SNAP-25). Inhibin subunit mRNAs were investigated by Northern blot. Results: PCNA staining of tumors was mainly found in granulosa cells of transforming follicles and was absent from luteinized follicles. A nearly complete absence of apoptosis was observed. Cx43 was mainly found in follicles, while it was very weakly expressed or absent in luteinized follicles. StAR protein expression, indicating active steroidogenesis, was demonstrated only in luteinized follicles and in thecal cells, but was absent from granulosa cells. Aromatase immunoreactivity was very intense in granulosa and also present in luteal cells. Membrane-associated and cytoplasmic SNAP-25 immunostaining was determined in patches of endocrine cells in the follicles, as well as in the luteinized follicles. The expression of mRNAs for Cx43, StAR and SNAP-25 (RT-PCR) and inhibin subunits (Northern blots) were confirmed in 1, 3- and 7-month-old tumors. Conclusions: These results indicated that luteoma most likely develop from unruptured follicles by hypertrophy and proliferation of follicular cells. Circulating gonadotropins seem to play a fundamental role in maintaining the expression of proteins typically expressed in normal ovary, while avoiding apoptosis in this tissue. |
| format |
JOUR |
| author |
Sorianello, E. Fritz, S. Beyer, C. Hales, D.B. Mayerhofer, A. Libertun, C. Lux-Lantos, V. |
| author_facet |
Sorianello, E. Fritz, S. Beyer, C. Hales, D.B. Mayerhofer, A. Libertun, C. Lux-Lantos, V. |
| author_sort |
Sorianello, E. |
| title |
Development of an experimental ovarian tumor: Immunocytochemical analysis |
| title_short |
Development of an experimental ovarian tumor: Immunocytochemical analysis |
| title_full |
Development of an experimental ovarian tumor: Immunocytochemical analysis |
| title_fullStr |
Development of an experimental ovarian tumor: Immunocytochemical analysis |
| title_full_unstemmed |
Development of an experimental ovarian tumor: Immunocytochemical analysis |
| title_sort |
development of an experimental ovarian tumor: immunocytochemical analysis |
| url |
http://hdl.handle.net/20.500.12110/paper_08044643_v147_n3_p387_Sorianello |
| work_keys_str_mv |
AT sorianelloe developmentofanexperimentalovariantumorimmunocytochemicalanalysis AT fritzs developmentofanexperimentalovariantumorimmunocytochemicalanalysis AT beyerc developmentofanexperimentalovariantumorimmunocytochemicalanalysis AT halesdb developmentofanexperimentalovariantumorimmunocytochemicalanalysis AT mayerhofera developmentofanexperimentalovariantumorimmunocytochemicalanalysis AT libertunc developmentofanexperimentalovariantumorimmunocytochemicalanalysis AT luxlantosv developmentofanexperimentalovariantumorimmunocytochemicalanalysis |
| _version_ |
1807319032005656576 |