Induction of ovarian growth in the red claw crayfish, cherax quadricarinatus, by the enkephalinergic antagonist naloxone: In vivo and in vitro studies

The effects of spiperone and domperidone (dopaminergic antagonists), as well as naloxone (enkephalinergic antagonist) on ovarian growth of the crayfish Cherax quadricarinatus, were evaluated during the non-reproductive period. In vivo assays were carried out by administering these compounds by addin...

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Autores principales: Cahansky, A.V., Medesani, D.A., RodrÍguez, E.M.
Formato: JOUR
Materias:
Cherax
Crustaceans
Dopaminergic
Enkephalinergic
Ovarian growth
Astacoidea
Cherax quadricarinatus
Crustacea
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_07924259_v51_n2_p61_Cahansky
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_07924259_v51_n2_p61_Cahansky
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spelling todo:paper_07924259_v51_n2_p61_Cahansky2023-10-03T15:39:53Z Induction of ovarian growth in the red claw crayfish, cherax quadricarinatus, by the enkephalinergic antagonist naloxone: In vivo and in vitro studies Cahansky, A.V. Medesani, D.A. RodrÍguez, E.M. Cherax Crustaceans Dopaminergic Enkephalinergic Ovarian growth Astacoidea Cherax Cherax quadricarinatus Crustacea The effects of spiperone and domperidone (dopaminergic antagonists), as well as naloxone (enkephalinergic antagonist) on ovarian growth of the crayfish Cherax quadricarinatus, were evaluated during the non-reproductive period. In vivo assays were carried out by administering these compounds by adding them to the crayfish food, and subsequently measuring the oocyte diameter (oocytes in secondary vitellogenesis). Only naloxone produced a significantly (p <0.05) higher oocyte diameter than that of the controls, suggesting that this antagonist was blocking the effects of endogenous enkcephalins on the secretion of one or more neurohormones involved in crustacean reproduction. To test this hypothesis, an in vitro incubation of ovarian pieces with thoracic ganglion (TG), a source of the gonad stimulating hormone (GSH), or eyestalk tissue (ET), the source of the gonad inhibiting hormone (GIH), with or without naloxone added to the incubation medium, was done. A significant increase of ovarian growth was observed when TG was present. Moreover, the addition of naloxone to this preparation was able to further increase the ovarian growth, presumably by preventing enkephalinergic inhibition of GSH secretion. © 2008 Taylor & Francis Group, LLC. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07924259_v51_n2_p61_Cahansky
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cherax
Crustaceans
Dopaminergic
Enkephalinergic
Ovarian growth
Astacoidea
Cherax
Cherax quadricarinatus
Crustacea
spellingShingle Cherax
Crustaceans
Dopaminergic
Enkephalinergic
Ovarian growth
Astacoidea
Cherax
Cherax quadricarinatus
Crustacea
Cahansky, A.V.
Medesani, D.A.
RodrÍguez, E.M.
Induction of ovarian growth in the red claw crayfish, cherax quadricarinatus, by the enkephalinergic antagonist naloxone: In vivo and in vitro studies
topic_facet Cherax
Crustaceans
Dopaminergic
Enkephalinergic
Ovarian growth
Astacoidea
Cherax
Cherax quadricarinatus
Crustacea
description The effects of spiperone and domperidone (dopaminergic antagonists), as well as naloxone (enkephalinergic antagonist) on ovarian growth of the crayfish Cherax quadricarinatus, were evaluated during the non-reproductive period. In vivo assays were carried out by administering these compounds by adding them to the crayfish food, and subsequently measuring the oocyte diameter (oocytes in secondary vitellogenesis). Only naloxone produced a significantly (p <0.05) higher oocyte diameter than that of the controls, suggesting that this antagonist was blocking the effects of endogenous enkcephalins on the secretion of one or more neurohormones involved in crustacean reproduction. To test this hypothesis, an in vitro incubation of ovarian pieces with thoracic ganglion (TG), a source of the gonad stimulating hormone (GSH), or eyestalk tissue (ET), the source of the gonad inhibiting hormone (GIH), with or without naloxone added to the incubation medium, was done. A significant increase of ovarian growth was observed when TG was present. Moreover, the addition of naloxone to this preparation was able to further increase the ovarian growth, presumably by preventing enkephalinergic inhibition of GSH secretion. © 2008 Taylor & Francis Group, LLC.
format JOUR
author Cahansky, A.V.
Medesani, D.A.
RodrÍguez, E.M.
author_facet Cahansky, A.V.
Medesani, D.A.
RodrÍguez, E.M.
author_sort Cahansky, A.V.
title Induction of ovarian growth in the red claw crayfish, cherax quadricarinatus, by the enkephalinergic antagonist naloxone: In vivo and in vitro studies
title_short Induction of ovarian growth in the red claw crayfish, cherax quadricarinatus, by the enkephalinergic antagonist naloxone: In vivo and in vitro studies
title_full Induction of ovarian growth in the red claw crayfish, cherax quadricarinatus, by the enkephalinergic antagonist naloxone: In vivo and in vitro studies
title_fullStr Induction of ovarian growth in the red claw crayfish, cherax quadricarinatus, by the enkephalinergic antagonist naloxone: In vivo and in vitro studies
title_full_unstemmed Induction of ovarian growth in the red claw crayfish, cherax quadricarinatus, by the enkephalinergic antagonist naloxone: In vivo and in vitro studies
title_sort induction of ovarian growth in the red claw crayfish, cherax quadricarinatus, by the enkephalinergic antagonist naloxone: in vivo and in vitro studies
url http://hdl.handle.net/20.500.12110/paper_07924259_v51_n2_p61_Cahansky
work_keys_str_mv AT cahanskyav inductionofovariangrowthintheredclawcrayfishcheraxquadricarinatusbytheenkephalinergicantagonistnaloxoneinvivoandinvitrostudies
AT medesanida inductionofovariangrowthintheredclawcrayfishcheraxquadricarinatusbytheenkephalinergicantagonistnaloxoneinvivoandinvitrostudies
AT rodriguezem inductionofovariangrowthintheredclawcrayfishcheraxquadricarinatusbytheenkephalinergicantagonistnaloxoneinvivoandinvitrostudies
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