Expression and maintenance of plasmid resistance in regenerating protoplasts of Bacillus subtilis
Expression of plasmid-encoded resistances in regenerating protoplasts of Bacillus subtilis occurs only after wall synthesis has been resumed. This is observed for protoplasts obtained from cells already containing plasmids (pC194, pT127, pK545) or for plasmid-bearing cells coming from a PEG-mediated...
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todo:paper_07692609_v139_n4_p403_SanchezRivas2023-10-03T15:39:41Z Expression and maintenance of plasmid resistance in regenerating protoplasts of Bacillus subtilis Sanchez-Rivas, C. Bacillus subtilis, Plasmid, Protoplast Transformation, Gene expression, Cell wall regeneration bacillus subtilis bacterium transformation cell wall gene expression genetic engineering nonhuman plasmid priority journal protoplast Bacillus subtilis Cell Wall Culture Media Drug Resistance, Microbial Gene Expression Regulation Osmotic Pressure Protoplasts R Factors Support, Non-U.S. Gov't Transformation, Bacterial Expression of plasmid-encoded resistances in regenerating protoplasts of Bacillus subtilis occurs only after wall synthesis has been resumed. This is observed for protoplasts obtained from cells already containing plasmids (pC194, pT127, pK545) or for plasmid-bearing cells coming from a PEG-mediated transformation. Recovery of expression needs a 2-h incubation of protoplasts, previously washed to get rid of their lysozyme content, in rich hypertonic medium (SMMP). A longer incubation (24-h) results in the obtention of regenerants; however, most of them have lost their resistant phenotype in contrast to those obtained from the usual solid regeneration plates. This finding suggests either a high curing effect or some kind of gene inactivation phenomenon. Discussion is focused on the critical points that have to be considered when polyethylenglycol-mediated transformation of protoplasts is applied to recombinant DNA technology. © 1988. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07692609_v139_n4_p403_SanchezRivas |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bacillus subtilis, Plasmid, Protoplast Transformation, Gene expression, Cell wall regeneration bacillus subtilis bacterium transformation cell wall gene expression genetic engineering nonhuman plasmid priority journal protoplast Bacillus subtilis Cell Wall Culture Media Drug Resistance, Microbial Gene Expression Regulation Osmotic Pressure Protoplasts R Factors Support, Non-U.S. Gov't Transformation, Bacterial |
| spellingShingle |
Bacillus subtilis, Plasmid, Protoplast Transformation, Gene expression, Cell wall regeneration bacillus subtilis bacterium transformation cell wall gene expression genetic engineering nonhuman plasmid priority journal protoplast Bacillus subtilis Cell Wall Culture Media Drug Resistance, Microbial Gene Expression Regulation Osmotic Pressure Protoplasts R Factors Support, Non-U.S. Gov't Transformation, Bacterial Sanchez-Rivas, C. Expression and maintenance of plasmid resistance in regenerating protoplasts of Bacillus subtilis |
| topic_facet |
Bacillus subtilis, Plasmid, Protoplast Transformation, Gene expression, Cell wall regeneration bacillus subtilis bacterium transformation cell wall gene expression genetic engineering nonhuman plasmid priority journal protoplast Bacillus subtilis Cell Wall Culture Media Drug Resistance, Microbial Gene Expression Regulation Osmotic Pressure Protoplasts R Factors Support, Non-U.S. Gov't Transformation, Bacterial |
| description |
Expression of plasmid-encoded resistances in regenerating protoplasts of Bacillus subtilis occurs only after wall synthesis has been resumed. This is observed for protoplasts obtained from cells already containing plasmids (pC194, pT127, pK545) or for plasmid-bearing cells coming from a PEG-mediated transformation. Recovery of expression needs a 2-h incubation of protoplasts, previously washed to get rid of their lysozyme content, in rich hypertonic medium (SMMP). A longer incubation (24-h) results in the obtention of regenerants; however, most of them have lost their resistant phenotype in contrast to those obtained from the usual solid regeneration plates. This finding suggests either a high curing effect or some kind of gene inactivation phenomenon. Discussion is focused on the critical points that have to be considered when polyethylenglycol-mediated transformation of protoplasts is applied to recombinant DNA technology. © 1988. |
| format |
JOUR |
| author |
Sanchez-Rivas, C. |
| author_facet |
Sanchez-Rivas, C. |
| author_sort |
Sanchez-Rivas, C. |
| title |
Expression and maintenance of plasmid resistance in regenerating protoplasts of Bacillus subtilis |
| title_short |
Expression and maintenance of plasmid resistance in regenerating protoplasts of Bacillus subtilis |
| title_full |
Expression and maintenance of plasmid resistance in regenerating protoplasts of Bacillus subtilis |
| title_fullStr |
Expression and maintenance of plasmid resistance in regenerating protoplasts of Bacillus subtilis |
| title_full_unstemmed |
Expression and maintenance of plasmid resistance in regenerating protoplasts of Bacillus subtilis |
| title_sort |
expression and maintenance of plasmid resistance in regenerating protoplasts of bacillus subtilis |
| url |
http://hdl.handle.net/20.500.12110/paper_07692609_v139_n4_p403_SanchezRivas |
| work_keys_str_mv |
AT sanchezrivasc expressionandmaintenanceofplasmidresistanceinregeneratingprotoplastsofbacillussubtilis |
| _version_ |
1807316778039115776 |