One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns

A relevant question in cell biology with broad implications in biomedicine is how the organization and dynamics of interacting membranes modulate signaling cascades that involve cell-cell contact. The functionalization of surfaces with supported lipid bilayers containing tethered proteins is a parti...

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Detalles Bibliográficos
Autores principales:	Sánchez, M.F., Dodes Traian, M.M., Levi, V., Carrer, D.C.
Formato:	JOUR
Materias:	Cell signaling Cells Cytology Electron device manufacture Fluorescence Fluorescence spectroscopy Photobleaching Photons Proteins Spectroscopic analysis Direct interactions Fluorescence Correlation Spectroscopy Fluorescence recovery after photobleaching Functionalizations Laser scanning microscope Microcontact printing techniques Signaling cascades Supported lipid bilayers Lipid bilayers ligand lipid bilayer chemistry photon spectrofluorometry Ligands Lipid Bilayers Spectrometry, Fluorescence
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_07437463_v31_n43_p11943_Sanchez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_07437463_v31_n43_p11943_Sanchez
record_format	dspace
spelling	todo:paper_07437463_v31_n43_p11943_Sanchez2023-10-03T15:38:48Z One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns Sánchez, M.F. Dodes Traian, M.M. Levi, V. Carrer, D.C. Cell signaling Cells Cytology Electron device manufacture Fluorescence Fluorescence spectroscopy Photobleaching Photons Proteins Spectroscopic analysis Direct interactions Fluorescence Correlation Spectroscopy Fluorescence recovery after photobleaching Functionalizations Laser scanning microscope Microcontact printing techniques Signaling cascades Supported lipid bilayers Lipid bilayers ligand lipid bilayer chemistry lipid bilayer photon spectrofluorometry Ligands Lipid Bilayers Photons Spectrometry, Fluorescence A relevant question in cell biology with broad implications in biomedicine is how the organization and dynamics of interacting membranes modulate signaling cascades that involve cell-cell contact. The functionalization of surfaces with supported lipid bilayers containing tethered proteins is a particularly useful method to present ligands with membrane-like mobility to cells. Here, we present a method to generate micrometer-sized patches of lipid bilayers decorated with proteins. The method uses an economic microcontact printing technique based on one-photon lithography that can be easily implemented in a commercial laser scanning microscope. We verified that both proteins and lipids freely diffuse within the patterned bilayer, as assessed by z-scan fluorescence correlation spectroscopy and fluorescence recovery after photobleaching. These results suggest that the supported lipid bilayer patterns constitute an optimal system to explore processes involving direct interactions between cells. We also illustrate possible applications of this method by exploring the interaction of cells expressing the Fas receptor and patterns of lipid bilayers containing an agonist antibody against Fas. © 2015 American Chemical Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07437463_v31_n43_p11943_Sanchez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Cell signaling Cells Cytology Electron device manufacture Fluorescence Fluorescence spectroscopy Photobleaching Photons Proteins Spectroscopic analysis Direct interactions Fluorescence Correlation Spectroscopy Fluorescence recovery after photobleaching Functionalizations Laser scanning microscope Microcontact printing techniques Signaling cascades Supported lipid bilayers Lipid bilayers ligand lipid bilayer chemistry lipid bilayer photon spectrofluorometry Ligands Lipid Bilayers Photons Spectrometry, Fluorescence
spellingShingle	Cell signaling Cells Cytology Electron device manufacture Fluorescence Fluorescence spectroscopy Photobleaching Photons Proteins Spectroscopic analysis Direct interactions Fluorescence Correlation Spectroscopy Fluorescence recovery after photobleaching Functionalizations Laser scanning microscope Microcontact printing techniques Signaling cascades Supported lipid bilayers Lipid bilayers ligand lipid bilayer chemistry lipid bilayer photon spectrofluorometry Ligands Lipid Bilayers Photons Spectrometry, Fluorescence Sánchez, M.F. Dodes Traian, M.M. Levi, V. Carrer, D.C. One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns
topic_facet	Cell signaling Cells Cytology Electron device manufacture Fluorescence Fluorescence spectroscopy Photobleaching Photons Proteins Spectroscopic analysis Direct interactions Fluorescence Correlation Spectroscopy Fluorescence recovery after photobleaching Functionalizations Laser scanning microscope Microcontact printing techniques Signaling cascades Supported lipid bilayers Lipid bilayers ligand lipid bilayer chemistry lipid bilayer photon spectrofluorometry Ligands Lipid Bilayers Photons Spectrometry, Fluorescence
description	A relevant question in cell biology with broad implications in biomedicine is how the organization and dynamics of interacting membranes modulate signaling cascades that involve cell-cell contact. The functionalization of surfaces with supported lipid bilayers containing tethered proteins is a particularly useful method to present ligands with membrane-like mobility to cells. Here, we present a method to generate micrometer-sized patches of lipid bilayers decorated with proteins. The method uses an economic microcontact printing technique based on one-photon lithography that can be easily implemented in a commercial laser scanning microscope. We verified that both proteins and lipids freely diffuse within the patterned bilayer, as assessed by z-scan fluorescence correlation spectroscopy and fluorescence recovery after photobleaching. These results suggest that the supported lipid bilayer patterns constitute an optimal system to explore processes involving direct interactions between cells. We also illustrate possible applications of this method by exploring the interaction of cells expressing the Fas receptor and patterns of lipid bilayers containing an agonist antibody against Fas. © 2015 American Chemical Society.
format	JOUR
author	Sánchez, M.F. Dodes Traian, M.M. Levi, V. Carrer, D.C.
author_facet	Sánchez, M.F. Dodes Traian, M.M. Levi, V. Carrer, D.C.
author_sort	Sánchez, M.F.
title	One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns
title_short	One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns
title_full	One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns
title_fullStr	One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns
title_full_unstemmed	One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns
title_sort	one-photon lithography for high-quality lipid bilayer micropatterns
url	http://hdl.handle.net/20.500.12110/paper_07437463_v31_n43_p11943_Sanchez
work_keys_str_mv	AT sanchezmf onephotonlithographyforhighqualitylipidbilayermicropatterns AT dodestraianmm onephotonlithographyforhighqualitylipidbilayermicropatterns AT leviv onephotonlithographyforhighqualitylipidbilayermicropatterns AT carrerdc onephotonlithographyforhighqualitylipidbilayermicropatterns
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One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns

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