Corticosteroid synthesis in the central nervous system

The possibility that adrenocorticosteroids might be synthesized in the central nervous system was assessed by RT-PCR using primers for the CYP11B1 gene which codes for 11β-hydroxylase, the enzyme responsible for corticosterone and cortisol formation in the zona fasciculata, incubation of minces of s...

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Detalles Bibliográficos
Autores principales:	Gomez-Sanchez, C.E., Zhou, M.Y., Cozza, E.N., Morita, H., Eddleman, F.C., Gomez-Sanchez, E.P.
Formato:	JOUR
Materias:	19 ethynyldeoxycorticosterone corticosterone dehydrocorticosterone steroid 11beta monooxygenase steroid 11beta monooxygenase inhibitor unclassified drug animal cell animal tissue blood pressure measurement central nervous system function conference paper dose response enzyme mechanism hypertension inhibition kinetics intracerebroventricular drug administration male nonhuman priority journal rat steroidogenesis tissue distribution
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_07435800_v22_n4_p463_GomezSanchez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_07435800_v22_n4_p463_GomezSanchez
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spelling	todo:paper_07435800_v22_n4_p463_GomezSanchez2023-10-03T15:38:28Z Corticosteroid synthesis in the central nervous system Gomez-Sanchez, C.E. Zhou, M.Y. Cozza, E.N. Morita, H. Eddleman, F.C. Gomez-Sanchez, E.P. 19 ethynyldeoxycorticosterone corticosterone dehydrocorticosterone steroid 11beta monooxygenase steroid 11beta monooxygenase inhibitor unclassified drug animal cell animal tissue blood pressure measurement central nervous system function conference paper dose response enzyme mechanism hypertension inhibition kinetics intracerebroventricular drug administration male nonhuman priority journal rat steroidogenesis tissue distribution The possibility that adrenocorticosteroids might be synthesized in the central nervous system was assessed by RT-PCR using primers for the CYP11B1 gene which codes for 11β-hydroxylase, the enzyme responsible for corticosterone and cortisol formation in the zona fasciculata, incubation of minces of several areas of the brain with 3H-DOC and measuring steroid metabolites, and determining the effect of the intracerebroventricular infusion of the 11β-hydroxylase mechanism-based inhibitor 19- ethynyldeoxycorticosterone upon the salt-induced increase in blood pressure in SS/jr rats. Significant, though small relative to the adrenal, amounts of mRNA for 11β-hydroxylase was found in the aorta, cerebrum, cerebellum, hippocampus, hypothalamus and amygdala, but not in the heart. Brain minces converted 3H-DOC to corticosterone and 11-dehydrocorticosterone to a greater degree than to 18-OH-DOC. The effect of 19- ethynyldeoxycorticosterone was dose dependent, with the lower doses preventing salt-induced hypertension and the higher doses having no effect or increasing the blood pressure. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07435800_v22_n4_p463_GomezSanchez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	19 ethynyldeoxycorticosterone corticosterone dehydrocorticosterone steroid 11beta monooxygenase steroid 11beta monooxygenase inhibitor unclassified drug animal cell animal tissue blood pressure measurement central nervous system function conference paper dose response enzyme mechanism hypertension inhibition kinetics intracerebroventricular drug administration male nonhuman priority journal rat steroidogenesis tissue distribution
spellingShingle	19 ethynyldeoxycorticosterone corticosterone dehydrocorticosterone steroid 11beta monooxygenase steroid 11beta monooxygenase inhibitor unclassified drug animal cell animal tissue blood pressure measurement central nervous system function conference paper dose response enzyme mechanism hypertension inhibition kinetics intracerebroventricular drug administration male nonhuman priority journal rat steroidogenesis tissue distribution Gomez-Sanchez, C.E. Zhou, M.Y. Cozza, E.N. Morita, H. Eddleman, F.C. Gomez-Sanchez, E.P. Corticosteroid synthesis in the central nervous system
topic_facet	19 ethynyldeoxycorticosterone corticosterone dehydrocorticosterone steroid 11beta monooxygenase steroid 11beta monooxygenase inhibitor unclassified drug animal cell animal tissue blood pressure measurement central nervous system function conference paper dose response enzyme mechanism hypertension inhibition kinetics intracerebroventricular drug administration male nonhuman priority journal rat steroidogenesis tissue distribution
description	The possibility that adrenocorticosteroids might be synthesized in the central nervous system was assessed by RT-PCR using primers for the CYP11B1 gene which codes for 11β-hydroxylase, the enzyme responsible for corticosterone and cortisol formation in the zona fasciculata, incubation of minces of several areas of the brain with 3H-DOC and measuring steroid metabolites, and determining the effect of the intracerebroventricular infusion of the 11β-hydroxylase mechanism-based inhibitor 19- ethynyldeoxycorticosterone upon the salt-induced increase in blood pressure in SS/jr rats. Significant, though small relative to the adrenal, amounts of mRNA for 11β-hydroxylase was found in the aorta, cerebrum, cerebellum, hippocampus, hypothalamus and amygdala, but not in the heart. Brain minces converted 3H-DOC to corticosterone and 11-dehydrocorticosterone to a greater degree than to 18-OH-DOC. The effect of 19- ethynyldeoxycorticosterone was dose dependent, with the lower doses preventing salt-induced hypertension and the higher doses having no effect or increasing the blood pressure.
format	JOUR
author	Gomez-Sanchez, C.E. Zhou, M.Y. Cozza, E.N. Morita, H. Eddleman, F.C. Gomez-Sanchez, E.P.
author_facet	Gomez-Sanchez, C.E. Zhou, M.Y. Cozza, E.N. Morita, H. Eddleman, F.C. Gomez-Sanchez, E.P.
author_sort	Gomez-Sanchez, C.E.
title	Corticosteroid synthesis in the central nervous system
title_short	Corticosteroid synthesis in the central nervous system
title_full	Corticosteroid synthesis in the central nervous system
title_fullStr	Corticosteroid synthesis in the central nervous system
title_full_unstemmed	Corticosteroid synthesis in the central nervous system
title_sort	corticosteroid synthesis in the central nervous system
url	http://hdl.handle.net/20.500.12110/paper_07435800_v22_n4_p463_GomezSanchez
work_keys_str_mv	AT gomezsanchezce corticosteroidsynthesisinthecentralnervoussystem AT zhoumy corticosteroidsynthesisinthecentralnervoussystem AT cozzaen corticosteroidsynthesisinthecentralnervoussystem AT moritah corticosteroidsynthesisinthecentralnervoussystem AT eddlemanfc corticosteroidsynthesisinthecentralnervoussystem AT gomezsanchezep corticosteroidsynthesisinthecentralnervoussystem
_version_	1807324246115876864

Corticosteroid synthesis in the central nervous system

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