Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice
Since genetic damage induced by ethanol exposure is controversial and incomplete and because germ and somatic cells constitute bioindicators for monitoring reproductive toxicity and genotoxic actions of ethanol consumption, the purpose of the present investigation was to evaluate morphological sperm...
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todo:paper_07422091_v27_n4_p237_Cebral2023-10-03T15:38:22Z Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice Cebral, E. Abrevaya, X.C. Mudry, M.D. CF-1 mice Ethanol Micronucleus Oocyte Spermatozoa alcohol adult animal alcohol blood level alcohol consumption animal cell animal experiment animal model article cell activation cell damage cell nucleus cell structure CF1 mouse conception controlled study cytotoxicity female gamete genotoxicity gestation period male micronucleus mouse mouse strain nonhuman oocyte priority journal reproductive toxicity sperm Alcohol Drinking Animals Ethanol Female Male Mice Mice, Inbred Strains Micronuclei, Chromosome-Defective Mutagenicity Tests Oocytes Pregnancy Reproduction Spermatozoa Mus Since genetic damage induced by ethanol exposure is controversial and incomplete and because germ and somatic cells constitute bioindicators for monitoring reproductive toxicity and genotoxic actions of ethanol consumption, the purpose of the present investigation was to evaluate morphological sperm, oocyte alterations and parental genotoxic effects after sub-chronic ethanol intake in the CF-1 outbred mouse strain. Ethanol 10% was administered to CF-1 adult male (treated males, TM) and female (treated females, TF) mice for 27 days, whereas water was given to controls from both sexes too (CM and CF). Post-treatment micronucleus frequency (MN-PCE/1,000/mouse) and gamete morphology were evaluated. To test whether change of female reproductive status results in maternal genotoxicity, CF-1 females received ethanol 10% (exposed group, periconceptionally treated females (PTF)) or water (control group, pregnant control females (PCF)) in drinking water for 17 days previous and up to 10 days of gestation. TM had a high percentage of abnormal spermatozoa vs CM (p < 0.001) and elevated parthenogenetic activated oocyte frequency appeared in TF vs CF (p < 0.001). Sub-chronic ethanol ingestion induced increased MN frequency in TM and TF (p < 0.01). In PTF, where blood alcohol concentrations were between 19-28 mg/dl, very significantly increased MN frequency was found vs PCF (p < 0.01), whereas MN values were similar to TF. These results show that sub-chronic alcohol ingestion in CF-1 mice produces sperm head dysmorphogenesis and oocyte nuclear anomalies, suggesting that morphological abnormalities in germ cells are probably related to parental genotoxicity after ethanol consumption. © 2011 Springer Science+Business Media B.V. Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Abrevaya, X.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07422091_v27_n4_p237_Cebral |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CF-1 mice Ethanol Micronucleus Oocyte Spermatozoa alcohol adult animal alcohol blood level alcohol consumption animal cell animal experiment animal model article cell activation cell damage cell nucleus cell structure CF1 mouse conception controlled study cytotoxicity female gamete genotoxicity gestation period male micronucleus mouse mouse strain nonhuman oocyte priority journal reproductive toxicity sperm Alcohol Drinking Animals Ethanol Female Male Mice Mice, Inbred Strains Micronuclei, Chromosome-Defective Mutagenicity Tests Oocytes Pregnancy Reproduction Spermatozoa Mus |
| spellingShingle |
CF-1 mice Ethanol Micronucleus Oocyte Spermatozoa alcohol adult animal alcohol blood level alcohol consumption animal cell animal experiment animal model article cell activation cell damage cell nucleus cell structure CF1 mouse conception controlled study cytotoxicity female gamete genotoxicity gestation period male micronucleus mouse mouse strain nonhuman oocyte priority journal reproductive toxicity sperm Alcohol Drinking Animals Ethanol Female Male Mice Mice, Inbred Strains Micronuclei, Chromosome-Defective Mutagenicity Tests Oocytes Pregnancy Reproduction Spermatozoa Mus Cebral, E. Abrevaya, X.C. Mudry, M.D. Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice |
| topic_facet |
CF-1 mice Ethanol Micronucleus Oocyte Spermatozoa alcohol adult animal alcohol blood level alcohol consumption animal cell animal experiment animal model article cell activation cell damage cell nucleus cell structure CF1 mouse conception controlled study cytotoxicity female gamete genotoxicity gestation period male micronucleus mouse mouse strain nonhuman oocyte priority journal reproductive toxicity sperm Alcohol Drinking Animals Ethanol Female Male Mice Mice, Inbred Strains Micronuclei, Chromosome-Defective Mutagenicity Tests Oocytes Pregnancy Reproduction Spermatozoa Mus |
| description |
Since genetic damage induced by ethanol exposure is controversial and incomplete and because germ and somatic cells constitute bioindicators for monitoring reproductive toxicity and genotoxic actions of ethanol consumption, the purpose of the present investigation was to evaluate morphological sperm, oocyte alterations and parental genotoxic effects after sub-chronic ethanol intake in the CF-1 outbred mouse strain. Ethanol 10% was administered to CF-1 adult male (treated males, TM) and female (treated females, TF) mice for 27 days, whereas water was given to controls from both sexes too (CM and CF). Post-treatment micronucleus frequency (MN-PCE/1,000/mouse) and gamete morphology were evaluated. To test whether change of female reproductive status results in maternal genotoxicity, CF-1 females received ethanol 10% (exposed group, periconceptionally treated females (PTF)) or water (control group, pregnant control females (PCF)) in drinking water for 17 days previous and up to 10 days of gestation. TM had a high percentage of abnormal spermatozoa vs CM (p < 0.001) and elevated parthenogenetic activated oocyte frequency appeared in TF vs CF (p < 0.001). Sub-chronic ethanol ingestion induced increased MN frequency in TM and TF (p < 0.01). In PTF, where blood alcohol concentrations were between 19-28 mg/dl, very significantly increased MN frequency was found vs PCF (p < 0.01), whereas MN values were similar to TF. These results show that sub-chronic alcohol ingestion in CF-1 mice produces sperm head dysmorphogenesis and oocyte nuclear anomalies, suggesting that morphological abnormalities in germ cells are probably related to parental genotoxicity after ethanol consumption. © 2011 Springer Science+Business Media B.V. |
| format |
JOUR |
| author |
Cebral, E. Abrevaya, X.C. Mudry, M.D. |
| author_facet |
Cebral, E. Abrevaya, X.C. Mudry, M.D. |
| author_sort |
Cebral, E. |
| title |
Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice |
| title_short |
Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice |
| title_full |
Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice |
| title_fullStr |
Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice |
| title_full_unstemmed |
Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice |
| title_sort |
male and female reproductive toxicity induced by sub-chronic ethanol exposure in cf-1 mice |
| url |
http://hdl.handle.net/20.500.12110/paper_07422091_v27_n4_p237_Cebral |
| work_keys_str_mv |
AT cebrale maleandfemalereproductivetoxicityinducedbysubchronicethanolexposureincf1mice AT abrevayaxc maleandfemalereproductivetoxicityinducedbysubchronicethanolexposureincf1mice AT mudrymd maleandfemalereproductivetoxicityinducedbysubchronicethanolexposureincf1mice |
| _version_ |
1807324418663251968 |