Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy

The aim of this study was to analyze the effectiveness of UV-C light (0–10.6 kJ/m2) assisted by mild heat treatment (50 °C) on the inactivation of Saccharomyces cerevisiae KE 162 in peptone water and fresh carrot-orange juice blend (pH: 3.8; 9.8°Brix; 707 NTU; absorption coefficient: 0.17 cm−1). Yea...

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Autores principales:	García Carrillo, M., Ferrario, M., Guerrero, S.
Formato:	JOUR
Materias:	Flow cytometry Mild heat Transmission electron microscopy UV-C light carrot chemistry evaluation study flow cytometry food preservation fruit and vegetable juice growth, development and aging heat microbial viability microbiology procedures radiation response Saccharomyces cerevisiae sweet orange transmission electron microscopy ultrastructure ultraviolet radiation Citrus sinensis Daucus carota Flow Cytometry Food Preservation Fruit and Vegetable Juices Hot Temperature Microbial Viability Microscopy, Electron, Transmission Ultraviolet Rays
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_07400020_v73_n_p1_GarciaCarrillo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_07400020_v73_n_p1_GarciaCarrillo
record_format	dspace
spelling	todo:paper_07400020_v73_n_p1_GarciaCarrillo2023-10-03T15:38:07Z Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy García Carrillo, M. Ferrario, M. Guerrero, S. Flow cytometry Mild heat Transmission electron microscopy UV-C light carrot chemistry evaluation study flow cytometry food preservation fruit and vegetable juice growth, development and aging heat microbial viability microbiology procedures radiation response Saccharomyces cerevisiae sweet orange transmission electron microscopy ultrastructure ultraviolet radiation Citrus sinensis Daucus carota Flow Cytometry Food Preservation Fruit and Vegetable Juices Hot Temperature Microbial Viability Microscopy, Electron, Transmission Saccharomyces cerevisiae Ultraviolet Rays The aim of this study was to analyze the effectiveness of UV-C light (0–10.6 kJ/m2) assisted by mild heat treatment (50 °C) on the inactivation of Saccharomyces cerevisiae KE 162 in peptone water and fresh carrot-orange juice blend (pH: 3.8; 9.8°Brix; 707 NTU; absorption coefficient: 0.17 cm−1). Yeast induced damage by single UV-C and mild heat (H) and the combined treatment UV-C/H, was investigated by flow cytometry (FC) and transmission electron microscopy (TEM). When studying induced damage by FC, cells were labeled with fluorescein diacetate (FDA) and propidium iodide (PI) to monitor membrane integrity and esterase activity. UV-C/H provoked up to 4.7 log-reductions of S. cerevisiae; whereas, only 2.6–3.3 log-reductions were achieved by single UV-C and H treatments. FC revealed a shift with treatment time from cells with esterase activity and intact membrane to cells with permeabilized membrane. This shift was more noticeable in peptone water and UV-C/H treated juice. In the UV-C treated juice, double stained cells were detected, suggesting the possibility of being sub-lethally damaged, with compromised membrane but still metabolically active. TEM images of treated cells revealed severe damage, encompassing coagulated inner content, disorganized lumen and cell debris. FC and TEM provided additional information regarding degree and type of damage, complementing information revealed by the traditional plate count technique. © 2018 Elsevier Ltd JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07400020_v73_n_p1_GarciaCarrillo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Flow cytometry Mild heat Transmission electron microscopy UV-C light carrot chemistry evaluation study flow cytometry food preservation fruit and vegetable juice growth, development and aging heat microbial viability microbiology procedures radiation response Saccharomyces cerevisiae sweet orange transmission electron microscopy ultrastructure ultraviolet radiation Citrus sinensis Daucus carota Flow Cytometry Food Preservation Fruit and Vegetable Juices Hot Temperature Microbial Viability Microscopy, Electron, Transmission Saccharomyces cerevisiae Ultraviolet Rays
spellingShingle	Flow cytometry Mild heat Transmission electron microscopy UV-C light carrot chemistry evaluation study flow cytometry food preservation fruit and vegetable juice growth, development and aging heat microbial viability microbiology procedures radiation response Saccharomyces cerevisiae sweet orange transmission electron microscopy ultrastructure ultraviolet radiation Citrus sinensis Daucus carota Flow Cytometry Food Preservation Fruit and Vegetable Juices Hot Temperature Microbial Viability Microscopy, Electron, Transmission Saccharomyces cerevisiae Ultraviolet Rays García Carrillo, M. Ferrario, M. Guerrero, S. Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy
topic_facet	Flow cytometry Mild heat Transmission electron microscopy UV-C light carrot chemistry evaluation study flow cytometry food preservation fruit and vegetable juice growth, development and aging heat microbial viability microbiology procedures radiation response Saccharomyces cerevisiae sweet orange transmission electron microscopy ultrastructure ultraviolet radiation Citrus sinensis Daucus carota Flow Cytometry Food Preservation Fruit and Vegetable Juices Hot Temperature Microbial Viability Microscopy, Electron, Transmission Saccharomyces cerevisiae Ultraviolet Rays
description	The aim of this study was to analyze the effectiveness of UV-C light (0–10.6 kJ/m2) assisted by mild heat treatment (50 °C) on the inactivation of Saccharomyces cerevisiae KE 162 in peptone water and fresh carrot-orange juice blend (pH: 3.8; 9.8°Brix; 707 NTU; absorption coefficient: 0.17 cm−1). Yeast induced damage by single UV-C and mild heat (H) and the combined treatment UV-C/H, was investigated by flow cytometry (FC) and transmission electron microscopy (TEM). When studying induced damage by FC, cells were labeled with fluorescein diacetate (FDA) and propidium iodide (PI) to monitor membrane integrity and esterase activity. UV-C/H provoked up to 4.7 log-reductions of S. cerevisiae; whereas, only 2.6–3.3 log-reductions were achieved by single UV-C and H treatments. FC revealed a shift with treatment time from cells with esterase activity and intact membrane to cells with permeabilized membrane. This shift was more noticeable in peptone water and UV-C/H treated juice. In the UV-C treated juice, double stained cells were detected, suggesting the possibility of being sub-lethally damaged, with compromised membrane but still metabolically active. TEM images of treated cells revealed severe damage, encompassing coagulated inner content, disorganized lumen and cell debris. FC and TEM provided additional information regarding degree and type of damage, complementing information revealed by the traditional plate count technique. © 2018 Elsevier Ltd
format	JOUR
author	García Carrillo, M. Ferrario, M. Guerrero, S.
author_facet	García Carrillo, M. Ferrario, M. Guerrero, S.
author_sort	García Carrillo, M.
title	Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy
title_short	Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy
title_full	Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy
title_fullStr	Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy
title_full_unstemmed	Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy
title_sort	effectiveness of uv-c light assisted by mild heat on saccharomyces cerevisiae ke 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy
url	http://hdl.handle.net/20.500.12110/paper_07400020_v73_n_p1_GarciaCarrillo
work_keys_str_mv	AT garciacarrillom effectivenessofuvclightassistedbymildheatonsaccharomycescerevisiaeke162inactivationincarrotorangejuiceblendstudiedbyflowcytometryandtransmissionelectronmicroscopy AT ferrariom effectivenessofuvclightassistedbymildheatonsaccharomycescerevisiaeke162inactivationincarrotorangejuiceblendstudiedbyflowcytometryandtransmissionelectronmicroscopy AT guerreros effectivenessofuvclightassistedbymildheatonsaccharomycescerevisiaeke162inactivationincarrotorangejuiceblendstudiedbyflowcytometryandtransmissionelectronmicroscopy
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Effectiveness of UV-C light assisted by mild heat on Saccharomyces cerevisiae KE 162 inactivation in carrot-orange juice blend studied by flow cytometry and transmission electron microscopy

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