Recurrent amplifications and deletions of satellite DNA accompanied chromosomal diversification in South American tuco-tucos (genus Ctenomys, Rodentia: Octodontidae): A phylogenetic approach
We investigated the relationship between satellite copy number and chromosomal evolution in tuco-tucos (genus Ctenomys), a karyotypically diverse clade of rodents. To explore phylogenetic relationships among 23 species and 5 undescribed forms, we sequenced the complete mitochondrial cytochrome b gen...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_07374038_v18_n9_p1708_Slamovits |
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todo:paper_07374038_v18_n9_p1708_Slamovits2023-10-03T15:37:56Z Recurrent amplifications and deletions of satellite DNA accompanied chromosomal diversification in South American tuco-tucos (genus Ctenomys, Rodentia: Octodontidae): A phylogenetic approach Slamovits, C.H. Cook, J.A. Lessa, E.P. Rossi, M.S. Chromosomal evolution Cytochrome b Phylogenetics Satellite DNA satellite DNA article chromosome chromosome rearrangement dot hybridization gene amplification gene deletion gene sequence genetic stability karyotype molecular evolution nonhuman nucleotide sequence phylogeny rodent sequence analysis species differentiation unindexed sequence Ctenomys We investigated the relationship between satellite copy number and chromosomal evolution in tuco-tucos (genus Ctenomys), a karyotypically diverse clade of rodents. To explore phylogenetic relationships among 23 species and 5 undescribed forms, we sequenced the complete mitochondrial cytochrome b genes of 27 specimens and incorporated 27 previously published sequences. We then used quantitative dot-blot techniques to assess changes in the copy number of the major Ctenomys satellite DNA (satDNA), named RPCS. Our analysis of the relationship between variation in copy number of RPCS and chromosomal changes employed a maximum-likelihood approach to infer the copy number of the satellite RPCS in the ancestors of each clade. We found that amplifications and deletions of RPCS were associated with extensive chromosomal rearrangements even among closely related species. In contrast, RPCS copy number stability was observed within clades characterized by chromosomal stability. This example reinforces the suspected role of amplification, deletion, and intragenomic movement of satDNA in promoting extensive chromosomal evolution. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07374038_v18_n9_p1708_Slamovits |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chromosomal evolution Cytochrome b Phylogenetics Satellite DNA satellite DNA article chromosome chromosome rearrangement dot hybridization gene amplification gene deletion gene sequence genetic stability karyotype molecular evolution nonhuman nucleotide sequence phylogeny rodent sequence analysis species differentiation unindexed sequence Ctenomys |
| spellingShingle |
Chromosomal evolution Cytochrome b Phylogenetics Satellite DNA satellite DNA article chromosome chromosome rearrangement dot hybridization gene amplification gene deletion gene sequence genetic stability karyotype molecular evolution nonhuman nucleotide sequence phylogeny rodent sequence analysis species differentiation unindexed sequence Ctenomys Slamovits, C.H. Cook, J.A. Lessa, E.P. Rossi, M.S. Recurrent amplifications and deletions of satellite DNA accompanied chromosomal diversification in South American tuco-tucos (genus Ctenomys, Rodentia: Octodontidae): A phylogenetic approach |
| topic_facet |
Chromosomal evolution Cytochrome b Phylogenetics Satellite DNA satellite DNA article chromosome chromosome rearrangement dot hybridization gene amplification gene deletion gene sequence genetic stability karyotype molecular evolution nonhuman nucleotide sequence phylogeny rodent sequence analysis species differentiation unindexed sequence Ctenomys |
| description |
We investigated the relationship between satellite copy number and chromosomal evolution in tuco-tucos (genus Ctenomys), a karyotypically diverse clade of rodents. To explore phylogenetic relationships among 23 species and 5 undescribed forms, we sequenced the complete mitochondrial cytochrome b genes of 27 specimens and incorporated 27 previously published sequences. We then used quantitative dot-blot techniques to assess changes in the copy number of the major Ctenomys satellite DNA (satDNA), named RPCS. Our analysis of the relationship between variation in copy number of RPCS and chromosomal changes employed a maximum-likelihood approach to infer the copy number of the satellite RPCS in the ancestors of each clade. We found that amplifications and deletions of RPCS were associated with extensive chromosomal rearrangements even among closely related species. In contrast, RPCS copy number stability was observed within clades characterized by chromosomal stability. This example reinforces the suspected role of amplification, deletion, and intragenomic movement of satDNA in promoting extensive chromosomal evolution. |
| format |
JOUR |
| author |
Slamovits, C.H. Cook, J.A. Lessa, E.P. Rossi, M.S. |
| author_facet |
Slamovits, C.H. Cook, J.A. Lessa, E.P. Rossi, M.S. |
| author_sort |
Slamovits, C.H. |
| title |
Recurrent amplifications and deletions of satellite DNA accompanied chromosomal diversification in South American tuco-tucos (genus Ctenomys, Rodentia: Octodontidae): A phylogenetic approach |
| title_short |
Recurrent amplifications and deletions of satellite DNA accompanied chromosomal diversification in South American tuco-tucos (genus Ctenomys, Rodentia: Octodontidae): A phylogenetic approach |
| title_full |
Recurrent amplifications and deletions of satellite DNA accompanied chromosomal diversification in South American tuco-tucos (genus Ctenomys, Rodentia: Octodontidae): A phylogenetic approach |
| title_fullStr |
Recurrent amplifications and deletions of satellite DNA accompanied chromosomal diversification in South American tuco-tucos (genus Ctenomys, Rodentia: Octodontidae): A phylogenetic approach |
| title_full_unstemmed |
Recurrent amplifications and deletions of satellite DNA accompanied chromosomal diversification in South American tuco-tucos (genus Ctenomys, Rodentia: Octodontidae): A phylogenetic approach |
| title_sort |
recurrent amplifications and deletions of satellite dna accompanied chromosomal diversification in south american tuco-tucos (genus ctenomys, rodentia: octodontidae): a phylogenetic approach |
| url |
http://hdl.handle.net/20.500.12110/paper_07374038_v18_n9_p1708_Slamovits |
| work_keys_str_mv |
AT slamovitsch recurrentamplificationsanddeletionsofsatellitednaaccompaniedchromosomaldiversificationinsouthamericantucotucosgenusctenomysrodentiaoctodontidaeaphylogeneticapproach AT cookja recurrentamplificationsanddeletionsofsatellitednaaccompaniedchromosomaldiversificationinsouthamericantucotucosgenusctenomysrodentiaoctodontidaeaphylogeneticapproach AT lessaep recurrentamplificationsanddeletionsofsatellitednaaccompaniedchromosomaldiversificationinsouthamericantucotucosgenusctenomysrodentiaoctodontidaeaphylogeneticapproach AT rossims recurrentamplificationsanddeletionsofsatellitednaaccompaniedchromosomaldiversificationinsouthamericantucotucosgenusctenomysrodentiaoctodontidaeaphylogeneticapproach |
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1782029944649416704 |