αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms

Multiple transforming growth factors (TGFs) capable of conferring the neoplastic phenotype on NRK-49F cells without the addition of any other exogenous growth factor in the soft agar assay, were purified from two human solid malignant neoplasms: a squamous lung carcinoma and a pectoral rhabdomyosarc...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Elizalde, P.V., Charreau, E.H.
Formato: JOUR
Materias:
transforming growth factor
article
cancer cell
carcinoma
cell culture
cell growth
dna synthesis
human
human cell
priority journal
sarcoma
Carcinoma, Squamous Cell
Cell Transformation, Neoplastic
Cells, Cultured
Chromatography, Gel
DNA
Dose-Response Relationship, Drug
Fibroblasts
Human
In Vitro
Lung Neoplasms
Receptor, Epidermal Growth Factor
Rhabdomyosarcoma
Support, Non-U.S. Gov't
Thoracic Neoplasms
Transforming Growth Factor alpha
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_07357907_v8_n3-4_p365_Elizalde
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_07357907_v8_n3-4_p365_Elizalde
record_format dspace
spelling todo:paper_07357907_v8_n3-4_p365_Elizalde2023-10-03T15:37:46Z αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms Elizalde, P.V. Charreau, E.H. transforming growth factor article cancer cell carcinoma cell culture cell growth dna synthesis human human cell priority journal sarcoma Carcinoma, Squamous Cell Cell Transformation, Neoplastic Cells, Cultured Chromatography, Gel DNA Dose-Response Relationship, Drug Fibroblasts Human In Vitro Lung Neoplasms Receptor, Epidermal Growth Factor Rhabdomyosarcoma Support, Non-U.S. Gov't Thoracic Neoplasms Transforming Growth Factor alpha Multiple transforming growth factors (TGFs) capable of conferring the neoplastic phenotype on NRK-49F cells without the addition of any other exogenous growth factor in the soft agar assay, were purified from two human solid malignant neoplasms: a squamous lung carcinoma and a pectoral rhabdomyosarcoma. In both tumors, low-molecular-weight transforming activities (4000-6000) that were not potentiated by epidermal growth factor (EGF), competed for binding to the EGF receptor, possessed mitogenic activity on NRK fibroblasts arrested in serum-deprived medium, and did not show inhibitory effects on DNA synthesis induced by EGF and insulin in NRK cells. Other TGFs with molecular weights 9000 to 48,000, were also found in the malignant tissues examined; these TGFs, were not potentiated by EGF, did not compete for binding to the EGF receptor, were not mitogenic for NRK cells, and acted as potent inhibitors of DNA synthesis induced by EGF and insulin in NRK cells. These results demonstrate that growth-promoting activities, and modulating agents that can act as either enhancers or inhibitors of cell proliferation, are present in neoplastic tissues of different embryologic origin and histologic type. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07357907_v8_n3-4_p365_Elizalde
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic transforming growth factor
article
cancer cell
carcinoma
cell culture
cell growth
dna synthesis
human
human cell
priority journal
sarcoma
Carcinoma, Squamous Cell
Cell Transformation, Neoplastic
Cells, Cultured
Chromatography, Gel
DNA
Dose-Response Relationship, Drug
Fibroblasts
Human
In Vitro
Lung Neoplasms
Receptor, Epidermal Growth Factor
Rhabdomyosarcoma
Support, Non-U.S. Gov't
Thoracic Neoplasms
Transforming Growth Factor alpha
spellingShingle transforming growth factor
article
cancer cell
carcinoma
cell culture
cell growth
dna synthesis
human
human cell
priority journal
sarcoma
Carcinoma, Squamous Cell
Cell Transformation, Neoplastic
Cells, Cultured
Chromatography, Gel
DNA
Dose-Response Relationship, Drug
Fibroblasts
Human
In Vitro
Lung Neoplasms
Receptor, Epidermal Growth Factor
Rhabdomyosarcoma
Support, Non-U.S. Gov't
Thoracic Neoplasms
Transforming Growth Factor alpha
Elizalde, P.V.
Charreau, E.H.
αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms
topic_facet transforming growth factor
article
cancer cell
carcinoma
cell culture
cell growth
dna synthesis
human
human cell
priority journal
sarcoma
Carcinoma, Squamous Cell
Cell Transformation, Neoplastic
Cells, Cultured
Chromatography, Gel
DNA
Dose-Response Relationship, Drug
Fibroblasts
Human
In Vitro
Lung Neoplasms
Receptor, Epidermal Growth Factor
Rhabdomyosarcoma
Support, Non-U.S. Gov't
Thoracic Neoplasms
Transforming Growth Factor alpha
description Multiple transforming growth factors (TGFs) capable of conferring the neoplastic phenotype on NRK-49F cells without the addition of any other exogenous growth factor in the soft agar assay, were purified from two human solid malignant neoplasms: a squamous lung carcinoma and a pectoral rhabdomyosarcoma. In both tumors, low-molecular-weight transforming activities (4000-6000) that were not potentiated by epidermal growth factor (EGF), competed for binding to the EGF receptor, possessed mitogenic activity on NRK fibroblasts arrested in serum-deprived medium, and did not show inhibitory effects on DNA synthesis induced by EGF and insulin in NRK cells. Other TGFs with molecular weights 9000 to 48,000, were also found in the malignant tissues examined; these TGFs, were not potentiated by EGF, did not compete for binding to the EGF receptor, were not mitogenic for NRK cells, and acted as potent inhibitors of DNA synthesis induced by EGF and insulin in NRK cells. These results demonstrate that growth-promoting activities, and modulating agents that can act as either enhancers or inhibitors of cell proliferation, are present in neoplastic tissues of different embryologic origin and histologic type. © 1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
format JOUR
author Elizalde, P.V.
Charreau, E.H.
author_facet Elizalde, P.V.
Charreau, E.H.
author_sort Elizalde, P.V.
title αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms
title_short αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms
title_full αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms
title_fullStr αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms
title_full_unstemmed αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms
title_sort αtransforming growth factorlike activities and bifunctional regulators of cell growth in human malignant neoplasms
url http://hdl.handle.net/20.500.12110/paper_07357907_v8_n3-4_p365_Elizalde
work_keys_str_mv AT elizaldepv atransforminggrowthfactorlikeactivitiesandbifunctionalregulatorsofcellgrowthinhumanmalignantneoplasms
AT charreaueh atransforminggrowthfactorlikeactivitiesandbifunctionalregulatorsofcellgrowthinhumanmalignantneoplasms
_version_ 1807316283727806464

Ejemplares similares