Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana
Key message: The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection againstSclerotiniasclerotiorumandRhizoctonia solani. Abstract: Germin-like proteins (GLPs) are a large, diverse and ubiquitou...
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todo:paper_07217714_v34_n10_p1717_Beracochea2023-10-03T15:36:51Z Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana Beracochea, V.C. Almasia, N.I. Peluffo, L. Nahirñak, V. Hopp, E.H. Paniego, N. Heinz, R.A. Vazquez-Rovere, C. Lia, V.V. Arabidopsis thaliana Germin-like protein Rhizoctonia solani ROS accumulation Sclerotinia sclerotiorum Sunflower germin glycoprotein reactive oxygen metabolite vegetable protein Arabidopsis Ascomycetes gene expression regulation genetics metabolism microbiology physiology plant disease sunflower transgenic plant Arabidopsis Ascomycota Gene Expression Regulation, Plant Glycoproteins Helianthus Plant Diseases Plant Proteins Plants, Genetically Modified Reactive Oxygen Species Key message: The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection againstSclerotiniasclerotiorumandRhizoctonia solani. Abstract: Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoproteins belonging to the Cupin super family. These proteins have been widely studied because of their diverse roles in important plant processes, including defence. The novel sunflower gene HaGLP1 encodes the first germin-like protein characterized from the family Asteraceae. To analyse whether constitutive in vivo expression of the HaGLP1 gene may lead to disease tolerance, we developed transgenic Arabidopsis plants that were molecularly characterized and biologically assessed after inoculation with Sclerotinia sclerotiorum or Rhizoctonia solani.HaGLP1 expression in Arabidopsis plants conferred tolerance to S. sclerotiorum at the first stages of disease and interfered with R. solani infection, thus giving rise to significant protection against the latter. Furthermore, HaGLP1 expression in Arabidopsis plants elevated endogenous ROS levels. HaGLP1-induced tolerance does not appear to be related to a constitutive induction of the plant defence or the ROS-related genes examined here. In conclusion, our data suggest that HaGLP1 is an interesting candidate for the engineering of plants with increased fungal tolerance and that this gene could also be useful for the selection of naturally overexpressing sunflower genotypes for conventional breeding purposes. © 2015, Springer-Verlag Berlin Heidelberg. Fil:Almasia, N.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Peluffo, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vazquez-Rovere, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lia, V.V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07217714_v34_n10_p1717_Beracochea |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arabidopsis thaliana Germin-like protein Rhizoctonia solani ROS accumulation Sclerotinia sclerotiorum Sunflower germin glycoprotein reactive oxygen metabolite vegetable protein Arabidopsis Ascomycetes gene expression regulation genetics metabolism microbiology physiology plant disease sunflower transgenic plant Arabidopsis Ascomycota Gene Expression Regulation, Plant Glycoproteins Helianthus Plant Diseases Plant Proteins Plants, Genetically Modified Reactive Oxygen Species |
| spellingShingle |
Arabidopsis thaliana Germin-like protein Rhizoctonia solani ROS accumulation Sclerotinia sclerotiorum Sunflower germin glycoprotein reactive oxygen metabolite vegetable protein Arabidopsis Ascomycetes gene expression regulation genetics metabolism microbiology physiology plant disease sunflower transgenic plant Arabidopsis Ascomycota Gene Expression Regulation, Plant Glycoproteins Helianthus Plant Diseases Plant Proteins Plants, Genetically Modified Reactive Oxygen Species Beracochea, V.C. Almasia, N.I. Peluffo, L. Nahirñak, V. Hopp, E.H. Paniego, N. Heinz, R.A. Vazquez-Rovere, C. Lia, V.V. Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| topic_facet |
Arabidopsis thaliana Germin-like protein Rhizoctonia solani ROS accumulation Sclerotinia sclerotiorum Sunflower germin glycoprotein reactive oxygen metabolite vegetable protein Arabidopsis Ascomycetes gene expression regulation genetics metabolism microbiology physiology plant disease sunflower transgenic plant Arabidopsis Ascomycota Gene Expression Regulation, Plant Glycoproteins Helianthus Plant Diseases Plant Proteins Plants, Genetically Modified Reactive Oxygen Species |
| description |
Key message: The novel sunflower gene HaGLP1 is the first germin-like protein characterized from the family Asteraceae. It alters the host redox status and confers protection againstSclerotiniasclerotiorumandRhizoctonia solani. Abstract: Germin-like proteins (GLPs) are a large, diverse and ubiquitous family of plant glycoproteins belonging to the Cupin super family. These proteins have been widely studied because of their diverse roles in important plant processes, including defence. The novel sunflower gene HaGLP1 encodes the first germin-like protein characterized from the family Asteraceae. To analyse whether constitutive in vivo expression of the HaGLP1 gene may lead to disease tolerance, we developed transgenic Arabidopsis plants that were molecularly characterized and biologically assessed after inoculation with Sclerotinia sclerotiorum or Rhizoctonia solani.HaGLP1 expression in Arabidopsis plants conferred tolerance to S. sclerotiorum at the first stages of disease and interfered with R. solani infection, thus giving rise to significant protection against the latter. Furthermore, HaGLP1 expression in Arabidopsis plants elevated endogenous ROS levels. HaGLP1-induced tolerance does not appear to be related to a constitutive induction of the plant defence or the ROS-related genes examined here. In conclusion, our data suggest that HaGLP1 is an interesting candidate for the engineering of plants with increased fungal tolerance and that this gene could also be useful for the selection of naturally overexpressing sunflower genotypes for conventional breeding purposes. © 2015, Springer-Verlag Berlin Heidelberg. |
| format |
JOUR |
| author |
Beracochea, V.C. Almasia, N.I. Peluffo, L. Nahirñak, V. Hopp, E.H. Paniego, N. Heinz, R.A. Vazquez-Rovere, C. Lia, V.V. |
| author_facet |
Beracochea, V.C. Almasia, N.I. Peluffo, L. Nahirñak, V. Hopp, E.H. Paniego, N. Heinz, R.A. Vazquez-Rovere, C. Lia, V.V. |
| author_sort |
Beracochea, V.C. |
| title |
Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title_short |
Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title_full |
Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title_fullStr |
Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title_full_unstemmed |
Sunflower germin-like protein HaGLP1 promotes ROS accumulation and enhances protection against fungal pathogens in transgenic Arabidopsis thaliana |
| title_sort |
sunflower germin-like protein haglp1 promotes ros accumulation and enhances protection against fungal pathogens in transgenic arabidopsis thaliana |
| url |
http://hdl.handle.net/20.500.12110/paper_07217714_v34_n10_p1717_Beracochea |
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1807324245654503424 |