Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)

The promoter region of two sunflower (Helianthus annuus L. HA89 genotype) seed specifically expressed genes, coding for an oleate desaturase (HaFAD2-1) and a lipid transfer protein (HaAP10), were cloned and in silico characterized. The isolated fragments are 867 and 964 bp long, respectively, and co...

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Autores principales:	Zavallo, D., Bilbao, M.L., Hopp, H.E., Heinz, R.
Formato:	JOUR
Materias:	Lipid transfer protein Oleate desaturase Seed promoter Sunflower Arabidopsis Helianthus Helianthus annuus acyl coenzyme A desaturase carrier protein lipid transfer protein oxidoreductase Phosphatidylcholine desaturase plant RNA vegetable protein article gene expression profiling gene expression regulation genetics molecular cloning plant gene plant seed promoter region sunflower transgenic plant Carrier Proteins Cloning, Molecular Fatty Acid Desaturases Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Oxidoreductases Acting on CH-CH Group Donors Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Seeds
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_07217714_v29_n3_p239_Zavallo
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_07217714_v29_n3_p239_Zavallo
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spelling	todo:paper_07217714_v29_n3_p239_Zavallo2023-10-03T15:36:49Z Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.) Zavallo, D. Bilbao, M.L. Hopp, H.E. Heinz, R. Lipid transfer protein Oleate desaturase Seed promoter Sunflower Arabidopsis Helianthus Helianthus annuus acyl coenzyme A desaturase carrier protein lipid transfer protein oxidoreductase Phosphatidylcholine desaturase plant RNA vegetable protein Arabidopsis article gene expression profiling gene expression regulation genetics molecular cloning plant gene plant seed promoter region sunflower transgenic plant Arabidopsis Carrier Proteins Cloning, Molecular Fatty Acid Desaturases Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Helianthus Oxidoreductases Acting on CH-CH Group Donors Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Seeds The promoter region of two sunflower (Helianthus annuus L. HA89 genotype) seed specifically expressed genes, coding for an oleate desaturase (HaFAD2-1) and a lipid transfer protein (HaAP10), were cloned and in silico characterized. The isolated fragments are 867 and 964 bp long, respectively, and contain several seed-specific motifs, such as AACA motif, ACGT element, E-Boxes, SEF binding sites and GCN4 motif. Functional analysis of these promoters in transgenic Arabidopsis plants was investigated after fusing them with the β-glucuronidase (GUS) reporter gene. None of the promoters triggered GUS activity in any vegetative tissue, with the exception of early seedling cotyledons. HaFAD2-1 and HaAP10 promoters were tested along seed development from globular stage to mature seeds. GUS staining was restricted to embryonic tissue and quantitative fluorometric assays showed high activity values at the later stages of development. In this work we demonstrate that HaFAD2-1 promoter is as strong as 35S promoter even though it is a tissue-specific promoter and its activity derived just from the embryo, thus confirming that it can be considered a strong highly specific seed promoter useful for biotechnology applications. © 2010 Springer-Verlag. Fil:Zavallo, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Hopp, H.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Heinz, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_07217714_v29_n3_p239_Zavallo
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Lipid transfer protein Oleate desaturase Seed promoter Sunflower Arabidopsis Helianthus Helianthus annuus acyl coenzyme A desaturase carrier protein lipid transfer protein oxidoreductase Phosphatidylcholine desaturase plant RNA vegetable protein Arabidopsis article gene expression profiling gene expression regulation genetics molecular cloning plant gene plant seed promoter region sunflower transgenic plant Arabidopsis Carrier Proteins Cloning, Molecular Fatty Acid Desaturases Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Helianthus Oxidoreductases Acting on CH-CH Group Donors Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Seeds
spellingShingle	Lipid transfer protein Oleate desaturase Seed promoter Sunflower Arabidopsis Helianthus Helianthus annuus acyl coenzyme A desaturase carrier protein lipid transfer protein oxidoreductase Phosphatidylcholine desaturase plant RNA vegetable protein Arabidopsis article gene expression profiling gene expression regulation genetics molecular cloning plant gene plant seed promoter region sunflower transgenic plant Arabidopsis Carrier Proteins Cloning, Molecular Fatty Acid Desaturases Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Helianthus Oxidoreductases Acting on CH-CH Group Donors Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Seeds Zavallo, D. Bilbao, M.L. Hopp, H.E. Heinz, R. Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)
topic_facet	Lipid transfer protein Oleate desaturase Seed promoter Sunflower Arabidopsis Helianthus Helianthus annuus acyl coenzyme A desaturase carrier protein lipid transfer protein oxidoreductase Phosphatidylcholine desaturase plant RNA vegetable protein Arabidopsis article gene expression profiling gene expression regulation genetics molecular cloning plant gene plant seed promoter region sunflower transgenic plant Arabidopsis Carrier Proteins Cloning, Molecular Fatty Acid Desaturases Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Helianthus Oxidoreductases Acting on CH-CH Group Donors Plant Proteins Plants, Genetically Modified Promoter Regions, Genetic RNA, Plant Seeds
description	The promoter region of two sunflower (Helianthus annuus L. HA89 genotype) seed specifically expressed genes, coding for an oleate desaturase (HaFAD2-1) and a lipid transfer protein (HaAP10), were cloned and in silico characterized. The isolated fragments are 867 and 964 bp long, respectively, and contain several seed-specific motifs, such as AACA motif, ACGT element, E-Boxes, SEF binding sites and GCN4 motif. Functional analysis of these promoters in transgenic Arabidopsis plants was investigated after fusing them with the β-glucuronidase (GUS) reporter gene. None of the promoters triggered GUS activity in any vegetative tissue, with the exception of early seedling cotyledons. HaFAD2-1 and HaAP10 promoters were tested along seed development from globular stage to mature seeds. GUS staining was restricted to embryonic tissue and quantitative fluorometric assays showed high activity values at the later stages of development. In this work we demonstrate that HaFAD2-1 promoter is as strong as 35S promoter even though it is a tissue-specific promoter and its activity derived just from the embryo, thus confirming that it can be considered a strong highly specific seed promoter useful for biotechnology applications. © 2010 Springer-Verlag.
format	JOUR
author	Zavallo, D. Bilbao, M.L. Hopp, H.E. Heinz, R.
author_facet	Zavallo, D. Bilbao, M.L. Hopp, H.E. Heinz, R.
author_sort	Zavallo, D.
title	Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)
title_short	Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)
title_full	Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)
title_fullStr	Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)
title_full_unstemmed	Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)
title_sort	isolation and functional characterization of two novel seed-specific promoters from sunflower (helianthus annuus l.)
url	http://hdl.handle.net/20.500.12110/paper_07217714_v29_n3_p239_Zavallo
work_keys_str_mv	AT zavallod isolationandfunctionalcharacterizationoftwonovelseedspecificpromotersfromsunflowerhelianthusannuusl AT bilbaoml isolationandfunctionalcharacterizationoftwonovelseedspecificpromotersfromsunflowerhelianthusannuusl AT hopphe isolationandfunctionalcharacterizationoftwonovelseedspecificpromotersfromsunflowerhelianthusannuusl AT heinzr isolationandfunctionalcharacterizationoftwonovelseedspecificpromotersfromsunflowerhelianthusannuusl
_version_	1782027911086211072

Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)

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