Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS
Soluble sugars play a central role in metabolism and water relations, their analysis can provide important information about biochemistry of growth, flowering and exposure of plants to different stresses. Here we report a successful application of UV-MALDI-MS for direct profiling of soluble sugars o...
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| Formato: | SER |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_05677572_v923_n_p53_Nonami |
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todo:paper_05677572_v923_n_p53_Nonami2023-10-03T15:35:56Z Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS Nonami, H. Gholipour, Y. Erra-Balsells R. Mass spectrometry Soluble carbohydrate profiling Sugar Tulipa Soluble sugars play a central role in metabolism and water relations, their analysis can provide important information about biochemistry of growth, flowering and exposure of plants to different stresses. Here we report a successful application of UV-MALDI-MS for direct profiling of soluble sugars on the surface of tulip bulb tissue and also their detection in microsamples sucked from single cells of tulip bulbs by using Pressure Probe. Soluble neutral carbohydrates could be directly desorbed/ ionized by deposition of UV-MALDI matrix on the dried tissue. Despite the tiny volume of the single cell microsample sucked (picoliter) the high sensitivity of UVMALDI- MS allowed the detection of soluble sugars after its deposition on air-dried layers of different matrixes. Results obtained from intact tissue and single cell level experiments were comparable; similar sugars signals could be detected and also their relative signal intensity were similar in both experiments. SER info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_05677572_v923_n_p53_Nonami |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Mass spectrometry Soluble carbohydrate profiling Sugar Tulipa |
| spellingShingle |
Mass spectrometry Soluble carbohydrate profiling Sugar Tulipa Nonami, H. Gholipour, Y. Erra-Balsells R. Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS |
| topic_facet |
Mass spectrometry Soluble carbohydrate profiling Sugar Tulipa |
| description |
Soluble sugars play a central role in metabolism and water relations, their analysis can provide important information about biochemistry of growth, flowering and exposure of plants to different stresses. Here we report a successful application of UV-MALDI-MS for direct profiling of soluble sugars on the surface of tulip bulb tissue and also their detection in microsamples sucked from single cells of tulip bulbs by using Pressure Probe. Soluble neutral carbohydrates could be directly desorbed/ ionized by deposition of UV-MALDI matrix on the dried tissue. Despite the tiny volume of the single cell microsample sucked (picoliter) the high sensitivity of UVMALDI- MS allowed the detection of soluble sugars after its deposition on air-dried layers of different matrixes. Results obtained from intact tissue and single cell level experiments were comparable; similar sugars signals could be detected and also their relative signal intensity were similar in both experiments. |
| format |
SER |
| author |
Nonami, H. Gholipour, Y. Erra-Balsells R. |
| author_facet |
Nonami, H. Gholipour, Y. Erra-Balsells R. |
| author_sort |
Nonami, H. |
| title |
Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS |
| title_short |
Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS |
| title_full |
Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS |
| title_fullStr |
Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS |
| title_full_unstemmed |
Direct tissue and cell analysis of tulip bulbs by combining pressure probe and UV-MALDI-MS |
| title_sort |
direct tissue and cell analysis of tulip bulbs by combining pressure probe and uv-maldi-ms |
| url |
http://hdl.handle.net/20.500.12110/paper_05677572_v923_n_p53_Nonami |
| work_keys_str_mv |
AT nonamih directtissueandcellanalysisoftulipbulbsbycombiningpressureprobeanduvmaldims AT gholipoury directtissueandcellanalysisoftulipbulbsbycombiningpressureprobeanduvmaldims AT errabalsellsr directtissueandcellanalysisoftulipbulbsbycombiningpressureprobeanduvmaldims |
| _version_ |
1807320195721592832 |