Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue

The effect of the in vivo administration of hexachlorobenzene (HCB) (100 mg/100 g bw) for 30 days, on the activities of brown adipose tissue (BAT) lipogenic enzymes, i.e. malic enzyme (ME), and glucose-6-phosphate dehydrogenase (G6PD) and the mitochondrial non lipogenic enzyme, L-glycerol-3-phosphat...

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Autores principales:	Alvarez, L., Randi, A., Alvarez, P., Kölliker Frers, R., Kleiman De Pisarev, D.L.
Formato:	JOUR
Materias:	Brown adipose tissue Glucose-6-phosphate dehydrogenase Hexachlorobenzene L-glycerol-3-phosphate dehydrogenase Lipogenic enzymes Liver Malic enzyme, Thyroid hormones glucose 6 phosphate dehydrogenase glycerol 3 phosphate dehydrogenase hexachlorobenzene liothyronine malate dehydrogenase (decarboxylating) reduced nicotinamide adenine dinucleotide phosphate thyroid hormone animal cell animal experiment animal model animal tissue article brown adipose tissue controlled study energy balance enzyme activity hypothyroidism lipogenesis male nonhuman rat thyroidectomy time
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03914097_v22_n6_p436_Alvarez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03914097_v22_n6_p436_Alvarez
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spelling	todo:paper_03914097_v22_n6_p436_Alvarez2023-10-03T15:33:49Z Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue Alvarez, L. Randi, A. Alvarez, P. Kölliker Frers, R. Kleiman De Pisarev, D.L. Brown adipose tissue Glucose-6-phosphate dehydrogenase Hexachlorobenzene L-glycerol-3-phosphate dehydrogenase Lipogenic enzymes Liver Malic enzyme, Thyroid hormones glucose 6 phosphate dehydrogenase glycerol 3 phosphate dehydrogenase hexachlorobenzene liothyronine malate dehydrogenase (decarboxylating) reduced nicotinamide adenine dinucleotide phosphate thyroid hormone animal cell animal experiment animal model animal tissue article brown adipose tissue controlled study energy balance enzyme activity hypothyroidism lipogenesis male nonhuman rat thyroidectomy time The effect of the in vivo administration of hexachlorobenzene (HCB) (100 mg/100 g bw) for 30 days, on the activities of brown adipose tissue (BAT) lipogenic enzymes, i.e. malic enzyme (ME), and glucose-6-phosphate dehydrogenase (G6PD) and the mitochondrial non lipogenic enzyme, L-glycerol-3-phosphate dehydrogenase (LG3PD), was studied in male Wistar rats, submitted to various neurohormonal manipulations. BAT ME, G6PD and LG3PD activities showed significant reductions in response to HCB treatment both in euthyroid and surgically thyroidectomized rats, showing that the effect does not depend on the presence of thyroid hormones. These results differ from those obtained for hepatic ME and G6PD activities, which increased in HCB intoxicated rats without alteration in LG3PD. HCB decreased BAT ME activity under BAT denervation. Administration of HCB resulted in time and dose-dependent decreases in the activity of BAT malic enzyme. The basal activity of ME was increased in hypothyroid rats, while that of LG3PD was reduced. A stimulatory effect of receptor-saturating doses of triiodothyronine (T 3 ) (50 μg/100 g body weight) was observed on BAT ME and LG3PD activities in thyroidectomized rats, showing that the enzymes responded to thyroid hormone stimulation in a normal manner. The stimulatory effect of saturating doses of T 3 on ME and LG3PD was reduced by HCB. The results presented herein unequivocally show that brown adipose tissue is a specific target in HCB-induced toxicity, which in turn involves severe alterations in the regulation of BAT lipogenesis. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03914097_v22_n6_p436_Alvarez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Brown adipose tissue Glucose-6-phosphate dehydrogenase Hexachlorobenzene L-glycerol-3-phosphate dehydrogenase Lipogenic enzymes Liver Malic enzyme, Thyroid hormones glucose 6 phosphate dehydrogenase glycerol 3 phosphate dehydrogenase hexachlorobenzene liothyronine malate dehydrogenase (decarboxylating) reduced nicotinamide adenine dinucleotide phosphate thyroid hormone animal cell animal experiment animal model animal tissue article brown adipose tissue controlled study energy balance enzyme activity hypothyroidism lipogenesis male nonhuman rat thyroidectomy time
spellingShingle	Brown adipose tissue Glucose-6-phosphate dehydrogenase Hexachlorobenzene L-glycerol-3-phosphate dehydrogenase Lipogenic enzymes Liver Malic enzyme, Thyroid hormones glucose 6 phosphate dehydrogenase glycerol 3 phosphate dehydrogenase hexachlorobenzene liothyronine malate dehydrogenase (decarboxylating) reduced nicotinamide adenine dinucleotide phosphate thyroid hormone animal cell animal experiment animal model animal tissue article brown adipose tissue controlled study energy balance enzyme activity hypothyroidism lipogenesis male nonhuman rat thyroidectomy time Alvarez, L. Randi, A. Alvarez, P. Kölliker Frers, R. Kleiman De Pisarev, D.L. Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue
topic_facet	Brown adipose tissue Glucose-6-phosphate dehydrogenase Hexachlorobenzene L-glycerol-3-phosphate dehydrogenase Lipogenic enzymes Liver Malic enzyme, Thyroid hormones glucose 6 phosphate dehydrogenase glycerol 3 phosphate dehydrogenase hexachlorobenzene liothyronine malate dehydrogenase (decarboxylating) reduced nicotinamide adenine dinucleotide phosphate thyroid hormone animal cell animal experiment animal model animal tissue article brown adipose tissue controlled study energy balance enzyme activity hypothyroidism lipogenesis male nonhuman rat thyroidectomy time
description	The effect of the in vivo administration of hexachlorobenzene (HCB) (100 mg/100 g bw) for 30 days, on the activities of brown adipose tissue (BAT) lipogenic enzymes, i.e. malic enzyme (ME), and glucose-6-phosphate dehydrogenase (G6PD) and the mitochondrial non lipogenic enzyme, L-glycerol-3-phosphate dehydrogenase (LG3PD), was studied in male Wistar rats, submitted to various neurohormonal manipulations. BAT ME, G6PD and LG3PD activities showed significant reductions in response to HCB treatment both in euthyroid and surgically thyroidectomized rats, showing that the effect does not depend on the presence of thyroid hormones. These results differ from those obtained for hepatic ME and G6PD activities, which increased in HCB intoxicated rats without alteration in LG3PD. HCB decreased BAT ME activity under BAT denervation. Administration of HCB resulted in time and dose-dependent decreases in the activity of BAT malic enzyme. The basal activity of ME was increased in hypothyroid rats, while that of LG3PD was reduced. A stimulatory effect of receptor-saturating doses of triiodothyronine (T 3 ) (50 μg/100 g body weight) was observed on BAT ME and LG3PD activities in thyroidectomized rats, showing that the enzymes responded to thyroid hormone stimulation in a normal manner. The stimulatory effect of saturating doses of T 3 on ME and LG3PD was reduced by HCB. The results presented herein unequivocally show that brown adipose tissue is a specific target in HCB-induced toxicity, which in turn involves severe alterations in the regulation of BAT lipogenesis.
format	JOUR
author	Alvarez, L. Randi, A. Alvarez, P. Kölliker Frers, R. Kleiman De Pisarev, D.L.
author_facet	Alvarez, L. Randi, A. Alvarez, P. Kölliker Frers, R. Kleiman De Pisarev, D.L.
author_sort	Alvarez, L.
title	Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue
title_short	Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue
title_full	Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue
title_fullStr	Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue
title_full_unstemmed	Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue
title_sort	effect of hexachlorobenzene on nadph-generating lipogenic enzymes and l-glycerol-3-phosphate dehydrogenase in brown adipose tissue
url	http://hdl.handle.net/20.500.12110/paper_03914097_v22_n6_p436_Alvarez
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Effect of hexachlorobenzene on NADPH-generating lipogenic enzymes and L-glycerol-3-phosphate dehydrogenase in brown adipose tissue

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