Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad

A novel biospecific affinity chromatographic procedure was developed for the purification of the sex steroid binding protein from Bufo arenarum. A charcoal column connected in series to the affinity column allows the removal of any ligand non-covalently bound to the matrix or released during its sto...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Santa-Coloma, T.A., Garraffo, H.M., Gros, E.G., Charreau, E.H.
Formato:	JOUR
Materias:	sex hormone binding protein affinity chromatography animal cell methodology nonhuman priority journal protein purification toad Ammonium Sulfate Androstane-3,17-diol Animal Bufo arenarum Carrier Proteins Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Indicators and Reagents Protein Binding Sex Hormone-Binding Globulin Stanolone
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03784347_v415_nC_p297_SantaColoma
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

Ejemplares similares

Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
por: Santa Coloma, Tomás Antonio, et al.
Publicado: (1987)

Solid-phase assay for determination of binding parameters of ligand-protein complexes with high dissociation rates
por: Santa-Coloma, T.A., et al.

Solid-phase assay for determination of binding parameters of ligand-protein complexes with high dissociation rates
Publicado: (1991)

Sex steroid binding protein from Bufo arenarum: Further characterization
por: Santa-Coloma, T.A., et al.

Sex steroid binding protein from Bufo arenarum: Further characterization
Publicado: (1986)

Characterization of a sexual steroid binding protein in Bufo arenarum
por: Santa-Coloma, T.A., et al.

Characterization of a sexual steroid binding protein in Bufo arenarum
Publicado: (1985)

Studies on the reaction mechanism of soybean callus succinyl CoA synthetase. phosphorylation of the enzyme on immobilized adenosine-triphosphate and enzyme attached to a solid support
por: Batlle, Alcira María del Carmen
Publicado: (1972)

Studies on the reaction mechanism of soybean callus succinyl CoA synthetase. phosphorylation of the enzyme on immobilized adenosine-triphosphate and enzyme attached to a solid support
por: de Xifra, E.A.W., et al.
Publicado: (1972)

Studies on the reaction mechanism of soybean callus succinyl CoA synthetase. phosphorylation of the enzyme on immobilized adenosine-triphosphate and enzyme attached to a solid support
por: de Xifra, E.A.W., et al.

Studies on the reaction mechanism of soybean callus succinyl CoA synthetase. phosphorylation of the enzyme on immobilized adenosine-triphosphate and enzyme attached to a solid support
por: de Xifra, E.A.W., et al.
Publicado: (1972)

The allosteric modulation of thyroxine-binding globulin affinity is entropy driven
Publicado: (2013)

The allosteric modulation of thyroxine-binding globulin affinity is entropy driven
por: Petruk, A.A., et al.

Cyclic AMP-dependent protein kinase activity in Trypanosoma cruzi
Publicado: (1988)

Cyclic AMP-dependent protein kinase activity in Trypanosoma cruzi
por: Ulloa, R.M., et al.

Characteristics of aldosterone binding in rat and human serum
Publicado: (1982)

Characteristics of aldosterone binding in rat and human serum
por: Coirini, H., et al.

Antithrombin and first complement protein recognize the same active heparin fraction
por: Recondo, Eduardo Francisco
Publicado: (2002)

Antithrombin and first complement protein recognize the same active heparin fraction
por: Calabrese, G.C., et al.

Effects of mGnRH on testicular steroidogenesis in the toad Bufo arenarum
Publicado: (2002)

Effects of mGnRH on testicular steroidogenesis in the toad Bufo arenarum
por: Canosa, L.F., et al.

Association of ribosomal subunits: Mechanism of the reaction induced by association factor
por: González, Nélida Susana, et al.
Publicado: (1972)

Association of ribosomal subunits: Mechanism of the reaction induced by association factor
por: García-Patrone, M., et al.
Publicado: (1972)

Association of ribosomal subunits: Mechanism of the reaction induced by association factor
por: García-Patrone, M., et al.

Association of ribosomal subunits: Mechanism of the reaction induced by association factor
por: García-Patrone, M., et al.
Publicado: (1972)

SAnDReS 2.0: Development of machine-learning models to explore the scoring function space
por: de Azevedo, Walter Filgueira Jr., et al.
Publicado: (2024)

Cyclase-associated protein 1 (CAP1) is a prenyl-binding partner of Rap1 GTPase
Publicado: (2018)

Cyclase-associated protein 1 (CAP1) is a prenyl-binding partner of Rap1 GTPase
por: Zhang, X., et al.

Short linear motif core and flanking regions modulate retinoblastoma protein binding affinity and specificity
Publicado: (2018)

Short linear motif core and flanking regions modulate retinoblastoma protein binding affinity and specificity
por: Palopoli, N., et al.

Further evidence for the critical role of a non-chair conformation of L-iduronic acid in the activation of antithrombin
Publicado: (2002)

Further evidence for the critical role of a non-chair conformation of L-iduronic acid in the activation of antithrombin
por: Kovensky, J., et al.

Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol
por: Florin-Christensen, Jorge, et al.
Publicado: (1998)

Micellar lipoproteins as the possible storage and translocation form of intracellular diacylglycerol
por: Florin-Christensen, J., et al.

Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
por: Martínez, María Julia, et al.
Publicado: (2017)

Affinity chromatography matrices for depletion and purification of casein glycomacropeptide from bovine whey
por: Baieli, M.F., et al.

Retroviral-like features in the monomer of the major satellite DNA from the south american rodents of the genus ctenomys
por: Rossi, Maria Susana, et al.
Publicado: (1993)

Retroviral-like features in the monomer of the major satellite DNA from the south american rodents of the genus ctenomys
por: Rossi, M.S., et al.

Differential recruitment of tetratricorpeptide repeat domain immunophilins to the mineralocorticoid receptor influences both heat-shock protein 90-dependent retrotransport and hormone-dependent transcriptional activity
por: Gallo, Luciana Inés, et al.
Publicado: (2007)

Differential recruitment of tetratricorpeptide repeat domain immunophilins to the mineralocorticoid receptor influences both heat-shock protein 90-dependent retrotransport and hormone-dependent transcriptional activity
por: Gallo, L.I., et al.