Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad

A novel biospecific affinity chromatographic procedure was developed for the purification of the sex steroid binding protein from Bufo arenarum. A charcoal column connected in series to the affinity column allows the removal of any ligand non-covalently bound to the matrix or released during its sto...

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Autores principales: Santa-Coloma, T.A., Garraffo, H.M., Gros, E.G., Charreau, E.H.
Formato: JOUR
Materias:
sex hormone binding protein
affinity chromatography
animal cell
methodology
nonhuman
priority journal
protein purification
toad
Ammonium Sulfate
Androstane-3,17-diol
Animal
Bufo arenarum
Carrier Proteins
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Indicators and Reagents
Protein Binding
Sex Hormone-Binding Globulin
Stanolone
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03784347_v415_nC_p297_SantaColoma
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03784347_v415_nC_p297_SantaColoma
record_format dspace
spelling todo:paper_03784347_v415_nC_p297_SantaColoma2023-10-03T15:32:21Z Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad Santa-Coloma, T.A. Garraffo, H.M. Gros, E.G. Charreau, E.H. sex hormone binding protein affinity chromatography animal cell methodology nonhuman priority journal protein purification toad Ammonium Sulfate Androstane-3,17-diol Animal Bufo arenarum Carrier Proteins Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Indicators and Reagents Protein Binding Sex Hormone-Binding Globulin Stanolone A novel biospecific affinity chromatographic procedure was developed for the purification of the sex steroid binding protein from Bufo arenarum. A charcoal column connected in series to the affinity column allows the removal of any ligand non-covalently bound to the matrix or released during its storage, thus avoiding the need for exhaustive and prolonged washing procedures. In addition, it is not necessary to remove the endogenous ligand from the starting material and the binding to the affinity column can be monitored to determine the time required to achieve the maximum yield. The advantages are the charcoal adsorption of the ligand "washed" from the affinity column by the protein to be purified and the amplification provided by the cyclic use of the system. The procedure improves the yield from less than 1% (by conventional procedures) to more than 50%. With minor modifications this procedure can be useful for the purification of binding proteins and receptors. © 1987. Fil:Santa-Coloma, T.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Garraffo, H.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gros, E.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Charreau, E.H. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03784347_v415_nC_p297_SantaColoma
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic sex hormone binding protein
affinity chromatography
animal cell
methodology
nonhuman
priority journal
protein purification
toad
Ammonium Sulfate
Androstane-3,17-diol
Animal
Bufo arenarum
Carrier Proteins
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Indicators and Reagents
Protein Binding
Sex Hormone-Binding Globulin
Stanolone
spellingShingle sex hormone binding protein
affinity chromatography
animal cell
methodology
nonhuman
priority journal
protein purification
toad
Ammonium Sulfate
Androstane-3,17-diol
Animal
Bufo arenarum
Carrier Proteins
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Indicators and Reagents
Protein Binding
Sex Hormone-Binding Globulin
Stanolone
Santa-Coloma, T.A.
Garraffo, H.M.
Gros, E.G.
Charreau, E.H.
Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
topic_facet sex hormone binding protein
affinity chromatography
animal cell
methodology
nonhuman
priority journal
protein purification
toad
Ammonium Sulfate
Androstane-3,17-diol
Animal
Bufo arenarum
Carrier Proteins
Chromatography, Affinity
Electrophoresis, Polyacrylamide Gel
Indicators and Reagents
Protein Binding
Sex Hormone-Binding Globulin
Stanolone
description A novel biospecific affinity chromatographic procedure was developed for the purification of the sex steroid binding protein from Bufo arenarum. A charcoal column connected in series to the affinity column allows the removal of any ligand non-covalently bound to the matrix or released during its storage, thus avoiding the need for exhaustive and prolonged washing procedures. In addition, it is not necessary to remove the endogenous ligand from the starting material and the binding to the affinity column can be monitored to determine the time required to achieve the maximum yield. The advantages are the charcoal adsorption of the ligand "washed" from the affinity column by the protein to be purified and the amplification provided by the cyclic use of the system. The procedure improves the yield from less than 1% (by conventional procedures) to more than 50%. With minor modifications this procedure can be useful for the purification of binding proteins and receptors. © 1987.
format JOUR
author Santa-Coloma, T.A.
Garraffo, H.M.
Gros, E.G.
Charreau, E.H.
author_facet Santa-Coloma, T.A.
Garraffo, H.M.
Gros, E.G.
Charreau, E.H.
author_sort Santa-Coloma, T.A.
title Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
title_short Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
title_full Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
title_fullStr Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
title_full_unstemmed Cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (SBP): Application to the purification of SBP from toad
title_sort cyclic biospecific affinity chromatographic method for the purification of the sex steroid binding protein (sbp): application to the purification of sbp from toad
url http://hdl.handle.net/20.500.12110/paper_03784347_v415_nC_p297_SantaColoma
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AT garraffohm cyclicbiospecificaffinitychromatographicmethodforthepurificationofthesexsteroidbindingproteinsbpapplicationtothepurificationofsbpfromtoad
AT groseg cyclicbiospecificaffinitychromatographicmethodforthepurificationofthesexsteroidbindingproteinsbpapplicationtothepurificationofsbpfromtoad
AT charreaueh cyclicbiospecificaffinitychromatographicmethodforthepurificationofthesexsteroidbindingproteinsbpapplicationtothepurificationofsbpfromtoad
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