Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)

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Genus Helianthus comprises diploid and polyploid species. An autoallopolyploid origin has been proposed for hexaploid species but the genomic relationships remain unclear. Mitotic and meiotic studies in annual Helianthus annuus (2 n = 2 x = 34) and perennial Helianthus resinosus (2 n = 6 x = 102) as...

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Autores principales: Miranda Zanetti, J., Greizerstein, E., Camadro, E., Poverene, M., Echeverría, M., Poggio, L., Carrera, A.
Formato: INPR
Lenguaje:English
Materias:
2 n pollen
Allosyndesis
GISH
Interspecific hybrid
Polyploidy
Sunflower
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03782697_v_n_p1_MirandaZanetti
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_03782697_v_n_p1_MirandaZanetti2023-10-03T15:32:15Z Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae) Miranda Zanetti, J. Greizerstein, E. Camadro, E. Poverene, M. Echeverría, M. Poggio, L. Carrera, A. 2 n pollen Allosyndesis GISH Interspecific hybrid Polyploidy Sunflower Genus Helianthus comprises diploid and polyploid species. An autoallopolyploid origin has been proposed for hexaploid species but the genomic relationships remain unclear. Mitotic and meiotic studies in annual Helianthus annuus (2 n = 2 x = 34) and perennial Helianthus resinosus (2 n = 6 x = 102) as well as the F1 hybrids between both species were carried out. Chromosome counting confirmed the hybrid origin of the latter plants and their tetraploid condition. Bivalents in hybrids ranged from 12 to 28 ( {Mathematical expression} = 20.8). Univalents, trivalents and quadrivalents were also observed. Meiotic products comprised dyads, triads and normal tetrads and pollen grains were heterogeneous in size. These observations suggest the occurrence of 2 n pollen in addition to the expected n. Genomic in situ hybridization (GISH) of total H. annuus DNA on H. resinosus chromosomes rendered weak but uniform signals; similar hybridization pattern was observed using three other annual species. Hybridization with H. annuus probe performed on root tip cells of F1H. annuus × H. resinosus hybrids revealed 17 chromosomes with a strong hybridization signal. GISH in hybrid meiocytes distinguished chromosomes from parental species and revealed autosyndetic pairing of H. resinosus chromosomes, allosyndetic pairing in bivalents, trivalents and quadrivalents, and the presence of univalents derived from parents, H. annuus and H. resinosus. Results obtained from classical and molecular cytogenetics do not support H. annuus as a direct ancestor of H. resinosus. The occurrence of allosyndetic pairing and the relatively high fertility of the F1 hybrids point to the possibility that useful genes could be transferred from H. resinosus to cultivate sunflower, although the effective rate of recombination has not been evaluated. GISH method proved effective to recognize parental chromosomes in H. annuus × H. resinosus progeny. © 2013 Springer-Verlag Wien. Fil:Greizerstein, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. INPR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03782697_v_n_p1_MirandaZanetti
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language English
orig_language_str_mv English
topic 2 n pollen
Allosyndesis
GISH
Interspecific hybrid
Polyploidy
Sunflower
spellingShingle 2 n pollen
Allosyndesis
GISH
Interspecific hybrid
Polyploidy
Sunflower
Miranda Zanetti, J.
Greizerstein, E.
Camadro, E.
Poverene, M.
Echeverría, M.
Poggio, L.
Carrera, A.
Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)
topic_facet 2 n pollen
Allosyndesis
GISH
Interspecific hybrid
Polyploidy
Sunflower
description Genus Helianthus comprises diploid and polyploid species. An autoallopolyploid origin has been proposed for hexaploid species but the genomic relationships remain unclear. Mitotic and meiotic studies in annual Helianthus annuus (2 n = 2 x = 34) and perennial Helianthus resinosus (2 n = 6 x = 102) as well as the F1 hybrids between both species were carried out. Chromosome counting confirmed the hybrid origin of the latter plants and their tetraploid condition. Bivalents in hybrids ranged from 12 to 28 ( {Mathematical expression} = 20.8). Univalents, trivalents and quadrivalents were also observed. Meiotic products comprised dyads, triads and normal tetrads and pollen grains were heterogeneous in size. These observations suggest the occurrence of 2 n pollen in addition to the expected n. Genomic in situ hybridization (GISH) of total H. annuus DNA on H. resinosus chromosomes rendered weak but uniform signals; similar hybridization pattern was observed using three other annual species. Hybridization with H. annuus probe performed on root tip cells of F1H. annuus × H. resinosus hybrids revealed 17 chromosomes with a strong hybridization signal. GISH in hybrid meiocytes distinguished chromosomes from parental species and revealed autosyndetic pairing of H. resinosus chromosomes, allosyndetic pairing in bivalents, trivalents and quadrivalents, and the presence of univalents derived from parents, H. annuus and H. resinosus. Results obtained from classical and molecular cytogenetics do not support H. annuus as a direct ancestor of H. resinosus. The occurrence of allosyndetic pairing and the relatively high fertility of the F1 hybrids point to the possibility that useful genes could be transferred from H. resinosus to cultivate sunflower, although the effective rate of recombination has not been evaluated. GISH method proved effective to recognize parental chromosomes in H. annuus × H. resinosus progeny. © 2013 Springer-Verlag Wien.
format INPR
author Miranda Zanetti, J.
Greizerstein, E.
Camadro, E.
Poverene, M.
Echeverría, M.
Poggio, L.
Carrera, A.
author_facet Miranda Zanetti, J.
Greizerstein, E.
Camadro, E.
Poverene, M.
Echeverría, M.
Poggio, L.
Carrera, A.
author_sort Miranda Zanetti, J.
title Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)
title_short Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)
title_full Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)
title_fullStr Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)
title_full_unstemmed Genomic relationships between hexaploid Helianthus resinosus and diploid Helianthus annuus (Asteraceae)
title_sort genomic relationships between hexaploid helianthus resinosus and diploid helianthus annuus (asteraceae)
url http://hdl.handle.net/20.500.12110/paper_03782697_v_n_p1_MirandaZanetti
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