Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi
Cell-free extracts of epimastigotes of Trypanosoma cruzi contain tyrosine aminotransferase (TAT) and p-hydroxyphenyllactate dehydrogenase (pHPLDH). The TAT activity could be separated from aspartate aminotransferase (ASAT) by polyacrylamide gel electrophoresis or DEAE-cellulose chromatography; the l...
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todo:paper_03781097_v92_n2_p119_Nowicki2023-10-03T15:31:46Z Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi Nowicki, C. Montemartini, M. Duschak, V. Santomé, JosA. Cazzulo, J.J. p-Hydroxyphenyl lactate dehydrogenase Trypanosoma cruzi Tyrosine aminotransferase 4 hydroxyphenyllactic acid 4-hydroxyphenyllactic acid oxidoreductase phenylpropionic acid derivative tyrosine aminotransferase aspartate aminotransferase tyrosine aminotransferase animal article cell cycle cell fractionation enzymology isolation and purification metabolism Trypanosoma cruzi ultrastructure epimastigote mitochondrion nonhuman priority journal protein localization trypanosoma cruzi Animal Cell Cycle Oxidoreductases Phenylpropionates Subcellular Fractions Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase Trypanosoma Trypanosoma cruzi Cell-free extracts of epimastigotes of Trypanosoma cruzi contain tyrosine aminotransferase (TAT) and p-hydroxyphenyllactate dehydrogenase (pHPLDH). The TAT activity could be separated from aspartate aminotransferase (ASAT) by polyacrylamide gel electrophoresis or DEAE-cellulose chromatography; the latter procedure also allowed complete separation of pHPLDH. The subcellular localization of both T. cruzi enzymes, as determined by digitonin extraction, subcellular fractionation by differential centrifugation, and isopycnic ultracentrifugation in sucrose gradients, was mainly cytosolic, with low mitochondrial activities. © 1992. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v92_n2_p119_Nowicki |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
p-Hydroxyphenyl lactate dehydrogenase Trypanosoma cruzi Tyrosine aminotransferase 4 hydroxyphenyllactic acid 4-hydroxyphenyllactic acid oxidoreductase phenylpropionic acid derivative tyrosine aminotransferase aspartate aminotransferase tyrosine aminotransferase animal article cell cycle cell fractionation enzymology isolation and purification metabolism Trypanosoma cruzi ultrastructure epimastigote mitochondrion nonhuman priority journal protein localization trypanosoma cruzi Animal Cell Cycle Oxidoreductases Phenylpropionates Subcellular Fractions Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase Trypanosoma Trypanosoma cruzi |
| spellingShingle |
p-Hydroxyphenyl lactate dehydrogenase Trypanosoma cruzi Tyrosine aminotransferase 4 hydroxyphenyllactic acid 4-hydroxyphenyllactic acid oxidoreductase phenylpropionic acid derivative tyrosine aminotransferase aspartate aminotransferase tyrosine aminotransferase animal article cell cycle cell fractionation enzymology isolation and purification metabolism Trypanosoma cruzi ultrastructure epimastigote mitochondrion nonhuman priority journal protein localization trypanosoma cruzi Animal Cell Cycle Oxidoreductases Phenylpropionates Subcellular Fractions Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase Trypanosoma Trypanosoma cruzi Nowicki, C. Montemartini, M. Duschak, V. Santomé, JosA. Cazzulo, J.J. Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi |
| topic_facet |
p-Hydroxyphenyl lactate dehydrogenase Trypanosoma cruzi Tyrosine aminotransferase 4 hydroxyphenyllactic acid 4-hydroxyphenyllactic acid oxidoreductase phenylpropionic acid derivative tyrosine aminotransferase aspartate aminotransferase tyrosine aminotransferase animal article cell cycle cell fractionation enzymology isolation and purification metabolism Trypanosoma cruzi ultrastructure epimastigote mitochondrion nonhuman priority journal protein localization trypanosoma cruzi Animal Cell Cycle Oxidoreductases Phenylpropionates Subcellular Fractions Support, Non-U.S. Gov't Trypanosoma cruzi Tyrosine Transaminase Trypanosoma Trypanosoma cruzi |
| description |
Cell-free extracts of epimastigotes of Trypanosoma cruzi contain tyrosine aminotransferase (TAT) and p-hydroxyphenyllactate dehydrogenase (pHPLDH). The TAT activity could be separated from aspartate aminotransferase (ASAT) by polyacrylamide gel electrophoresis or DEAE-cellulose chromatography; the latter procedure also allowed complete separation of pHPLDH. The subcellular localization of both T. cruzi enzymes, as determined by digitonin extraction, subcellular fractionation by differential centrifugation, and isopycnic ultracentrifugation in sucrose gradients, was mainly cytosolic, with low mitochondrial activities. © 1992. |
| format |
JOUR |
| author |
Nowicki, C. Montemartini, M. Duschak, V. Santomé, JosA. Cazzulo, J.J. |
| author_facet |
Nowicki, C. Montemartini, M. Duschak, V. Santomé, JosA. Cazzulo, J.J. |
| author_sort |
Nowicki, C. |
| title |
Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi |
| title_short |
Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi |
| title_full |
Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi |
| title_fullStr |
Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi |
| title_full_unstemmed |
Presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of Trypanosoma cruzi |
| title_sort |
presence and subcellular localization of tyrosine aminotransferase and p-hydroxyphenyllactate dehydrogenase in epimastigotes of trypanosoma cruzi |
| url |
http://hdl.handle.net/20.500.12110/paper_03781097_v92_n2_p119_Nowicki |
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