A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi
Epinastigotes of different stocks of Trypanosoma cruzi contain levels of proteinase activity on azocasein; amastigotes and trypmastigotes contain 10-fold lower levels of this proteolytic activity, which seems, therefore, to be developmentally regulated. The proteinase could be detected as a broad ba...
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todo:paper_03781097_v67_n1-2_p145_Campetella2023-10-03T15:31:45Z A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi Campetella, O. Martínez, J. Cazzulo, J.J. Cysteine proteinases Polyacrylamide gels Trypanosoma cruzi Western blots cysteine proteinase cysteine proteinase article filaria nonhuman priority journal protozoon trypanosoma animal enzymology growth, development and aging isolation and purification metabolism molecular weight polyacrylamide gel electrophoresis Trypanosoma cruzi Western blotting Filaria Oryctolagus cuniculus Protozoa Trypanosoma Trypanosoma cruzi Animal Blotting, Western Cysteine Endopeptidases Electrophoresis, Polyacrylamide Gel Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi Epinastigotes of different stocks of Trypanosoma cruzi contain levels of proteinase activity on azocasein; amastigotes and trypmastigotes contain 10-fold lower levels of this proteolytic activity, which seems, therefore, to be developmentally regulated. The proteinase could be detected as a broad band, centered at about 60 kDa, which in some resolved into two close bands, in (a) SDS-polyacrylamide gels containing fibrinogen, and (b) Western blots probed with polyclonal rabbit antiserum prepared against purified cystein proteinase. No proteinase activity was observed at molecular weights lower than 55 kDa. The results show that the enzyme preciously purified is teh major cysteine proteinase present in epimastigotes of all stocks of T. cruzi tested. © 1990. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v67_n1-2_p145_Campetella |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cysteine proteinases Polyacrylamide gels Trypanosoma cruzi Western blots cysteine proteinase cysteine proteinase article filaria nonhuman priority journal protozoon trypanosoma animal enzymology growth, development and aging isolation and purification metabolism molecular weight polyacrylamide gel electrophoresis Trypanosoma cruzi Western blotting Filaria Oryctolagus cuniculus Protozoa Trypanosoma Trypanosoma cruzi Animal Blotting, Western Cysteine Endopeptidases Electrophoresis, Polyacrylamide Gel Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi |
| spellingShingle |
Cysteine proteinases Polyacrylamide gels Trypanosoma cruzi Western blots cysteine proteinase cysteine proteinase article filaria nonhuman priority journal protozoon trypanosoma animal enzymology growth, development and aging isolation and purification metabolism molecular weight polyacrylamide gel electrophoresis Trypanosoma cruzi Western blotting Filaria Oryctolagus cuniculus Protozoa Trypanosoma Trypanosoma cruzi Animal Blotting, Western Cysteine Endopeptidases Electrophoresis, Polyacrylamide Gel Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi Campetella, O. Martínez, J. Cazzulo, J.J. A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi |
| topic_facet |
Cysteine proteinases Polyacrylamide gels Trypanosoma cruzi Western blots cysteine proteinase cysteine proteinase article filaria nonhuman priority journal protozoon trypanosoma animal enzymology growth, development and aging isolation and purification metabolism molecular weight polyacrylamide gel electrophoresis Trypanosoma cruzi Western blotting Filaria Oryctolagus cuniculus Protozoa Trypanosoma Trypanosoma cruzi Animal Blotting, Western Cysteine Endopeptidases Electrophoresis, Polyacrylamide Gel Molecular Weight Support, Non-U.S. Gov't Trypanosoma cruzi |
| description |
Epinastigotes of different stocks of Trypanosoma cruzi contain levels of proteinase activity on azocasein; amastigotes and trypmastigotes contain 10-fold lower levels of this proteolytic activity, which seems, therefore, to be developmentally regulated. The proteinase could be detected as a broad band, centered at about 60 kDa, which in some resolved into two close bands, in (a) SDS-polyacrylamide gels containing fibrinogen, and (b) Western blots probed with polyclonal rabbit antiserum prepared against purified cystein proteinase. No proteinase activity was observed at molecular weights lower than 55 kDa. The results show that the enzyme preciously purified is teh major cysteine proteinase present in epimastigotes of all stocks of T. cruzi tested. © 1990. |
| format |
JOUR |
| author |
Campetella, O. Martínez, J. Cazzulo, J.J. |
| author_facet |
Campetella, O. Martínez, J. Cazzulo, J.J. |
| author_sort |
Campetella, O. |
| title |
A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi |
| title_short |
A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi |
| title_full |
A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi |
| title_fullStr |
A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi |
| title_full_unstemmed |
A major cysteine proteinase is developmentally regulated in Trypanosoma cruzi |
| title_sort |
major cysteine proteinase is developmentally regulated in trypanosoma cruzi |
| url |
http://hdl.handle.net/20.500.12110/paper_03781097_v67_n1-2_p145_Campetella |
| work_keys_str_mv |
AT campetellao amajorcysteineproteinaseisdevelopmentallyregulatedintrypanosomacruzi AT martinezj amajorcysteineproteinaseisdevelopmentallyregulatedintrypanosomacruzi AT cazzulojj amajorcysteineproteinaseisdevelopmentallyregulatedintrypanosomacruzi AT campetellao majorcysteineproteinaseisdevelopmentallyregulatedintrypanosomacruzi AT martinezj majorcysteineproteinaseisdevelopmentallyregulatedintrypanosomacruzi AT cazzulojj majorcysteineproteinaseisdevelopmentallyregulatedintrypanosomacruzi |
| _version_ |
1807319446365143040 |