Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum

Little is known about isoprenoid biosynthesis in parasitic protozoa. The presence of dolichol and isoprenylated proteins has been detected in Plasmodium falciparum, but no studies are available about the biosynthesis of the isoprenic side chain attached to the benzoquinone ring of coenzyme Q. In the...

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Detalles Bibliográficos
Autores principales: De Macedo, C.S., Uhrig, M.L., Kimura, E.A., Katzin, A.M.
Formato: JOUR
Materias:
Apicomplexan
Coenzyme Q
Isoprenoid
Malaria
Plasmodium falciparum
isoprenoid
nerolidol
ubiquinone
antimalarial activity
article
biosynthesis
controlled study
drug mechanism
erythrocyte
IC 50
nonhuman
parasite development
priority journal
Animals
Carbon Radioisotopes
Erythrocytes
Humans
Malaria, Falciparum
Parasitic Sensitivity Tests
Polyisoprenyl Phosphates
Protein Isoprenylation
Sesquiterpenes
Tritium
Ubiquinone
Protozoa
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03781097_v207_n1_p13_DeMacedo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03781097_v207_n1_p13_DeMacedo
record_format dspace
spelling todo:paper_03781097_v207_n1_p13_DeMacedo2023-10-03T15:31:41Z Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum De Macedo, C.S. Uhrig, M.L. Kimura, E.A. Katzin, A.M. Apicomplexan Coenzyme Q Isoprenoid Malaria Plasmodium falciparum isoprenoid nerolidol ubiquinone antimalarial activity article biosynthesis controlled study drug mechanism erythrocyte IC 50 nonhuman parasite development Plasmodium falciparum priority journal Animals Carbon Radioisotopes Erythrocytes Humans Malaria, Falciparum Parasitic Sensitivity Tests Plasmodium falciparum Polyisoprenyl Phosphates Protein Isoprenylation Sesquiterpenes Tritium Ubiquinone Plasmodium falciparum Protozoa Little is known about isoprenoid biosynthesis in parasitic protozoa. The presence of dolichol and isoprenylated proteins has been detected in Plasmodium falciparum, but no studies are available about the biosynthesis of the isoprenic side chain attached to the benzoquinone ring of coenzyme Q. In the present study, using metabolic labelling with different intermediates, we demonstrated the presence of an active isoprenoid pathway for the biosynthesis of the isoprenic chain of coenzyme Q. Our results show that P. falciparum is able to synthesize different homologs (coenzyme Q8 and coenzyme Q9), depending on the given intermediate. Parasites treated with nerolidol at doses 2.2 times below the IC50 showed a decreased ability to synthesize the isoprenic chain attached to coenzyme Q at all intraerythrocytic stages. Treatment with nerolidol arrested development of the intraerythrocytic stages of the parasites, indicating that the drug may have an antimalarial potential. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v207_n1_p13_DeMacedo
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Apicomplexan
Coenzyme Q
Isoprenoid
Malaria
Plasmodium falciparum
isoprenoid
nerolidol
ubiquinone
antimalarial activity
article
biosynthesis
controlled study
drug mechanism
erythrocyte
IC 50
nonhuman
parasite development
Plasmodium falciparum
priority journal
Animals
Carbon Radioisotopes
Erythrocytes
Humans
Malaria, Falciparum
Parasitic Sensitivity Tests
Plasmodium falciparum
Polyisoprenyl Phosphates
Protein Isoprenylation
Sesquiterpenes
Tritium
Ubiquinone
Plasmodium falciparum
Protozoa
spellingShingle Apicomplexan
Coenzyme Q
Isoprenoid
Malaria
Plasmodium falciparum
isoprenoid
nerolidol
ubiquinone
antimalarial activity
article
biosynthesis
controlled study
drug mechanism
erythrocyte
IC 50
nonhuman
parasite development
Plasmodium falciparum
priority journal
Animals
Carbon Radioisotopes
Erythrocytes
Humans
Malaria, Falciparum
Parasitic Sensitivity Tests
Plasmodium falciparum
Polyisoprenyl Phosphates
Protein Isoprenylation
Sesquiterpenes
Tritium
Ubiquinone
Plasmodium falciparum
Protozoa
De Macedo, C.S.
Uhrig, M.L.
Kimura, E.A.
Katzin, A.M.
Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum
topic_facet Apicomplexan
Coenzyme Q
Isoprenoid
Malaria
Plasmodium falciparum
isoprenoid
nerolidol
ubiquinone
antimalarial activity
article
biosynthesis
controlled study
drug mechanism
erythrocyte
IC 50
nonhuman
parasite development
Plasmodium falciparum
priority journal
Animals
Carbon Radioisotopes
Erythrocytes
Humans
Malaria, Falciparum
Parasitic Sensitivity Tests
Plasmodium falciparum
Polyisoprenyl Phosphates
Protein Isoprenylation
Sesquiterpenes
Tritium
Ubiquinone
Plasmodium falciparum
Protozoa
description Little is known about isoprenoid biosynthesis in parasitic protozoa. The presence of dolichol and isoprenylated proteins has been detected in Plasmodium falciparum, but no studies are available about the biosynthesis of the isoprenic side chain attached to the benzoquinone ring of coenzyme Q. In the present study, using metabolic labelling with different intermediates, we demonstrated the presence of an active isoprenoid pathway for the biosynthesis of the isoprenic chain of coenzyme Q. Our results show that P. falciparum is able to synthesize different homologs (coenzyme Q8 and coenzyme Q9), depending on the given intermediate. Parasites treated with nerolidol at doses 2.2 times below the IC50 showed a decreased ability to synthesize the isoprenic chain attached to coenzyme Q at all intraerythrocytic stages. Treatment with nerolidol arrested development of the intraerythrocytic stages of the parasites, indicating that the drug may have an antimalarial potential. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
format JOUR
author De Macedo, C.S.
Uhrig, M.L.
Kimura, E.A.
Katzin, A.M.
author_facet De Macedo, C.S.
Uhrig, M.L.
Kimura, E.A.
Katzin, A.M.
author_sort De Macedo, C.S.
title Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum
title_short Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum
title_full Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum
title_fullStr Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum
title_full_unstemmed Characterization of the isoprenoid chain of coenzyme Q in Plasmodium falciparum
title_sort characterization of the isoprenoid chain of coenzyme q in plasmodium falciparum
url http://hdl.handle.net/20.500.12110/paper_03781097_v207_n1_p13_DeMacedo
work_keys_str_mv AT demacedocs characterizationoftheisoprenoidchainofcoenzymeqinplasmodiumfalciparum
AT uhrigml characterizationoftheisoprenoidchainofcoenzymeqinplasmodiumfalciparum
AT kimuraea characterizationoftheisoprenoidchainofcoenzymeqinplasmodiumfalciparum
AT katzinam characterizationoftheisoprenoidchainofcoenzymeqinplasmodiumfalciparum
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