The spoOA and degU genes of Bacillus subtilis show genetic homology

The transformation efficiency of competent Bacillus subtilis degU32(Hy) strains was found to depend on the marker that was selected. Protrophic transformants were obtained at frequencies similar to those in the wild type control, but Spo - transformants oere rare also when a spoOA::erm insertion tha...

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Autores principales: Cucchi, A., Sanchez-Rivas, C.
Formato: JOUR
Materias:
Bacillus subtilis
Exo-enzymes
spoOA, degU
Sporulation control genes
Transformation efficiency
article
bacillus subtilis
genetic transformation
nonhuman
priority journal
sequence homology
sporogenesis
Chromosome Mapping
DNA Transposable Elements
Genes, Bacterial
Genetic Markers
Mutation
Spores, Bacterial
Support, Non-U.S. Gov't
Transduction, Genetic
Transformation, Genetic
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03781097_v107_n2-3_p307_Cucchi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03781097_v107_n2-3_p307_Cucchi
record_format dspace
spelling todo:paper_03781097_v107_n2-3_p307_Cucchi2023-10-03T15:31:37Z The spoOA and degU genes of Bacillus subtilis show genetic homology Cucchi, A. Sanchez-Rivas, C. Bacillus subtilis Exo-enzymes spoOA, degU Sporulation control genes Transformation efficiency article bacillus subtilis genetic transformation nonhuman priority journal sequence homology sporogenesis Bacillus subtilis Chromosome Mapping DNA Transposable Elements Genes, Bacterial Genetic Markers Mutation Spores, Bacterial Support, Non-U.S. Gov't Transduction, Genetic Transformation, Genetic The transformation efficiency of competent Bacillus subtilis degU32(Hy) strains was found to depend on the marker that was selected. Protrophic transformants were obtained at frequencies similar to those in the wild type control, but Spo - transformants oere rare also when a spoOA::erm insertion that produces a selectable marker (Erm R ) was used. The Erm R transformants obtained within the degU32(Hy) background were Spo + and had lost the characteristics of the DegU(Hy) parental recipient strain i.e., secretion of exo-enzymes and sporulation resistance to catabolites. The spoOA::erm insertion was mapped to a location near degU. The similarities between the spoOA and degU sequences and the metabolic interferences between the mutated products which result in this unexpected recombination, are discussed. © 1993. Fil:Cucchi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03781097_v107_n2-3_p307_Cucchi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Bacillus subtilis
Exo-enzymes
spoOA, degU
Sporulation control genes
Transformation efficiency
article
bacillus subtilis
genetic transformation
nonhuman
priority journal
sequence homology
sporogenesis
Bacillus subtilis
Chromosome Mapping
DNA Transposable Elements
Genes, Bacterial
Genetic Markers
Mutation
Spores, Bacterial
Support, Non-U.S. Gov't
Transduction, Genetic
Transformation, Genetic
spellingShingle Bacillus subtilis
Exo-enzymes
spoOA, degU
Sporulation control genes
Transformation efficiency
article
bacillus subtilis
genetic transformation
nonhuman
priority journal
sequence homology
sporogenesis
Bacillus subtilis
Chromosome Mapping
DNA Transposable Elements
Genes, Bacterial
Genetic Markers
Mutation
Spores, Bacterial
Support, Non-U.S. Gov't
Transduction, Genetic
Transformation, Genetic
Cucchi, A.
Sanchez-Rivas, C.
The spoOA and degU genes of Bacillus subtilis show genetic homology
topic_facet Bacillus subtilis
Exo-enzymes
spoOA, degU
Sporulation control genes
Transformation efficiency
article
bacillus subtilis
genetic transformation
nonhuman
priority journal
sequence homology
sporogenesis
Bacillus subtilis
Chromosome Mapping
DNA Transposable Elements
Genes, Bacterial
Genetic Markers
Mutation
Spores, Bacterial
Support, Non-U.S. Gov't
Transduction, Genetic
Transformation, Genetic
description The transformation efficiency of competent Bacillus subtilis degU32(Hy) strains was found to depend on the marker that was selected. Protrophic transformants were obtained at frequencies similar to those in the wild type control, but Spo - transformants oere rare also when a spoOA::erm insertion that produces a selectable marker (Erm R ) was used. The Erm R transformants obtained within the degU32(Hy) background were Spo + and had lost the characteristics of the DegU(Hy) parental recipient strain i.e., secretion of exo-enzymes and sporulation resistance to catabolites. The spoOA::erm insertion was mapped to a location near degU. The similarities between the spoOA and degU sequences and the metabolic interferences between the mutated products which result in this unexpected recombination, are discussed. © 1993.
format JOUR
author Cucchi, A.
Sanchez-Rivas, C.
author_facet Cucchi, A.
Sanchez-Rivas, C.
author_sort Cucchi, A.
title The spoOA and degU genes of Bacillus subtilis show genetic homology
title_short The spoOA and degU genes of Bacillus subtilis show genetic homology
title_full The spoOA and degU genes of Bacillus subtilis show genetic homology
title_fullStr The spoOA and degU genes of Bacillus subtilis show genetic homology
title_full_unstemmed The spoOA and degU genes of Bacillus subtilis show genetic homology
title_sort spooa and degu genes of bacillus subtilis show genetic homology
url http://hdl.handle.net/20.500.12110/paper_03781097_v107_n2-3_p307_Cucchi
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AT sanchezrivasc thespooaanddegugenesofbacillussubtilisshowgenetichomology
AT cucchia spooaanddegugenesofbacillussubtilisshowgenetichomology
AT sanchezrivasc spooaanddegugenesofbacillussubtilisshowgenetichomology
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