The inhibitory action of aluminum on mouse bone marrow cell growth: Evidence for an erythropoietin- and transferrin-mediated mechanism

Aluminum (Al) has been associated with anemia in chronic renal failure patients under hemodialysis as well as in Al-overloaded animals. In an attempt to elucidate further the mechanism of Al toxicity we have investigated the effect of this ion on erythropoiesis in vitro. Mouse bone marrow cells were...

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Autores principales:	Garbossa, G., Gutnisky, A., Nesse, A.
Formato:	JOUR
Materias:	aluminum aluminum toxicity anemia erythroid colony-forming units erythropoiesis erythropoietin transferrin animal cell article bone marrow cell culture cell growth cell metabolism mouse nonhuman priority journal Aluminum Animal Bone Marrow Bone Marrow Cells Cell Division Cells, Cultured Erythroid Progenitor Cells Erythropoiesis Erythropoietin Mice Support, Non-U.S. Gov't Transferrin
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03780392_v20_n3_p141_Garbossa
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03780392_v20_n3_p141_Garbossa
record_format	dspace
spelling	todo:paper_03780392_v20_n3_p141_Garbossa2023-10-03T15:31:37Z The inhibitory action of aluminum on mouse bone marrow cell growth: Evidence for an erythropoietin- and transferrin-mediated mechanism Garbossa, G. Gutnisky, A. Nesse, A. aluminum aluminum toxicity anemia erythroid colony-forming units erythropoiesis aluminum erythropoietin transferrin animal cell article bone marrow cell culture cell growth cell metabolism erythropoiesis mouse nonhuman priority journal Aluminum Animal Bone Marrow Bone Marrow Cells Cell Division Cells, Cultured Erythroid Progenitor Cells Erythropoiesis Erythropoietin Mice Support, Non-U.S. Gov't Transferrin Aluminum (Al) has been associated with anemia in chronic renal failure patients under hemodialysis as well as in Al-overloaded animals. In an attempt to elucidate further the mechanism of Al toxicity we have investigated the effect of this ion on erythropoiesis in vitro. Mouse bone marrow cells were stimulated in vitro with erythropoietin (Epo) in the presence of Al3+ ion and erythroid colony-forming units were then determined. Results of this study indicate that Al compounds (chloride and citrate) at concentrations as low as 0.37 μmol Al/l inhibit erythropoiesis in vitro through a mechanism dependent upon the availability of transferrin to bind to aluminum. This process cannot be reversed by increasing Epo doses. This inhibition only occurs in the presence of Epo at early stages during the interaction of the hormone with its target cell. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03780392_v20_n3_p141_Garbossa
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	aluminum aluminum toxicity anemia erythroid colony-forming units erythropoiesis aluminum erythropoietin transferrin animal cell article bone marrow cell culture cell growth cell metabolism erythropoiesis mouse nonhuman priority journal Aluminum Animal Bone Marrow Bone Marrow Cells Cell Division Cells, Cultured Erythroid Progenitor Cells Erythropoiesis Erythropoietin Mice Support, Non-U.S. Gov't Transferrin
spellingShingle	aluminum aluminum toxicity anemia erythroid colony-forming units erythropoiesis aluminum erythropoietin transferrin animal cell article bone marrow cell culture cell growth cell metabolism erythropoiesis mouse nonhuman priority journal Aluminum Animal Bone Marrow Bone Marrow Cells Cell Division Cells, Cultured Erythroid Progenitor Cells Erythropoiesis Erythropoietin Mice Support, Non-U.S. Gov't Transferrin Garbossa, G. Gutnisky, A. Nesse, A. The inhibitory action of aluminum on mouse bone marrow cell growth: Evidence for an erythropoietin- and transferrin-mediated mechanism
topic_facet	aluminum aluminum toxicity anemia erythroid colony-forming units erythropoiesis aluminum erythropoietin transferrin animal cell article bone marrow cell culture cell growth cell metabolism erythropoiesis mouse nonhuman priority journal Aluminum Animal Bone Marrow Bone Marrow Cells Cell Division Cells, Cultured Erythroid Progenitor Cells Erythropoiesis Erythropoietin Mice Support, Non-U.S. Gov't Transferrin
description	Aluminum (Al) has been associated with anemia in chronic renal failure patients under hemodialysis as well as in Al-overloaded animals. In an attempt to elucidate further the mechanism of Al toxicity we have investigated the effect of this ion on erythropoiesis in vitro. Mouse bone marrow cells were stimulated in vitro with erythropoietin (Epo) in the presence of Al3+ ion and erythroid colony-forming units were then determined. Results of this study indicate that Al compounds (chloride and citrate) at concentrations as low as 0.37 μmol Al/l inhibit erythropoiesis in vitro through a mechanism dependent upon the availability of transferrin to bind to aluminum. This process cannot be reversed by increasing Epo doses. This inhibition only occurs in the presence of Epo at early stages during the interaction of the hormone with its target cell.
format	JOUR
author	Garbossa, G. Gutnisky, A. Nesse, A.
author_facet	Garbossa, G. Gutnisky, A. Nesse, A.
author_sort	Garbossa, G.
title	The inhibitory action of aluminum on mouse bone marrow cell growth: Evidence for an erythropoietin- and transferrin-mediated mechanism
title_short	The inhibitory action of aluminum on mouse bone marrow cell growth: Evidence for an erythropoietin- and transferrin-mediated mechanism
title_full	The inhibitory action of aluminum on mouse bone marrow cell growth: Evidence for an erythropoietin- and transferrin-mediated mechanism
title_fullStr	The inhibitory action of aluminum on mouse bone marrow cell growth: Evidence for an erythropoietin- and transferrin-mediated mechanism
title_full_unstemmed	The inhibitory action of aluminum on mouse bone marrow cell growth: Evidence for an erythropoietin- and transferrin-mediated mechanism
title_sort	inhibitory action of aluminum on mouse bone marrow cell growth: evidence for an erythropoietin- and transferrin-mediated mechanism
url	http://hdl.handle.net/20.500.12110/paper_03780392_v20_n3_p141_Garbossa
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The inhibitory action of aluminum on mouse bone marrow cell growth: Evidence for an erythropoietin- and transferrin-mediated mechanism

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