Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS
Bile acids, bile salts and aminoacids are important tracers of different newborn pathologies. Their detection and quantification by simple and secure methods are very important, specially if they can be carried out on the original biological fluid, avoiding previous isolation. In this sense, tandem...
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todo:paper_03650375_v86_n3-6_p110_Seldes2023-10-03T15:27:49Z Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS Seldes, A.M. Fernandez Bieber, G. Bile acids, bile salts and aminoacids are important tracers of different newborn pathologies. Their detection and quantification by simple and secure methods are very important, specially if they can be carried out on the original biological fluid, avoiding previous isolation. In this sense, tandem mass spectrometry is the method of choice. In order to improve the level of detection of these type of compounds when using negative FAB-MS-MS we tried different concentrations of each of them in four different matrixes: triethanolamine, glycerol, glycerol + NH4OH [1:2] and glycerol + NH4OH [2:1]. The results allowed the construction of the sensitivity curves and the calculation of the respective detection limits (LOD and LOQ) for each of the samples in each of the different matrixes. The use of glycerol + NH4OH [1:2] improved greatly the detection level for all of them and resulted in a simple and effective option for the proposed study. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03650375_v86_n3-6_p110_Seldes |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| description |
Bile acids, bile salts and aminoacids are important tracers of different newborn pathologies. Their detection and quantification by simple and secure methods are very important, specially if they can be carried out on the original biological fluid, avoiding previous isolation. In this sense, tandem mass spectrometry is the method of choice. In order to improve the level of detection of these type of compounds when using negative FAB-MS-MS we tried different concentrations of each of them in four different matrixes: triethanolamine, glycerol, glycerol + NH4OH [1:2] and glycerol + NH4OH [2:1]. The results allowed the construction of the sensitivity curves and the calculation of the respective detection limits (LOD and LOQ) for each of the samples in each of the different matrixes. The use of glycerol + NH4OH [1:2] improved greatly the detection level for all of them and resulted in a simple and effective option for the proposed study. |
| format |
JOUR |
| author |
Seldes, A.M. Fernandez Bieber, G. |
| spellingShingle |
Seldes, A.M. Fernandez Bieber, G. Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS |
| author_facet |
Seldes, A.M. Fernandez Bieber, G. |
| author_sort |
Seldes, A.M. |
| title |
Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS |
| title_short |
Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS |
| title_full |
Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS |
| title_fullStr |
Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS |
| title_full_unstemmed |
Improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative FAB-MS-MS |
| title_sort |
improvement in the detection levels of bile acids, bile salts and aminoacids in the analysis by negative fab-ms-ms |
| url |
http://hdl.handle.net/20.500.12110/paper_03650375_v86_n3-6_p110_Seldes |
| work_keys_str_mv |
AT seldesam improvementinthedetectionlevelsofbileacidsbilesaltsandaminoacidsintheanalysisbynegativefabmsms AT fernandezbieberg improvementinthedetectionlevelsofbileacidsbilesaltsandaminoacidsintheanalysisbynegativefabmsms |
| _version_ |
1782028478889066496 |