Cholinergic and glutamatergic agonists induce gamma frequency activity in dorsal subcoeruleus nucleus neurons
The dorsal subcoeruleus nucleus (SubCD) is involved in generating two signs of rapid eye movement (REM) sleep: muscle atonia and ponto-geniculo-occipital (PGO) waves. We tested the hypothesis that single cell and/or population responses of SubCD neurons are capable of generating gamma frequency acti...
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todo:paper_03636143_v301_n2_pC327_Simon2023-10-03T15:27:32Z Cholinergic and glutamatergic agonists induce gamma frequency activity in dorsal subcoeruleus nucleus neurons Simon, C. Kezunovic, N. Keith Williams, D. Urbano, F.J. Garcia-Rill, E. Arousal Carbachol Kainic acid N-methyl-D-aspartic acid Rapid eye movement sleep carbachol cholinergic receptor cholinergic receptor stimulating agent glutamate receptor glutamate receptor agonist kainic acid lidocaine ethobromide n methyl dextro aspartic acid sodium channel tetrodotoxin action potential animal experiment animal tissue article brain nerve cell cell size gamma rhythm immunocytochemistry locus ceruleus membrane potential nonhuman oscillation patch clamp priority journal rat Action Potentials Analysis of Variance Animals Brain Waves Cholinergic Agonists Excitatory Amino Acid Agonists Kinetics Linear Models Neurons Oscillometry Patch-Clamp Techniques Pons Rats Rats, Sprague-Dawley Sleep, REM Sodium Channel Blockers Sodium Channels Rattus The dorsal subcoeruleus nucleus (SubCD) is involved in generating two signs of rapid eye movement (REM) sleep: muscle atonia and ponto-geniculo-occipital (PGO) waves. We tested the hypothesis that single cell and/or population responses of SubCD neurons are capable of generating gamma frequency activity in response to intracellular stimulation or receptor agonist activation. Whole cell patch clamp recordings (immersion chamber) and population responses (interface chamber) were conducted on 9- to 20-day-old rat brain stem slices. All SubCD neurons (n = 103) fired at gamma frequency when subjected to depolarizing steps. Two statistically distinct populations of neurons were observed, which were distinguished by their high (>80 Hz, n = 24) versus low (35-80 Hz, n = 16) initial firing frequencies. Both cell types exhibited subthreshold oscillations in the gamma range (n = 43), which may underlie the gamma band firing properties of these neurons. The subthreshold oscillations were blocked by the sodium channel blockers tetrodotoxin (TTX, n = 21) extracellularly and N-(2,6-dimethylphenylcarbamoylmethyl) triethylammonium bromide (QX-314) intracellularly (n = 5), indicating they were sodium channel dependent. Gamma frequency subthreshold oscillations were observed in response to the nonspecific cholinergic receptor agonist carbachol (CAR, n = 11, d = 1.08) and the glutamate receptor agonists N-methyl-D-aspartic acid (NMDA, n = 12, d = 1.09) and kainic acid (KA, n = 13, d = 0.96), indicating that cholinergic and glutamatergic inputs may be involved in the activation of these subthreshold currents. Gamma band activity also was observed in population responses following application of CAR (n = 4, P <0.05), NMDA (n = 4, P < 0.05) and KA (n = 4, P < 0.05). Voltage-sensitive, sodium channel-dependent gamma band activity appears to be a part of the intrinsic membrane properties of SubCD lneurons. © 2011 the American Physiological Society. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03636143_v301_n2_pC327_Simon |
| institution |
Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arousal Carbachol Kainic acid N-methyl-D-aspartic acid Rapid eye movement sleep carbachol cholinergic receptor cholinergic receptor stimulating agent glutamate receptor glutamate receptor agonist kainic acid lidocaine ethobromide n methyl dextro aspartic acid sodium channel tetrodotoxin action potential animal experiment animal tissue article brain nerve cell cell size gamma rhythm immunocytochemistry locus ceruleus membrane potential nonhuman oscillation patch clamp priority journal rat Action Potentials Analysis of Variance Animals Brain Waves Cholinergic Agonists Excitatory Amino Acid Agonists Kinetics Linear Models Neurons Oscillometry Patch-Clamp Techniques Pons Rats Rats, Sprague-Dawley Sleep, REM Sodium Channel Blockers Sodium Channels Rattus |
| spellingShingle |
Arousal Carbachol Kainic acid N-methyl-D-aspartic acid Rapid eye movement sleep carbachol cholinergic receptor cholinergic receptor stimulating agent glutamate receptor glutamate receptor agonist kainic acid lidocaine ethobromide n methyl dextro aspartic acid sodium channel tetrodotoxin action potential animal experiment animal tissue article brain nerve cell cell size gamma rhythm immunocytochemistry locus ceruleus membrane potential nonhuman oscillation patch clamp priority journal rat Action Potentials Analysis of Variance Animals Brain Waves Cholinergic Agonists Excitatory Amino Acid Agonists Kinetics Linear Models Neurons Oscillometry Patch-Clamp Techniques Pons Rats Rats, Sprague-Dawley Sleep, REM Sodium Channel Blockers Sodium Channels Rattus Simon, C. Kezunovic, N. Keith Williams, D. Urbano, F.J. Garcia-Rill, E. Cholinergic and glutamatergic agonists induce gamma frequency activity in dorsal subcoeruleus nucleus neurons |
| topic_facet |
Arousal Carbachol Kainic acid N-methyl-D-aspartic acid Rapid eye movement sleep carbachol cholinergic receptor cholinergic receptor stimulating agent glutamate receptor glutamate receptor agonist kainic acid lidocaine ethobromide n methyl dextro aspartic acid sodium channel tetrodotoxin action potential animal experiment animal tissue article brain nerve cell cell size gamma rhythm immunocytochemistry locus ceruleus membrane potential nonhuman oscillation patch clamp priority journal rat Action Potentials Analysis of Variance Animals Brain Waves Cholinergic Agonists Excitatory Amino Acid Agonists Kinetics Linear Models Neurons Oscillometry Patch-Clamp Techniques Pons Rats Rats, Sprague-Dawley Sleep, REM Sodium Channel Blockers Sodium Channels Rattus |
| description |
The dorsal subcoeruleus nucleus (SubCD) is involved in generating two signs of rapid eye movement (REM) sleep: muscle atonia and ponto-geniculo-occipital (PGO) waves. We tested the hypothesis that single cell and/or population responses of SubCD neurons are capable of generating gamma frequency activity in response to intracellular stimulation or receptor agonist activation. Whole cell patch clamp recordings (immersion chamber) and population responses (interface chamber) were conducted on 9- to 20-day-old rat brain stem slices. All SubCD neurons (n = 103) fired at gamma frequency when subjected to depolarizing steps. Two statistically distinct populations of neurons were observed, which were distinguished by their high (>80 Hz, n = 24) versus low (35-80 Hz, n = 16) initial firing frequencies. Both cell types exhibited subthreshold oscillations in the gamma range (n = 43), which may underlie the gamma band firing properties of these neurons. The subthreshold oscillations were blocked by the sodium channel blockers tetrodotoxin (TTX, n = 21) extracellularly and N-(2,6-dimethylphenylcarbamoylmethyl) triethylammonium bromide (QX-314) intracellularly (n = 5), indicating they were sodium channel dependent. Gamma frequency subthreshold oscillations were observed in response to the nonspecific cholinergic receptor agonist carbachol (CAR, n = 11, d = 1.08) and the glutamate receptor agonists N-methyl-D-aspartic acid (NMDA, n = 12, d = 1.09) and kainic acid (KA, n = 13, d = 0.96), indicating that cholinergic and glutamatergic inputs may be involved in the activation of these subthreshold currents. Gamma band activity also was observed in population responses following application of CAR (n = 4, P <0.05), NMDA (n = 4, P < 0.05) and KA (n = 4, P < 0.05). Voltage-sensitive, sodium channel-dependent gamma band activity appears to be a part of the intrinsic membrane properties of SubCD lneurons. © 2011 the American Physiological Society. |
| format |
JOUR |
| author |
Simon, C. Kezunovic, N. Keith Williams, D. Urbano, F.J. Garcia-Rill, E. |
| author_facet |
Simon, C. Kezunovic, N. Keith Williams, D. Urbano, F.J. Garcia-Rill, E. |
| author_sort |
Simon, C. |
| title |
Cholinergic and glutamatergic agonists induce gamma frequency activity in dorsal subcoeruleus nucleus neurons |
| title_short |
Cholinergic and glutamatergic agonists induce gamma frequency activity in dorsal subcoeruleus nucleus neurons |
| title_full |
Cholinergic and glutamatergic agonists induce gamma frequency activity in dorsal subcoeruleus nucleus neurons |
| title_fullStr |
Cholinergic and glutamatergic agonists induce gamma frequency activity in dorsal subcoeruleus nucleus neurons |
| title_full_unstemmed |
Cholinergic and glutamatergic agonists induce gamma frequency activity in dorsal subcoeruleus nucleus neurons |
| title_sort |
cholinergic and glutamatergic agonists induce gamma frequency activity in dorsal subcoeruleus nucleus neurons |
| url |
http://hdl.handle.net/20.500.12110/paper_03636143_v301_n2_pC327_Simon |
| work_keys_str_mv |
AT simonc cholinergicandglutamatergicagonistsinducegammafrequencyactivityindorsalsubcoeruleusnucleusneurons AT kezunovicn cholinergicandglutamatergicagonistsinducegammafrequencyactivityindorsalsubcoeruleusnucleusneurons AT keithwilliamsd cholinergicandglutamatergicagonistsinducegammafrequencyactivityindorsalsubcoeruleusnucleusneurons AT urbanofj cholinergicandglutamatergicagonistsinducegammafrequencyactivityindorsalsubcoeruleusnucleusneurons AT garciarille cholinergicandglutamatergicagonistsinducegammafrequencyactivityindorsalsubcoeruleusnucleusneurons |
| _version_ |
1807324244546158592 |