In vitro and in vivo evaluation of proximal tubular acidification in aging rats

The normal aging process is accompanied by a progressive deterioration of renal function. We studied the kinetics of proximal tubular acidification of young (3 mo) and aging (22 mo) rats using in vivo and in vitro techniques. Blood acid-base parameters were similar in both groups. The maximum veloci...

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Autores principales: Mac Laughlin, M., Damasco, M.C., Igarreta, P., Amorena, C.
Formato: JOUR
Materias:
Micropuncture
Na+/H+
Vesicles
acidification
aging
animal cell
animal experiment
animal tissue
article
blood pH
brush border
brush border vesicle
controlled study
disease course
kidney function
kidney proximal tubule
male
Michaelis constant
nonhuman
priority journal
proton transport
punch biopsy
rat
Acids
Aging
Animals
Bicarbonates
Blotting, Western
Hydrogen-Ion Concentration
Kidney Tubules, Proximal
Kinetics
Male
Microvilli
Osmolar Concentration
Punctures
Rats
Rats, Wistar
Sodium-Hydrogen Antiporter
Time Factors
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03636119_v280_n649-6_pR1627_MacLaughlin
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03636119_v280_n649-6_pR1627_MacLaughlin
record_format dspace
spelling todo:paper_03636119_v280_n649-6_pR1627_MacLaughlin2023-10-03T15:27:29Z In vitro and in vivo evaluation of proximal tubular acidification in aging rats Mac Laughlin, M. Damasco, M.C. Igarreta, P. Amorena, C. Micropuncture Na+/H+ Vesicles acidification aging animal cell animal experiment animal tissue article blood pH brush border brush border vesicle controlled study disease course kidney function kidney proximal tubule male Michaelis constant nonhuman priority journal proton transport punch biopsy rat Acids Aging Animals Bicarbonates Blotting, Western Hydrogen-Ion Concentration Kidney Tubules, Proximal Kinetics Male Microvilli Osmolar Concentration Punctures Rats Rats, Wistar Sodium-Hydrogen Antiporter Time Factors The normal aging process is accompanied by a progressive deterioration of renal function. We studied the kinetics of proximal tubular acidification of young (3 mo) and aging (22 mo) rats using in vivo and in vitro techniques. Blood acid-base parameters were similar in both groups. The maximum velocity of the Na+/H+ exchange (NHE) in brush-border membrane vesicles (BBMV) showed a 72% decrease in aging compared with young rats, whereas the Michaelis constant remained unchanged. The NHE3 isoform of the Na+/H+ exchanger was detected in BBMV by Western blot in both groups, and a decrease of 90% in the abundance was observed in aging rats. Micropuncture experiments with simultaneous luminal and peritubular perfusion with phosphate Ringer and continuous measurement of intratubular pH showed an acidification rate constant 34% smaller in aging compared with young rats. Proton flux was 48% lower in aging than in young rats. The present results suggest that proximal tubular acidification is impaired with aging. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03636119_v280_n649-6_pR1627_MacLaughlin
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Micropuncture
Na+/H+
Vesicles
acidification
aging
animal cell
animal experiment
animal tissue
article
blood pH
brush border
brush border vesicle
controlled study
disease course
kidney function
kidney proximal tubule
male
Michaelis constant
nonhuman
priority journal
proton transport
punch biopsy
rat
Acids
Aging
Animals
Bicarbonates
Blotting, Western
Hydrogen-Ion Concentration
Kidney Tubules, Proximal
Kinetics
Male
Microvilli
Osmolar Concentration
Punctures
Rats
Rats, Wistar
Sodium-Hydrogen Antiporter
Time Factors
spellingShingle Micropuncture
Na+/H+
Vesicles
acidification
aging
animal cell
animal experiment
animal tissue
article
blood pH
brush border
brush border vesicle
controlled study
disease course
kidney function
kidney proximal tubule
male
Michaelis constant
nonhuman
priority journal
proton transport
punch biopsy
rat
Acids
Aging
Animals
Bicarbonates
Blotting, Western
Hydrogen-Ion Concentration
Kidney Tubules, Proximal
Kinetics
Male
Microvilli
Osmolar Concentration
Punctures
Rats
Rats, Wistar
Sodium-Hydrogen Antiporter
Time Factors
Mac Laughlin, M.
Damasco, M.C.
Igarreta, P.
Amorena, C.
In vitro and in vivo evaluation of proximal tubular acidification in aging rats
topic_facet Micropuncture
Na+/H+
Vesicles
acidification
aging
animal cell
animal experiment
animal tissue
article
blood pH
brush border
brush border vesicle
controlled study
disease course
kidney function
kidney proximal tubule
male
Michaelis constant
nonhuman
priority journal
proton transport
punch biopsy
rat
Acids
Aging
Animals
Bicarbonates
Blotting, Western
Hydrogen-Ion Concentration
Kidney Tubules, Proximal
Kinetics
Male
Microvilli
Osmolar Concentration
Punctures
Rats
Rats, Wistar
Sodium-Hydrogen Antiporter
Time Factors
description The normal aging process is accompanied by a progressive deterioration of renal function. We studied the kinetics of proximal tubular acidification of young (3 mo) and aging (22 mo) rats using in vivo and in vitro techniques. Blood acid-base parameters were similar in both groups. The maximum velocity of the Na+/H+ exchange (NHE) in brush-border membrane vesicles (BBMV) showed a 72% decrease in aging compared with young rats, whereas the Michaelis constant remained unchanged. The NHE3 isoform of the Na+/H+ exchanger was detected in BBMV by Western blot in both groups, and a decrease of 90% in the abundance was observed in aging rats. Micropuncture experiments with simultaneous luminal and peritubular perfusion with phosphate Ringer and continuous measurement of intratubular pH showed an acidification rate constant 34% smaller in aging compared with young rats. Proton flux was 48% lower in aging than in young rats. The present results suggest that proximal tubular acidification is impaired with aging.
format JOUR
author Mac Laughlin, M.
Damasco, M.C.
Igarreta, P.
Amorena, C.
author_facet Mac Laughlin, M.
Damasco, M.C.
Igarreta, P.
Amorena, C.
author_sort Mac Laughlin, M.
title In vitro and in vivo evaluation of proximal tubular acidification in aging rats
title_short In vitro and in vivo evaluation of proximal tubular acidification in aging rats
title_full In vitro and in vivo evaluation of proximal tubular acidification in aging rats
title_fullStr In vitro and in vivo evaluation of proximal tubular acidification in aging rats
title_full_unstemmed In vitro and in vivo evaluation of proximal tubular acidification in aging rats
title_sort in vitro and in vivo evaluation of proximal tubular acidification in aging rats
url http://hdl.handle.net/20.500.12110/paper_03636119_v280_n649-6_pR1627_MacLaughlin
work_keys_str_mv AT maclaughlinm invitroandinvivoevaluationofproximaltubularacidificationinagingrats
AT damascomc invitroandinvivoevaluationofproximaltubularacidificationinagingrats
AT igarretap invitroandinvivoevaluationofproximaltubularacidificationinagingrats
AT amorenac invitroandinvivoevaluationofproximaltubularacidificationinagingrats
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