Influence of inoculum preparation and volume on growth of mycotoxigenic molds

The influence of the volume and the a of the inoculum, adjusted or not with glycerol to the a w of the medium, on growth of mycotoxigenic species of fungi was determined for Aspergillus flavus, Aspergillus ochraceus, Penicillium citrinum, Penicillium viridicatum, Fusarium tricinctum and Microdochium...

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Autores principales: González, H.H.L., Pacin, A., Boente, G., Martínez, E., Resnik, S.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0362028X_v58_n4_p430_Gonzalez
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spelling todo:paper_0362028X_v58_n4_p430_Gonzalez2023-10-03T15:26:47Z Influence of inoculum preparation and volume on growth of mycotoxigenic molds González, H.H.L. Pacin, A. Boente, G. Martínez, E. Resnik, S. Inoculum volume Mycotoxigenic fungi Water activity Aspergillus flavus Aspergillus ochraceus Fungi Fusarium Fusarium tricinctum Microdochium nivale Penicillium Penicillium citrinum Penicillium viridicatum The influence of the volume and the a of the inoculum, adjusted or not with glycerol to the a w of the medium, on growth of mycotoxigenic species of fungi was determined for Aspergillus flavus, Aspergillus ochraceus, Penicillium citrinum, Penicillium viridicatum, Fusarium tricinctum and Microdochium nivale. Statistical analysis of the data was based on the application of multivariate techniques. It was seen that at constant volumes (10 μl), P. viridicatum, A. ochraceus and M. nivale show no significant growth differences whether or not the a w of the inoculum is adjusted; but significant differences were observed with A. flavus, P. citrinum and F. tricinctum. Moreover, significant differences in growth between adjusted and unadjusted a w levels are also present when there are different inoculum volumes for A. flavus and P. citrinum. Thus, it is appropriate to adjust the inoculum to the same a w level as that of the medium or the food to be considered. In all cases, where significant differences were present, greater colony diameters were observed when the a w was adjusted. With the same inoculum preparation, different inoculum volumes also affect fungus growth, producing greater colony diameters with increments in the volume. Copyright ©, International Association of Milk, Food and Environmental Sanitarians. Fil:González, H.H.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Boente, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martínez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Resnik, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0362028X_v58_n4_p430_Gonzalez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Inoculum volume
Mycotoxigenic fungi
Water activity
Aspergillus flavus
Aspergillus ochraceus
Fungi
Fusarium
Fusarium tricinctum
Microdochium nivale
Penicillium
Penicillium citrinum
Penicillium viridicatum
spellingShingle Inoculum volume
Mycotoxigenic fungi
Water activity
Aspergillus flavus
Aspergillus ochraceus
Fungi
Fusarium
Fusarium tricinctum
Microdochium nivale
Penicillium
Penicillium citrinum
Penicillium viridicatum
González, H.H.L.
Pacin, A.
Boente, G.
Martínez, E.
Resnik, S.
Influence of inoculum preparation and volume on growth of mycotoxigenic molds
topic_facet Inoculum volume
Mycotoxigenic fungi
Water activity
Aspergillus flavus
Aspergillus ochraceus
Fungi
Fusarium
Fusarium tricinctum
Microdochium nivale
Penicillium
Penicillium citrinum
Penicillium viridicatum
description The influence of the volume and the a of the inoculum, adjusted or not with glycerol to the a w of the medium, on growth of mycotoxigenic species of fungi was determined for Aspergillus flavus, Aspergillus ochraceus, Penicillium citrinum, Penicillium viridicatum, Fusarium tricinctum and Microdochium nivale. Statistical analysis of the data was based on the application of multivariate techniques. It was seen that at constant volumes (10 μl), P. viridicatum, A. ochraceus and M. nivale show no significant growth differences whether or not the a w of the inoculum is adjusted; but significant differences were observed with A. flavus, P. citrinum and F. tricinctum. Moreover, significant differences in growth between adjusted and unadjusted a w levels are also present when there are different inoculum volumes for A. flavus and P. citrinum. Thus, it is appropriate to adjust the inoculum to the same a w level as that of the medium or the food to be considered. In all cases, where significant differences were present, greater colony diameters were observed when the a w was adjusted. With the same inoculum preparation, different inoculum volumes also affect fungus growth, producing greater colony diameters with increments in the volume. Copyright ©, International Association of Milk, Food and Environmental Sanitarians.
format JOUR
author González, H.H.L.
Pacin, A.
Boente, G.
Martínez, E.
Resnik, S.
author_facet González, H.H.L.
Pacin, A.
Boente, G.
Martínez, E.
Resnik, S.
author_sort González, H.H.L.
title Influence of inoculum preparation and volume on growth of mycotoxigenic molds
title_short Influence of inoculum preparation and volume on growth of mycotoxigenic molds
title_full Influence of inoculum preparation and volume on growth of mycotoxigenic molds
title_fullStr Influence of inoculum preparation and volume on growth of mycotoxigenic molds
title_full_unstemmed Influence of inoculum preparation and volume on growth of mycotoxigenic molds
title_sort influence of inoculum preparation and volume on growth of mycotoxigenic molds
url http://hdl.handle.net/20.500.12110/paper_0362028X_v58_n4_p430_Gonzalez
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