Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis

Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis. Xanthomonas citri subsp. citri (Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes, f...

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Autores principales:	Petrocelli, S., Arana, M.R., Cabrini, M.N., Casabuono, A.C., Moyano, L., Beltramino, M., Moreira, L.M., Couto, A.S., Orellano, E.G.
Formato:	JOUR
Materias:	bacterial protein pilin protein Tfp unclassified drug fimbria protein Article bacterial colonization bacterial gene bacterial growth bacterial plant disease bacterial strain bacterial virulence bacterium adherence biofilm controlled study fimA gene fimA1 gene gene deletion gene identification gene mutation nonhuman pilA gene priority journal protein glycosylation protein purification structure analysis sweet orange Xanthomonas citri Xanthomonas citri subsp. citri Citrus genetics metabolism microbiology pathogenicity plant disease virulence Xanthomonas Bacterial Proteins Fimbriae Proteins Gene Deletion Plant Diseases Virulence
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03438651_v73_n6_p904_Petrocelli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03438651_v73_n6_p904_Petrocelli
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spelling	todo:paper_03438651_v73_n6_p904_Petrocelli2023-10-03T15:26:18Z Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis Petrocelli, S. Arana, M.R. Cabrini, M.N. Casabuono, A.C. Moyano, L. Beltramino, M. Moreira, L.M. Couto, A.S. Orellano, E.G. bacterial protein pilin protein Tfp unclassified drug fimbria protein Article bacterial colonization bacterial gene bacterial growth bacterial plant disease bacterial strain bacterial virulence bacterium adherence biofilm controlled study fimA gene fimA1 gene gene deletion gene identification gene mutation nonhuman pilA gene priority journal protein glycosylation protein purification structure analysis sweet orange Xanthomonas citri Xanthomonas citri subsp. citri Citrus gene deletion genetics metabolism microbiology pathogenicity plant disease virulence Xanthomonas Bacterial Proteins Citrus Fimbriae Proteins Gene Deletion Plant Diseases Virulence Xanthomonas Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis. Xanthomonas citri subsp. citri (Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes, fimA, fimA1, and pilA from Xcc were studied. A pilA mutant strain from Xcc (XccΔpilA) was constructed and differences in physiological features, such as motilities, adhesion, and biofilm formation, were observed. A structural study of the purified Tfp fractions from Xcc wild-type and Xcc∆pilA showed that pilins are glycosylated in both strains and that FimA and FimA1 are the main structural components of the pili. Furthermore, smaller lesion symptoms and reduced bacterial growth were produced by Xcc∆pilA in orange plants compared to the wild-type strain. These results indicate that the minor pilin-like gene, pilA, is involved in Tfp performance during the infection process. © 2016, Springer Science+Business Media New York. Fil:Casabuono, A.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03438651_v73_n6_p904_Petrocelli
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bacterial protein pilin protein Tfp unclassified drug fimbria protein Article bacterial colonization bacterial gene bacterial growth bacterial plant disease bacterial strain bacterial virulence bacterium adherence biofilm controlled study fimA gene fimA1 gene gene deletion gene identification gene mutation nonhuman pilA gene priority journal protein glycosylation protein purification structure analysis sweet orange Xanthomonas citri Xanthomonas citri subsp. citri Citrus gene deletion genetics metabolism microbiology pathogenicity plant disease virulence Xanthomonas Bacterial Proteins Citrus Fimbriae Proteins Gene Deletion Plant Diseases Virulence Xanthomonas
spellingShingle	bacterial protein pilin protein Tfp unclassified drug fimbria protein Article bacterial colonization bacterial gene bacterial growth bacterial plant disease bacterial strain bacterial virulence bacterium adherence biofilm controlled study fimA gene fimA1 gene gene deletion gene identification gene mutation nonhuman pilA gene priority journal protein glycosylation protein purification structure analysis sweet orange Xanthomonas citri Xanthomonas citri subsp. citri Citrus gene deletion genetics metabolism microbiology pathogenicity plant disease virulence Xanthomonas Bacterial Proteins Citrus Fimbriae Proteins Gene Deletion Plant Diseases Virulence Xanthomonas Petrocelli, S. Arana, M.R. Cabrini, M.N. Casabuono, A.C. Moyano, L. Beltramino, M. Moreira, L.M. Couto, A.S. Orellano, E.G. Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
topic_facet	bacterial protein pilin protein Tfp unclassified drug fimbria protein Article bacterial colonization bacterial gene bacterial growth bacterial plant disease bacterial strain bacterial virulence bacterium adherence biofilm controlled study fimA gene fimA1 gene gene deletion gene identification gene mutation nonhuman pilA gene priority journal protein glycosylation protein purification structure analysis sweet orange Xanthomonas citri Xanthomonas citri subsp. citri Citrus gene deletion genetics metabolism microbiology pathogenicity plant disease virulence Xanthomonas Bacterial Proteins Citrus Fimbriae Proteins Gene Deletion Plant Diseases Virulence Xanthomonas
description	Type IV pili (Tfp) are widely distributed adhesins of bacterial surfaces. In plant pathogenic bacteria, Tfp are involved in host colonization and pathogenesis. Xanthomonas citri subsp. citri (Xcc) is the phytopathogen responsible for citrus canker disease. In this work, three Tfp structural genes, fimA, fimA1, and pilA from Xcc were studied. A pilA mutant strain from Xcc (XccΔpilA) was constructed and differences in physiological features, such as motilities, adhesion, and biofilm formation, were observed. A structural study of the purified Tfp fractions from Xcc wild-type and Xcc∆pilA showed that pilins are glycosylated in both strains and that FimA and FimA1 are the main structural components of the pili. Furthermore, smaller lesion symptoms and reduced bacterial growth were produced by Xcc∆pilA in orange plants compared to the wild-type strain. These results indicate that the minor pilin-like gene, pilA, is involved in Tfp performance during the infection process. © 2016, Springer Science+Business Media New York.
format	JOUR
author	Petrocelli, S. Arana, M.R. Cabrini, M.N. Casabuono, A.C. Moyano, L. Beltramino, M. Moreira, L.M. Couto, A.S. Orellano, E.G.
author_facet	Petrocelli, S. Arana, M.R. Cabrini, M.N. Casabuono, A.C. Moyano, L. Beltramino, M. Moreira, L.M. Couto, A.S. Orellano, E.G.
author_sort	Petrocelli, S.
title	Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
title_short	Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
title_full	Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
title_fullStr	Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
title_full_unstemmed	Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis
title_sort	deletion of pila, a minor pilin-like gene, from xanthomonas citri subsp. citri influences bacterial physiology and pathogenesis
url	http://hdl.handle.net/20.500.12110/paper_03438651_v73_n6_p904_Petrocelli
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Deletion of pilA, a Minor Pilin-Like Gene, from Xanthomonas citri subsp. citri Influences Bacterial Physiology and Pathogenesis

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