SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus

Entomopathogenic bacilli B. thuringiensis israelensis (Bti) and B. sphaericus (Bf) exhibit low survival on field application. It was previously shown that their spores are very sensitive to different stress effectors (heat, UV light) and especially to osmotic variations. Since SASP (Small, Acid-Solu...

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Autores principales: Cucchi, A., De Rivas, C.S.
Formato: JOUR
Materias:
bacterial protein
amino acid sequence
article
bacillus sphaericus
bacillus thuringiensis
bacterial growth
environmental factor
nonhuman
osmoregulation
priority journal
protein determination
sporogenesis
stress
Bacillus
Bacillus thuringiensis
Bacterial Proteins
Osmotic Pressure
Peroxides
Sodium Chloride
Spores, Bacterial
Temperature
Ultraviolet Rays
Bacilli
Bacillus sphaericus
Bacillus subtilis
Bacillus thuringiensis serovar israelensis
Bacteria (microorganisms)
Posibacteria
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03438651_v36_n4_p220_Cucchi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03438651_v36_n4_p220_Cucchi
record_format dspace
spelling todo:paper_03438651_v36_n4_p220_Cucchi2023-10-03T15:26:14Z SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus Cucchi, A. De Rivas, C.S. bacterial protein amino acid sequence article bacillus sphaericus bacillus thuringiensis bacterial growth environmental factor nonhuman osmoregulation priority journal protein determination sporogenesis stress Bacillus Bacillus thuringiensis Bacterial Proteins Osmotic Pressure Peroxides Sodium Chloride Spores, Bacterial Temperature Ultraviolet Rays Bacilli Bacillus sphaericus Bacillus subtilis Bacillus thuringiensis Bacillus thuringiensis serovar israelensis Bacteria (microorganisms) Posibacteria Entomopathogenic bacilli B. thuringiensis israelensis (Bti) and B. sphaericus (Bf) exhibit low survival on field application. It was previously shown that their spores are very sensitive to different stress effectors (heat, UV light) and especially to osmotic variations. Since SASP (Small, Acid-Soluble Spore Proteins), α/β and γ type, are involved in spore tolerance to heat, UV light, peroxide, and salt, they were analyzed in Bti and Bf. The molecular weight, migration pattern and amino acid composition of different SASP were determined and compared with other bacilli, in particular to B. subtilis. A relation between spore osmotolerance, SASP content, and amino acid composition was shown. In addition, the absence of τ SASP in Bti and Bf is discussed. Fil:Cucchi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03438651_v36_n4_p220_Cucchi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic bacterial protein
amino acid sequence
article
bacillus sphaericus
bacillus thuringiensis
bacterial growth
environmental factor
nonhuman
osmoregulation
priority journal
protein determination
sporogenesis
stress
Bacillus
Bacillus thuringiensis
Bacterial Proteins
Osmotic Pressure
Peroxides
Sodium Chloride
Spores, Bacterial
Temperature
Ultraviolet Rays
Bacilli
Bacillus sphaericus
Bacillus subtilis
Bacillus thuringiensis
Bacillus thuringiensis serovar israelensis
Bacteria (microorganisms)
Posibacteria
spellingShingle bacterial protein
amino acid sequence
article
bacillus sphaericus
bacillus thuringiensis
bacterial growth
environmental factor
nonhuman
osmoregulation
priority journal
protein determination
sporogenesis
stress
Bacillus
Bacillus thuringiensis
Bacterial Proteins
Osmotic Pressure
Peroxides
Sodium Chloride
Spores, Bacterial
Temperature
Ultraviolet Rays
Bacilli
Bacillus sphaericus
Bacillus subtilis
Bacillus thuringiensis
Bacillus thuringiensis serovar israelensis
Bacteria (microorganisms)
Posibacteria
Cucchi, A.
De Rivas, C.S.
SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus
topic_facet bacterial protein
amino acid sequence
article
bacillus sphaericus
bacillus thuringiensis
bacterial growth
environmental factor
nonhuman
osmoregulation
priority journal
protein determination
sporogenesis
stress
Bacillus
Bacillus thuringiensis
Bacterial Proteins
Osmotic Pressure
Peroxides
Sodium Chloride
Spores, Bacterial
Temperature
Ultraviolet Rays
Bacilli
Bacillus sphaericus
Bacillus subtilis
Bacillus thuringiensis
Bacillus thuringiensis serovar israelensis
Bacteria (microorganisms)
Posibacteria
description Entomopathogenic bacilli B. thuringiensis israelensis (Bti) and B. sphaericus (Bf) exhibit low survival on field application. It was previously shown that their spores are very sensitive to different stress effectors (heat, UV light) and especially to osmotic variations. Since SASP (Small, Acid-Soluble Spore Proteins), α/β and γ type, are involved in spore tolerance to heat, UV light, peroxide, and salt, they were analyzed in Bti and Bf. The molecular weight, migration pattern and amino acid composition of different SASP were determined and compared with other bacilli, in particular to B. subtilis. A relation between spore osmotolerance, SASP content, and amino acid composition was shown. In addition, the absence of τ SASP in Bti and Bf is discussed.
format JOUR
author Cucchi, A.
De Rivas, C.S.
author_facet Cucchi, A.
De Rivas, C.S.
author_sort Cucchi, A.
title SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus
title_short SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus
title_full SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus
title_fullStr SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus
title_full_unstemmed SASP (small, acid-soluble spore proteins) and spore properties in Bacillus thuringiensis israelensis and Bacillus sphaericus
title_sort sasp (small, acid-soluble spore proteins) and spore properties in bacillus thuringiensis israelensis and bacillus sphaericus
url http://hdl.handle.net/20.500.12110/paper_03438651_v36_n4_p220_Cucchi
work_keys_str_mv AT cucchia saspsmallacidsolublesporeproteinsandsporepropertiesinbacillusthuringiensisisraelensisandbacillussphaericus
AT derivascs saspsmallacidsolublesporeproteinsandsporepropertiesinbacillusthuringiensisisraelensisandbacillussphaericus
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