Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis
In Bacillus subtilis, osmotolerance is a stationary phase-dependent, adaptive response inhibiting sporulation and sharing common regulators with this process. The extent of this inhibition was determined by measuring transcription activity of promoter lacZ fusions to early sigma genes (spoIIG and sp...
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todo:paper_03438651_v36_n2_p75_Ruzal2023-10-03T15:26:14Z Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis Ruzal, S.M. López, C. Rivas, E. Sánchez-Rivas, C. beta galactosidase sigma factor sodium chloride article bacillus subtilis controlled study electron microscopy gene expression genetic transcription hyperosmolarity nonhuman osmoregulation priority journal promoter region sporogenesis Bacillus subtilis Bacterial Proteins Gene Expression Gene Expression Regulation Genes, Bacterial Microscopy, Electron Osmolar Concentration Sigma Factor Sodium Chloride Spores Spores, Bacterial Time Factors Transcription Factors Bacillus subtilis Posibacteria Prokaryota In Bacillus subtilis, osmotolerance is a stationary phase-dependent, adaptive response inhibiting sporulation and sharing common regulators with this process. The extent of this inhibition was determined by measuring transcription activity of promoter lacZ fusions to early sigma genes (spoIIG and spoIIA coding for precursors of oσ(E) and σ(F)) and to reporters of them (spoIID, spoIIQ and spoIIIG), in the absence and presence of 0.6 M or 1 M NaCl. The transcription activity of these sigma precursors, normally occurring at the onset of the stationary phase, was reduced to 30-50% of their maximal expression in hyperosmotic conditions; expression of genes under their control was, however, more inhibited (< 10%). Therefore, sporulation was blocked at the sigma σ(E) and σ(F) activation steps. This assumption was confirmed by electron microscopic examinations of hyperosmotic cultures, which presented asymmetric septa characteristic of stage II mutants. Discussion was focused on the particular composition and/or structure of membranes during hyperosmotic growth and their involvement in the arrest of sporulation. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03438651_v36_n2_p75_Ruzal |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
beta galactosidase sigma factor sodium chloride article bacillus subtilis controlled study electron microscopy gene expression genetic transcription hyperosmolarity nonhuman osmoregulation priority journal promoter region sporogenesis Bacillus subtilis Bacterial Proteins Gene Expression Gene Expression Regulation Genes, Bacterial Microscopy, Electron Osmolar Concentration Sigma Factor Sodium Chloride Spores Spores, Bacterial Time Factors Transcription Factors Bacillus subtilis Posibacteria Prokaryota |
| spellingShingle |
beta galactosidase sigma factor sodium chloride article bacillus subtilis controlled study electron microscopy gene expression genetic transcription hyperosmolarity nonhuman osmoregulation priority journal promoter region sporogenesis Bacillus subtilis Bacterial Proteins Gene Expression Gene Expression Regulation Genes, Bacterial Microscopy, Electron Osmolar Concentration Sigma Factor Sodium Chloride Spores Spores, Bacterial Time Factors Transcription Factors Bacillus subtilis Posibacteria Prokaryota Ruzal, S.M. López, C. Rivas, E. Sánchez-Rivas, C. Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis |
| topic_facet |
beta galactosidase sigma factor sodium chloride article bacillus subtilis controlled study electron microscopy gene expression genetic transcription hyperosmolarity nonhuman osmoregulation priority journal promoter region sporogenesis Bacillus subtilis Bacterial Proteins Gene Expression Gene Expression Regulation Genes, Bacterial Microscopy, Electron Osmolar Concentration Sigma Factor Sodium Chloride Spores Spores, Bacterial Time Factors Transcription Factors Bacillus subtilis Posibacteria Prokaryota |
| description |
In Bacillus subtilis, osmotolerance is a stationary phase-dependent, adaptive response inhibiting sporulation and sharing common regulators with this process. The extent of this inhibition was determined by measuring transcription activity of promoter lacZ fusions to early sigma genes (spoIIG and spoIIA coding for precursors of oσ(E) and σ(F)) and to reporters of them (spoIID, spoIIQ and spoIIIG), in the absence and presence of 0.6 M or 1 M NaCl. The transcription activity of these sigma precursors, normally occurring at the onset of the stationary phase, was reduced to 30-50% of their maximal expression in hyperosmotic conditions; expression of genes under their control was, however, more inhibited (< 10%). Therefore, sporulation was blocked at the sigma σ(E) and σ(F) activation steps. This assumption was confirmed by electron microscopic examinations of hyperosmotic cultures, which presented asymmetric septa characteristic of stage II mutants. Discussion was focused on the particular composition and/or structure of membranes during hyperosmotic growth and their involvement in the arrest of sporulation. |
| format |
JOUR |
| author |
Ruzal, S.M. López, C. Rivas, E. Sánchez-Rivas, C. |
| author_facet |
Ruzal, S.M. López, C. Rivas, E. Sánchez-Rivas, C. |
| author_sort |
Ruzal, S.M. |
| title |
Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis |
| title_short |
Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis |
| title_full |
Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis |
| title_fullStr |
Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis |
| title_full_unstemmed |
Osmotic strength blocks sporulation at stage II by impeding activation of early sigma factors in Bacillus subtilis |
| title_sort |
osmotic strength blocks sporulation at stage ii by impeding activation of early sigma factors in bacillus subtilis |
| url |
http://hdl.handle.net/20.500.12110/paper_03438651_v36_n2_p75_Ruzal |
| work_keys_str_mv |
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1782029588926300160 |