ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus

It was shown previously that spores and vegetative cells of Bacillus sphaericus (Bf) and Bacillus thuringiensis israelensis (Bti) are very sensitive to osmotic variations. Since spore osmotolerance has been associated with their SASP (small acid soluble spore proteins) content coded by ssp genes, hy...

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Detalles Bibliográficos
Autores principales: Cucchi, A., De Rivas, C.S.
Formato: JOUR
Materias:
article
bacillus sphaericus
bacillus thuringiensis
bacterial gene
bacterial spore
hybridization
nonhuman
osmotic stress
priority journal
sporogenesis
ultraviolet radiation
Animal
Bacillus thuringiensis
Bacterial Proteins
Diptera
Genes, Bacterial
Larva
Pest Control, Biological
Species Specificity
Spores, Bacterial
Support, Non-U.S. Gov't
Transfection
Ultraviolet Rays
Water-Electrolyte Balance
Bacillus sphaericus
Bacillus subtilis
Bacillus thuringiensis serovar israelensis
Bacteria (microorganisms)
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03438651_v31_n4_p228_Cucchi
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03438651_v31_n4_p228_Cucchi
record_format dspace
spelling todo:paper_03438651_v31_n4_p228_Cucchi2023-10-03T15:26:13Z ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus Cucchi, A. De Rivas, C.S. article bacillus sphaericus bacillus thuringiensis bacterial gene bacterial spore hybridization nonhuman osmotic stress priority journal sporogenesis ultraviolet radiation Animal Bacillus thuringiensis Bacterial Proteins Diptera Genes, Bacterial Larva Pest Control, Biological Species Specificity Spores, Bacterial Support, Non-U.S. Gov't Transfection Ultraviolet Rays Water-Electrolyte Balance Bacillus sphaericus Bacillus subtilis Bacillus thuringiensis serovar israelensis Bacteria (microorganisms) Diptera It was shown previously that spores and vegetative cells of Bacillus sphaericus (Bf) and Bacillus thuringiensis israelensis (Bti) are very sensitive to osmotic variations. Since spore osmotolerance has been associated with their SASP (small acid soluble spore proteins) content coded by ssp genes, hybridization assays were performed with sspE and sspA genes from B. subtilis as probes and showed that Bti and Bf strains could lack an sspE-like gene. The B. subtilis sspE gene was then introduced into Bti 4Q2 strain; spores were obtained and showed a 65 to 650 times higher level of osmotolerance to NaCl, without affecting other important properties: hypoosmotic resistance in vegetative cells, spore UV resistance, and larvicidal activity against diptera larvae. © 1995 Springer-Verlag New York Inc. Fil:Cucchi, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03438651_v31_n4_p228_Cucchi
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic article
bacillus sphaericus
bacillus thuringiensis
bacterial gene
bacterial spore
hybridization
nonhuman
osmotic stress
priority journal
sporogenesis
ultraviolet radiation
Animal
Bacillus thuringiensis
Bacterial Proteins
Diptera
Genes, Bacterial
Larva
Pest Control, Biological
Species Specificity
Spores, Bacterial
Support, Non-U.S. Gov't
Transfection
Ultraviolet Rays
Water-Electrolyte Balance
Bacillus sphaericus
Bacillus subtilis
Bacillus thuringiensis serovar israelensis
Bacteria (microorganisms)
Diptera
spellingShingle article
bacillus sphaericus
bacillus thuringiensis
bacterial gene
bacterial spore
hybridization
nonhuman
osmotic stress
priority journal
sporogenesis
ultraviolet radiation
Animal
Bacillus thuringiensis
Bacterial Proteins
Diptera
Genes, Bacterial
Larva
Pest Control, Biological
Species Specificity
Spores, Bacterial
Support, Non-U.S. Gov't
Transfection
Ultraviolet Rays
Water-Electrolyte Balance
Bacillus sphaericus
Bacillus subtilis
Bacillus thuringiensis serovar israelensis
Bacteria (microorganisms)
Diptera
Cucchi, A.
De Rivas, C.S.
ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus
topic_facet article
bacillus sphaericus
bacillus thuringiensis
bacterial gene
bacterial spore
hybridization
nonhuman
osmotic stress
priority journal
sporogenesis
ultraviolet radiation
Animal
Bacillus thuringiensis
Bacterial Proteins
Diptera
Genes, Bacterial
Larva
Pest Control, Biological
Species Specificity
Spores, Bacterial
Support, Non-U.S. Gov't
Transfection
Ultraviolet Rays
Water-Electrolyte Balance
Bacillus sphaericus
Bacillus subtilis
Bacillus thuringiensis serovar israelensis
Bacteria (microorganisms)
Diptera
description It was shown previously that spores and vegetative cells of Bacillus sphaericus (Bf) and Bacillus thuringiensis israelensis (Bti) are very sensitive to osmotic variations. Since spore osmotolerance has been associated with their SASP (small acid soluble spore proteins) content coded by ssp genes, hybridization assays were performed with sspE and sspA genes from B. subtilis as probes and showed that Bti and Bf strains could lack an sspE-like gene. The B. subtilis sspE gene was then introduced into Bti 4Q2 strain; spores were obtained and showed a 65 to 650 times higher level of osmotolerance to NaCl, without affecting other important properties: hypoosmotic resistance in vegetative cells, spore UV resistance, and larvicidal activity against diptera larvae. © 1995 Springer-Verlag New York Inc.
format JOUR
author Cucchi, A.
De Rivas, C.S.
author_facet Cucchi, A.
De Rivas, C.S.
author_sort Cucchi, A.
title ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus
title_short ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus
title_full ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus
title_fullStr ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus
title_full_unstemmed ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus
title_sort ssp genes and spore osmotolerance in bacillus thuringiensis israelensis and bacillus sphaericus
url http://hdl.handle.net/20.500.12110/paper_03438651_v31_n4_p228_Cucchi
work_keys_str_mv AT cucchia sspgenesandsporeosmotoleranceinbacillusthuringiensisisraelensisandbacillussphaericus
AT derivascs sspgenesandsporeosmotoleranceinbacillusthuringiensisisraelensisandbacillussphaericus
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