Effect of nitroxyl on human platelets function

There is a growing body of evidence on the role of nitric oxide (NO) in human platelet physiology regulation. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The...

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Detalles Bibliográficos
Autores principales: Bermejo, E., Sáenz, D.A., Alberto, F., Rosenstein, R.E., Bari, S.E., Lazzari, M.A.
Formato: JOUR
Materias:
Nitric oxide
Nitroxyl
Platelet physiology
1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one
adenosine triphosphate
adrenalin
alpha2 integrin
beta1 integrin
CD36 antigen
CD63 antigen
cyclic GMP
cysteine
fibrinogen receptor
glycoprotein Ib
nitric oxide
nitroprusside sodium
nitroxyl
PADGEM protein
reagent
trioxodinitrate sodium
unclassified drug
article
comparative study
controlled study
hematological parameters
human
human cell
normal human
priority journal
protein expression
thrombocyte aggregation inhibition
thrombocyte function
Adenosine Triphosphate
Antigens, CD
Blood Platelets
Cyclic GMP
Cysteine
Dose-Response Relationship, Drug
Drug Interactions
Humans
Nitric Oxide
Nitric Oxide Donors
Nitrites
Nitrogen Oxides
Nitroprusside
P-Selectin
Platelet Aggregation
Platelet Membrane Glycoproteins
Time Factors
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03406245_v94_n3_p578_Bermejo
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:There is a growing body of evidence on the role of nitric oxide (NO) in human platelet physiology regulation. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The aim of the present study was to comparatively analyze the effect of HNO and NO on several functional parameters of human platelets. For this purpose, sodium trioxodinitrate (Angeli's salt, AS) and sodium nitroprusside (SNP) were used as HNO and NO releasers, respectively. Both AS and SNP significantly inhibited platelet aggregation and ATP release induced by different agonists and adrenaline. AS or SNP did not modify the expression of platelet glycoproteins (Ib, IIb-IIIa, Ia-IIa, IV), whereas they substantially decreased the levels of CD62P, CD63 and of PAC-I (a platelet activated glycoprotein IIb/IIIa epitope) after the stimulation with ADP. AS and SNP significantly increased cGMP accumulation in a IH-[1,2,4]oxadiazolo [4,3-a] quinoxalin-I-one (ODQ)-sensitive manner. However while L-cysteine reduced the effect of AS, it increased the effect of SNP on this parameter. Accordingly, a differential effect of L-cysteine was observed on the antiaggregatory effect of both compounds. In summary, these results indicate that HNO is an effective inhibitor of human platelet aggregation. © 2005 Schattauer GmbH, Stuttgart.

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