Effect of nitroxyl on human platelets function
There is a growing body of evidence on the role of nitric oxide (NO) in human platelet physiology regulation. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The...
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todo:paper_03406245_v94_n3_p578_Bermejo2023-10-03T15:25:47Z Effect of nitroxyl on human platelets function Bermejo, E. Sáenz, D.A. Alberto, F. Rosenstein, R.E. Bari, S.E. Lazzari, M.A. Nitric oxide Nitroxyl Platelet physiology 1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one adenosine triphosphate adrenalin alpha2 integrin beta1 integrin CD36 antigen CD63 antigen cyclic GMP cysteine fibrinogen receptor glycoprotein Ib nitric oxide nitroprusside sodium nitroxyl PADGEM protein reagent trioxodinitrate sodium unclassified drug article comparative study controlled study hematological parameters human human cell normal human priority journal protein expression thrombocyte aggregation inhibition thrombocyte function Adenosine Triphosphate Antigens, CD Blood Platelets Cyclic GMP Cysteine Dose-Response Relationship, Drug Drug Interactions Humans Nitric Oxide Nitric Oxide Donors Nitrites Nitrogen Oxides Nitroprusside P-Selectin Platelet Aggregation Platelet Membrane Glycoproteins Time Factors There is a growing body of evidence on the role of nitric oxide (NO) in human platelet physiology regulation. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The aim of the present study was to comparatively analyze the effect of HNO and NO on several functional parameters of human platelets. For this purpose, sodium trioxodinitrate (Angeli's salt, AS) and sodium nitroprusside (SNP) were used as HNO and NO releasers, respectively. Both AS and SNP significantly inhibited platelet aggregation and ATP release induced by different agonists and adrenaline. AS or SNP did not modify the expression of platelet glycoproteins (Ib, IIb-IIIa, Ia-IIa, IV), whereas they substantially decreased the levels of CD62P, CD63 and of PAC-I (a platelet activated glycoprotein IIb/IIIa epitope) after the stimulation with ADP. AS and SNP significantly increased cGMP accumulation in a IH-[1,2,4]oxadiazolo [4,3-a] quinoxalin-I-one (ODQ)-sensitive manner. However while L-cysteine reduced the effect of AS, it increased the effect of SNP on this parameter. Accordingly, a differential effect of L-cysteine was observed on the antiaggregatory effect of both compounds. In summary, these results indicate that HNO is an effective inhibitor of human platelet aggregation. © 2005 Schattauer GmbH, Stuttgart. Fil:Sáenz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rosenstein, R.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bari, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03406245_v94_n3_p578_Bermejo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Nitric oxide Nitroxyl Platelet physiology 1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one adenosine triphosphate adrenalin alpha2 integrin beta1 integrin CD36 antigen CD63 antigen cyclic GMP cysteine fibrinogen receptor glycoprotein Ib nitric oxide nitroprusside sodium nitroxyl PADGEM protein reagent trioxodinitrate sodium unclassified drug article comparative study controlled study hematological parameters human human cell normal human priority journal protein expression thrombocyte aggregation inhibition thrombocyte function Adenosine Triphosphate Antigens, CD Blood Platelets Cyclic GMP Cysteine Dose-Response Relationship, Drug Drug Interactions Humans Nitric Oxide Nitric Oxide Donors Nitrites Nitrogen Oxides Nitroprusside P-Selectin Platelet Aggregation Platelet Membrane Glycoproteins Time Factors |
| spellingShingle |
Nitric oxide Nitroxyl Platelet physiology 1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one adenosine triphosphate adrenalin alpha2 integrin beta1 integrin CD36 antigen CD63 antigen cyclic GMP cysteine fibrinogen receptor glycoprotein Ib nitric oxide nitroprusside sodium nitroxyl PADGEM protein reagent trioxodinitrate sodium unclassified drug article comparative study controlled study hematological parameters human human cell normal human priority journal protein expression thrombocyte aggregation inhibition thrombocyte function Adenosine Triphosphate Antigens, CD Blood Platelets Cyclic GMP Cysteine Dose-Response Relationship, Drug Drug Interactions Humans Nitric Oxide Nitric Oxide Donors Nitrites Nitrogen Oxides Nitroprusside P-Selectin Platelet Aggregation Platelet Membrane Glycoproteins Time Factors Bermejo, E. Sáenz, D.A. Alberto, F. Rosenstein, R.E. Bari, S.E. Lazzari, M.A. Effect of nitroxyl on human platelets function |
| topic_facet |
Nitric oxide Nitroxyl Platelet physiology 1h 1,2,4 oxadiazolo[4,3 a]quinoxalin 1 one adenosine triphosphate adrenalin alpha2 integrin beta1 integrin CD36 antigen CD63 antigen cyclic GMP cysteine fibrinogen receptor glycoprotein Ib nitric oxide nitroprusside sodium nitroxyl PADGEM protein reagent trioxodinitrate sodium unclassified drug article comparative study controlled study hematological parameters human human cell normal human priority journal protein expression thrombocyte aggregation inhibition thrombocyte function Adenosine Triphosphate Antigens, CD Blood Platelets Cyclic GMP Cysteine Dose-Response Relationship, Drug Drug Interactions Humans Nitric Oxide Nitric Oxide Donors Nitrites Nitrogen Oxides Nitroprusside P-Selectin Platelet Aggregation Platelet Membrane Glycoproteins Time Factors |
| description |
There is a growing body of evidence on the role of nitric oxide (NO) in human platelet physiology regulation. Recently, interest has developed in the functional role of an alternative redox form of NO, namely nitroxyl (HNO/NO-), because it is formed by a number of diverse biochemical reactions. The aim of the present study was to comparatively analyze the effect of HNO and NO on several functional parameters of human platelets. For this purpose, sodium trioxodinitrate (Angeli's salt, AS) and sodium nitroprusside (SNP) were used as HNO and NO releasers, respectively. Both AS and SNP significantly inhibited platelet aggregation and ATP release induced by different agonists and adrenaline. AS or SNP did not modify the expression of platelet glycoproteins (Ib, IIb-IIIa, Ia-IIa, IV), whereas they substantially decreased the levels of CD62P, CD63 and of PAC-I (a platelet activated glycoprotein IIb/IIIa epitope) after the stimulation with ADP. AS and SNP significantly increased cGMP accumulation in a IH-[1,2,4]oxadiazolo [4,3-a] quinoxalin-I-one (ODQ)-sensitive manner. However while L-cysteine reduced the effect of AS, it increased the effect of SNP on this parameter. Accordingly, a differential effect of L-cysteine was observed on the antiaggregatory effect of both compounds. In summary, these results indicate that HNO is an effective inhibitor of human platelet aggregation. © 2005 Schattauer GmbH, Stuttgart. |
| format |
JOUR |
| author |
Bermejo, E. Sáenz, D.A. Alberto, F. Rosenstein, R.E. Bari, S.E. Lazzari, M.A. |
| author_facet |
Bermejo, E. Sáenz, D.A. Alberto, F. Rosenstein, R.E. Bari, S.E. Lazzari, M.A. |
| author_sort |
Bermejo, E. |
| title |
Effect of nitroxyl on human platelets function |
| title_short |
Effect of nitroxyl on human platelets function |
| title_full |
Effect of nitroxyl on human platelets function |
| title_fullStr |
Effect of nitroxyl on human platelets function |
| title_full_unstemmed |
Effect of nitroxyl on human platelets function |
| title_sort |
effect of nitroxyl on human platelets function |
| url |
http://hdl.handle.net/20.500.12110/paper_03406245_v94_n3_p578_Bermejo |
| work_keys_str_mv |
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1782028573345841152 |