Low anticoagulant activity of high sulphated heparan sulphates

Two high sulphated heparin-like polysaccharides (L1, MW 16,000 and L2, MW 11,700) were isolated from rat liver tissues, after DEAE-cellulose chromatography. Heparan sulphates from heart and lung tissues were isolated for comparison and fractionated according to their molecular weight. The anticoagul...

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Autores principales:	Kovensky, J., Sassetti, B., Fernandez Cirelli, A., Kordich, L.
Formato:	JOUR
Materias:	anticoagulant agent heparan sulfate heparin proteoglycan article gel chromatography human priority journal rat Animal Anticoagulants Glycosaminoglycans Heparan Sulfate Proteoglycan Heparitin Sulfate Liver Proteochondroitin Sulfates Rats Support, Non-U.S. Gov't
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03406245_v63_n3_p488_Kovensky
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03406245_v63_n3_p488_Kovensky
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spelling	todo:paper_03406245_v63_n3_p488_Kovensky2023-10-03T15:25:46Z Low anticoagulant activity of high sulphated heparan sulphates Kovensky, J. Sassetti, B. Fernandez Cirelli, A. Kordich, L. anticoagulant agent heparan sulfate heparin proteoglycan article gel chromatography human priority journal rat Animal Anticoagulants Glycosaminoglycans Heparan Sulfate Proteoglycan Heparitin Sulfate Liver Proteochondroitin Sulfates Rats Support, Non-U.S. Gov't Two high sulphated heparin-like polysaccharides (L1, MW 16,000 and L2, MW 11,700) were isolated from rat liver tissues, after DEAE-cellulose chromatography. Heparan sulphates from heart and lung tissues were isolated for comparison and fractionated according to their molecular weight. The anticoagulant activities in vitro were studied using clotting antifactor Xa, antifactor IIa, and APTT assay methods, falling in a narrow range (5-44 IU/mg) although the wide variability in molecular weight and sulphate content. The heparan sulphate nature of fractions L1 and L2 (sulphate/disaccharide ratio 2.05 and 2.48, respectively) has been verified by: a) low iduronic/glucuronic acid ratio; b) nitrous acid degradataion followed by gel chromatography; c) heparinase treatment followed by gel chromatography; d) electrophoretic behaviour. Native proteoglycans have been isolated and the glycosidic chains compared with L1 and L2. Their anticoagulant activities in vitro and the fact that antiXa clotting activity was not neutralized by protamine sulphate are in accordance with the results of structural studies. Fil:Kovensky, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sassetti, B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Fernandez Cirelli, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Kordich, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03406245_v63_n3_p488_Kovensky
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	anticoagulant agent heparan sulfate heparin proteoglycan article gel chromatography human priority journal rat Animal Anticoagulants Glycosaminoglycans Heparan Sulfate Proteoglycan Heparitin Sulfate Liver Proteochondroitin Sulfates Rats Support, Non-U.S. Gov't
spellingShingle	anticoagulant agent heparan sulfate heparin proteoglycan article gel chromatography human priority journal rat Animal Anticoagulants Glycosaminoglycans Heparan Sulfate Proteoglycan Heparitin Sulfate Liver Proteochondroitin Sulfates Rats Support, Non-U.S. Gov't Kovensky, J. Sassetti, B. Fernandez Cirelli, A. Kordich, L. Low anticoagulant activity of high sulphated heparan sulphates
topic_facet	anticoagulant agent heparan sulfate heparin proteoglycan article gel chromatography human priority journal rat Animal Anticoagulants Glycosaminoglycans Heparan Sulfate Proteoglycan Heparitin Sulfate Liver Proteochondroitin Sulfates Rats Support, Non-U.S. Gov't
description	Two high sulphated heparin-like polysaccharides (L1, MW 16,000 and L2, MW 11,700) were isolated from rat liver tissues, after DEAE-cellulose chromatography. Heparan sulphates from heart and lung tissues were isolated for comparison and fractionated according to their molecular weight. The anticoagulant activities in vitro were studied using clotting antifactor Xa, antifactor IIa, and APTT assay methods, falling in a narrow range (5-44 IU/mg) although the wide variability in molecular weight and sulphate content. The heparan sulphate nature of fractions L1 and L2 (sulphate/disaccharide ratio 2.05 and 2.48, respectively) has been verified by: a) low iduronic/glucuronic acid ratio; b) nitrous acid degradataion followed by gel chromatography; c) heparinase treatment followed by gel chromatography; d) electrophoretic behaviour. Native proteoglycans have been isolated and the glycosidic chains compared with L1 and L2. Their anticoagulant activities in vitro and the fact that antiXa clotting activity was not neutralized by protamine sulphate are in accordance with the results of structural studies.
format	JOUR
author	Kovensky, J. Sassetti, B. Fernandez Cirelli, A. Kordich, L.
author_facet	Kovensky, J. Sassetti, B. Fernandez Cirelli, A. Kordich, L.
author_sort	Kovensky, J.
title	Low anticoagulant activity of high sulphated heparan sulphates
title_short	Low anticoagulant activity of high sulphated heparan sulphates
title_full	Low anticoagulant activity of high sulphated heparan sulphates
title_fullStr	Low anticoagulant activity of high sulphated heparan sulphates
title_full_unstemmed	Low anticoagulant activity of high sulphated heparan sulphates
title_sort	low anticoagulant activity of high sulphated heparan sulphates
url	http://hdl.handle.net/20.500.12110/paper_03406245_v63_n3_p488_Kovensky
work_keys_str_mv	AT kovenskyj lowanticoagulantactivityofhighsulphatedheparansulphates AT sassettib lowanticoagulantactivityofhighsulphatedheparansulphates AT fernandezcirellia lowanticoagulantactivityofhighsulphatedheparansulphates AT kordichl lowanticoagulantactivityofhighsulphatedheparansulphates
_version_	1782029491022856192

Low anticoagulant activity of high sulphated heparan sulphates

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