Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation
Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of...
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todo:paper_03279545_v31_n2_p237_Julianelli2023-10-03T15:25:15Z Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation Julianelli, V.L. Guerra, L.N. Calvo, J.C. Differentiation Mammary epithelium Triglycerides glycerol 3 phosphate dehydrogenase triacylglycerol animal cell article breast epithelium cell communication cell proliferation cell strain 3T3 cell type controlled study enzyme activity human human cell mouse nonhuman proadipocyte 3T3 Cells 3T3-L1 Cells Adipocytes, White Animals Cell Communication Cell Differentiation Cell Proliferation Cells, Cultured Culture Media, Conditioned Epithelial Cells Hela Cells Humans Mammary Glands, Animal Mice Triglycerides Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation when NMMG cells are cultured in the presence of conditioned media from Swiss 3T3 or 3T3-L1 cells. We found a possible point of regulation for the mammary factor on a key enzyme of the lipid metabolic pathway, the glycerol-3-phosphate dehydrogenase. The inhibitory factor seems to have an effect on this enzyme's activity and reduces it. The results presented herein contribute to the understanding of cell-cell communication in a model of a normal mammary gland. Fil:Julianelli, V.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Guerra, L.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03279545_v31_n2_p237_Julianelli |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Differentiation Mammary epithelium Triglycerides glycerol 3 phosphate dehydrogenase triacylglycerol animal cell article breast epithelium cell communication cell proliferation cell strain 3T3 cell type controlled study enzyme activity human human cell mouse nonhuman proadipocyte 3T3 Cells 3T3-L1 Cells Adipocytes, White Animals Cell Communication Cell Differentiation Cell Proliferation Cells, Cultured Culture Media, Conditioned Epithelial Cells Hela Cells Humans Mammary Glands, Animal Mice Triglycerides |
| spellingShingle |
Differentiation Mammary epithelium Triglycerides glycerol 3 phosphate dehydrogenase triacylglycerol animal cell article breast epithelium cell communication cell proliferation cell strain 3T3 cell type controlled study enzyme activity human human cell mouse nonhuman proadipocyte 3T3 Cells 3T3-L1 Cells Adipocytes, White Animals Cell Communication Cell Differentiation Cell Proliferation Cells, Cultured Culture Media, Conditioned Epithelial Cells Hela Cells Humans Mammary Glands, Animal Mice Triglycerides Julianelli, V.L. Guerra, L.N. Calvo, J.C. Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation |
| topic_facet |
Differentiation Mammary epithelium Triglycerides glycerol 3 phosphate dehydrogenase triacylglycerol animal cell article breast epithelium cell communication cell proliferation cell strain 3T3 cell type controlled study enzyme activity human human cell mouse nonhuman proadipocyte 3T3 Cells 3T3-L1 Cells Adipocytes, White Animals Cell Communication Cell Differentiation Cell Proliferation Cells, Cultured Culture Media, Conditioned Epithelial Cells Hela Cells Humans Mammary Glands, Animal Mice Triglycerides |
| description |
Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation when NMMG cells are cultured in the presence of conditioned media from Swiss 3T3 or 3T3-L1 cells. We found a possible point of regulation for the mammary factor on a key enzyme of the lipid metabolic pathway, the glycerol-3-phosphate dehydrogenase. The inhibitory factor seems to have an effect on this enzyme's activity and reduces it. The results presented herein contribute to the understanding of cell-cell communication in a model of a normal mammary gland. |
| format |
JOUR |
| author |
Julianelli, V.L. Guerra, L.N. Calvo, J.C. |
| author_facet |
Julianelli, V.L. Guerra, L.N. Calvo, J.C. |
| author_sort |
Julianelli, V.L. |
| title |
Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation |
| title_short |
Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation |
| title_full |
Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation |
| title_fullStr |
Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation |
| title_full_unstemmed |
Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: Effect on triglyceride accumulation and cell proliferation |
| title_sort |
cell-cell communication between mouse mammary epithelial cells and 3t3-l1 preadipocytes: effect on triglyceride accumulation and cell proliferation |
| url |
http://hdl.handle.net/20.500.12110/paper_03279545_v31_n2_p237_Julianelli |
| work_keys_str_mv |
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