Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase

The localization and subcellular distribution of Trypanosoma cruzi nitric oxide synthase was investigated in epimastigote cells by immunocytochemistry at electron and light microscope level, using a polyclonal antibody to neuronal nitric oxide synthase, and also, at light microscope level, by the ni...

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Autores principales:	Goldstein, J., Paveto, C., López-Costa, J.J., Pereira, C., Alonso, G., Torres, H.N., Flawia, M.M.
Formato:	JOUR
Materias:	Diaphorase Immunocytochemistry Nitric oxide synthase Subcellular localization Trypanosoma cruzi formazan nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal cell apical membrane article cell membrane cell motility cell ultrastructure cellular distribution enzyme localization epimastigote flagellum immunocytochemistry nonhuman parasite migration signal transduction Animals Cell Compartmentation Cell Membrane Cell Movement Flagella Nitric Oxide Nitric Oxide Synthase Signal Transduction Animalia Trypanosoma
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03279545_v24_n3_p217_Goldstein
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03279545_v24_n3_p217_Goldstein
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spelling	todo:paper_03279545_v24_n3_p217_Goldstein2023-10-03T15:25:11Z Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase Goldstein, J. Paveto, C. López-Costa, J.J. Pereira, C. Alonso, G. Torres, H.N. Flawia, M.M. Diaphorase Immunocytochemistry Nitric oxide synthase Subcellular localization Trypanosoma cruzi formazan nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal cell apical membrane article cell membrane cell motility cell ultrastructure cellular distribution enzyme localization epimastigote flagellum immunocytochemistry nonhuman parasite migration signal transduction Trypanosoma cruzi Animals Cell Compartmentation Cell Membrane Cell Movement Flagella Nitric Oxide Nitric Oxide Synthase Signal Transduction Trypanosoma cruzi Animalia Trypanosoma Trypanosoma cruzi The localization and subcellular distribution of Trypanosoma cruzi nitric oxide synthase was investigated in epimastigote cells by immunocytochemistry at electron and light microscope level, using a polyclonal antibody to neuronal nitric oxide synthase, and also, at light microscope level, by the nicotinamide adenine dinucleotide phosphate-diaphorase histochemical reaction. The immunoreactivity was ultrastructurally localized by electron microscopy in the inner surface of cell membranes and in free cytosolic clusters in the body, flagellum and apical extreme. Light microscopy showed that immunoprecipitates, specific for the Trypanosoma cruzi nitric oxide synthase, co-localized with the formazan precipitates generated by the diaphorase reaction in the same areas identified by electron microscopy. These results, taken together with previous finding from our laboratory could help to explain the involvement of the nitric oxide transduction pathway in T. cruzi epimastigote motility. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03279545_v24_n3_p217_Goldstein
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Diaphorase Immunocytochemistry Nitric oxide synthase Subcellular localization Trypanosoma cruzi formazan nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal cell apical membrane article cell membrane cell motility cell ultrastructure cellular distribution enzyme localization epimastigote flagellum immunocytochemistry nonhuman parasite migration signal transduction Trypanosoma cruzi Animals Cell Compartmentation Cell Membrane Cell Movement Flagella Nitric Oxide Nitric Oxide Synthase Signal Transduction Trypanosoma cruzi Animalia Trypanosoma Trypanosoma cruzi
spellingShingle	Diaphorase Immunocytochemistry Nitric oxide synthase Subcellular localization Trypanosoma cruzi formazan nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal cell apical membrane article cell membrane cell motility cell ultrastructure cellular distribution enzyme localization epimastigote flagellum immunocytochemistry nonhuman parasite migration signal transduction Trypanosoma cruzi Animals Cell Compartmentation Cell Membrane Cell Movement Flagella Nitric Oxide Nitric Oxide Synthase Signal Transduction Trypanosoma cruzi Animalia Trypanosoma Trypanosoma cruzi Goldstein, J. Paveto, C. López-Costa, J.J. Pereira, C. Alonso, G. Torres, H.N. Flawia, M.M. Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase
topic_facet	Diaphorase Immunocytochemistry Nitric oxide synthase Subcellular localization Trypanosoma cruzi formazan nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase animal cell apical membrane article cell membrane cell motility cell ultrastructure cellular distribution enzyme localization epimastigote flagellum immunocytochemistry nonhuman parasite migration signal transduction Trypanosoma cruzi Animals Cell Compartmentation Cell Membrane Cell Movement Flagella Nitric Oxide Nitric Oxide Synthase Signal Transduction Trypanosoma cruzi Animalia Trypanosoma Trypanosoma cruzi
description	The localization and subcellular distribution of Trypanosoma cruzi nitric oxide synthase was investigated in epimastigote cells by immunocytochemistry at electron and light microscope level, using a polyclonal antibody to neuronal nitric oxide synthase, and also, at light microscope level, by the nicotinamide adenine dinucleotide phosphate-diaphorase histochemical reaction. The immunoreactivity was ultrastructurally localized by electron microscopy in the inner surface of cell membranes and in free cytosolic clusters in the body, flagellum and apical extreme. Light microscopy showed that immunoprecipitates, specific for the Trypanosoma cruzi nitric oxide synthase, co-localized with the formazan precipitates generated by the diaphorase reaction in the same areas identified by electron microscopy. These results, taken together with previous finding from our laboratory could help to explain the involvement of the nitric oxide transduction pathway in T. cruzi epimastigote motility.
format	JOUR
author	Goldstein, J. Paveto, C. López-Costa, J.J. Pereira, C. Alonso, G. Torres, H.N. Flawia, M.M.
author_facet	Goldstein, J. Paveto, C. López-Costa, J.J. Pereira, C. Alonso, G. Torres, H.N. Flawia, M.M.
author_sort	Goldstein, J.
title	Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase
title_short	Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase
title_full	Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase
title_fullStr	Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase
title_full_unstemmed	Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase
title_sort	immuno and cytochemical localization of trypanosoma cruzi nitric oxide synthase
url	http://hdl.handle.net/20.500.12110/paper_03279545_v24_n3_p217_Goldstein
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_version_	1782023783434944512

Immuno and cytochemical localization of Trypanosoma cruzi nitric oxide synthase

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