Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes
Glial cell-derived nitric oxide (NO·) and its regulation has significant implications for central nervous system pathophysiology. The aim of the present study was to determine the production of NO· by cultured rat astrocytes and peritoneal macrophages, and its regulation by glucocorticoids. Results...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03279545_v23_n1_p29_Llanos |
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todo:paper_03279545_v23_n1_p29_Llanos2023-10-03T15:25:10Z Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes Llanos, S.L. Roldán, A. Astrocytes Dexamethasone Macrophages NADPH-diaphorase NO· Phagocytosis dexamethasone glucocorticoid isoenzyme nitric oxide nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase zymosan animal cell article astrocyte controlled study enzyme activation enzyme activity hormonal regulation newborn nonhuman peritoneum macrophage phagocytosis protein expression rat Animals Animals, Newborn Astrocytes Cells, Cultured Cerebral Cortex Dexamethasone Glucocorticoids Nitric Oxide Rats Rats, Sprague-Dawley Glial cell-derived nitric oxide (NO·) and its regulation has significant implications for central nervous system pathophysiology. The aim of the present study was to determine the production of NO· by cultured rat astrocytes and peritoneal macrophages, and its regulation by glucocorticoids. Results corroborate the presence of both constitutive and induced NO· synthase (cNOS and iNOS) in astrocytes, while macrophages express only the inducible isoform. Dexamethasone was able to decrease NO· synthesis produced by induction of iNOS in macrophage and astrocyte phagocytosis, but not the NO· produced by the cNOS spontaneously expressed in cultured astrocytes. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03279545_v23_n1_p29_Llanos |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Astrocytes Dexamethasone Macrophages NADPH-diaphorase NO· Phagocytosis dexamethasone glucocorticoid isoenzyme nitric oxide nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase zymosan animal cell article astrocyte controlled study enzyme activation enzyme activity hormonal regulation newborn nonhuman peritoneum macrophage phagocytosis protein expression rat Animals Animals, Newborn Astrocytes Cells, Cultured Cerebral Cortex Dexamethasone Glucocorticoids Nitric Oxide Rats Rats, Sprague-Dawley |
| spellingShingle |
Astrocytes Dexamethasone Macrophages NADPH-diaphorase NO· Phagocytosis dexamethasone glucocorticoid isoenzyme nitric oxide nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase zymosan animal cell article astrocyte controlled study enzyme activation enzyme activity hormonal regulation newborn nonhuman peritoneum macrophage phagocytosis protein expression rat Animals Animals, Newborn Astrocytes Cells, Cultured Cerebral Cortex Dexamethasone Glucocorticoids Nitric Oxide Rats Rats, Sprague-Dawley Llanos, S.L. Roldán, A. Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes |
| topic_facet |
Astrocytes Dexamethasone Macrophages NADPH-diaphorase NO· Phagocytosis dexamethasone glucocorticoid isoenzyme nitric oxide nitric oxide synthase reduced nicotinamide adenine dinucleotide phosphate dehydrogenase zymosan animal cell article astrocyte controlled study enzyme activation enzyme activity hormonal regulation newborn nonhuman peritoneum macrophage phagocytosis protein expression rat Animals Animals, Newborn Astrocytes Cells, Cultured Cerebral Cortex Dexamethasone Glucocorticoids Nitric Oxide Rats Rats, Sprague-Dawley |
| description |
Glial cell-derived nitric oxide (NO·) and its regulation has significant implications for central nervous system pathophysiology. The aim of the present study was to determine the production of NO· by cultured rat astrocytes and peritoneal macrophages, and its regulation by glucocorticoids. Results corroborate the presence of both constitutive and induced NO· synthase (cNOS and iNOS) in astrocytes, while macrophages express only the inducible isoform. Dexamethasone was able to decrease NO· synthesis produced by induction of iNOS in macrophage and astrocyte phagocytosis, but not the NO· produced by the cNOS spontaneously expressed in cultured astrocytes. |
| format |
JOUR |
| author |
Llanos, S.L. Roldán, A. |
| author_facet |
Llanos, S.L. Roldán, A. |
| author_sort |
Llanos, S.L. |
| title |
Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes |
| title_short |
Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes |
| title_full |
Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes |
| title_fullStr |
Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes |
| title_full_unstemmed |
Effect of dexamethasone on nitric oxide (NO·) Production by cultured astrocytes |
| title_sort |
effect of dexamethasone on nitric oxide (no·) production by cultured astrocytes |
| url |
http://hdl.handle.net/20.500.12110/paper_03279545_v23_n1_p29_Llanos |
| work_keys_str_mv |
AT llanossl effectofdexamethasoneonnitricoxidenoproductionbyculturedastrocytes AT roldana effectofdexamethasoneonnitricoxidenoproductionbyculturedastrocytes |
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1782030088734244864 |