Action of blood serum from rats with turpentine sterile inflammation on the development of CFU erythrocyte colonies. Possible role of erythropoietin.

Bone marrow CFUe mice cultures were prepared in Petri dishes and the number of full developed erythrocyte colonies were counted under various experimental conditions. In certain experiments, erythropoietin (EP = 0.4 U x ml-1 in the suspending medium) or sera from normal rats (100 microliters) or fro...

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Detalles Bibliográficos
Autores principales: Gutnisky, A., Gimeno, A.L.
Formato: JOUR
Materias:
erythropoietin
turpentine
abscess
animal
article
blood
bone marrow cell
erythroid precursor cell
female
rat
rat strain
Abscess
Animal
Blood
Bone Marrow Cells
Erythroid Progenitor Cells
Erythropoietin
Female
Rats
Rats, Inbred Strains
Turpentine
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03266656_v39_n2_p127_Gutnisky
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03266656_v39_n2_p127_Gutnisky
record_format dspace
spelling todo:paper_03266656_v39_n2_p127_Gutnisky2023-10-03T15:24:33Z Action of blood serum from rats with turpentine sterile inflammation on the development of CFU erythrocyte colonies. Possible role of erythropoietin. Gutnisky, A. Gimeno, A.L. erythropoietin turpentine abscess animal article blood bone marrow cell erythroid precursor cell female rat rat strain Abscess Animal Blood Bone Marrow Cells Erythroid Progenitor Cells Erythropoietin Female Rats Rats, Inbred Strains Turpentine Bone marrow CFUe mice cultures were prepared in Petri dishes and the number of full developed erythrocyte colonies were counted under various experimental conditions. In certain experiments, erythropoietin (EP = 0.4 U x ml-1 in the suspending medium) or sera from normal rats (100 microliters) or from animals with chronic turpentine sterile abscesses (60 or 100 microliters), were delivered to the CFUe colonies. The number of colonies in the group without EP was almost 5 times smaller than in the group with EP. Inasmuch as some few colonies are still able to develop, even in absence of added EP, the suggestion is advanced holding that the phenomenon may represent the effect of EP already bound to the group of cells initiating differentiation. Comparisons among all experimental groups indicate that the delivery of sera from turpentine rats to cultures containing added EP, reduced significantly the number of CFUe colonies seen in controls with EP but without inflammatory serum. It is suggested that the present findings could be explained assuming an inhibition of the influence of exogenous EP, subserved by the inflammatory serum, or alternatively that this kind of rat serum induces a partial blockade of EP at receptor sites whose activation is a mandatory step for the adequate and full development of cultured erythrocytes. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03266656_v39_n2_p127_Gutnisky
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic erythropoietin
turpentine
abscess
animal
article
blood
bone marrow cell
erythroid precursor cell
female
rat
rat strain
Abscess
Animal
Blood
Bone Marrow Cells
Erythroid Progenitor Cells
Erythropoietin
Female
Rats
Rats, Inbred Strains
Turpentine
spellingShingle erythropoietin
turpentine
abscess
animal
article
blood
bone marrow cell
erythroid precursor cell
female
rat
rat strain
Abscess
Animal
Blood
Bone Marrow Cells
Erythroid Progenitor Cells
Erythropoietin
Female
Rats
Rats, Inbred Strains
Turpentine
Gutnisky, A.
Gimeno, A.L.
Action of blood serum from rats with turpentine sterile inflammation on the development of CFU erythrocyte colonies. Possible role of erythropoietin.
topic_facet erythropoietin
turpentine
abscess
animal
article
blood
bone marrow cell
erythroid precursor cell
female
rat
rat strain
Abscess
Animal
Blood
Bone Marrow Cells
Erythroid Progenitor Cells
Erythropoietin
Female
Rats
Rats, Inbred Strains
Turpentine
description Bone marrow CFUe mice cultures were prepared in Petri dishes and the number of full developed erythrocyte colonies were counted under various experimental conditions. In certain experiments, erythropoietin (EP = 0.4 U x ml-1 in the suspending medium) or sera from normal rats (100 microliters) or from animals with chronic turpentine sterile abscesses (60 or 100 microliters), were delivered to the CFUe colonies. The number of colonies in the group without EP was almost 5 times smaller than in the group with EP. Inasmuch as some few colonies are still able to develop, even in absence of added EP, the suggestion is advanced holding that the phenomenon may represent the effect of EP already bound to the group of cells initiating differentiation. Comparisons among all experimental groups indicate that the delivery of sera from turpentine rats to cultures containing added EP, reduced significantly the number of CFUe colonies seen in controls with EP but without inflammatory serum. It is suggested that the present findings could be explained assuming an inhibition of the influence of exogenous EP, subserved by the inflammatory serum, or alternatively that this kind of rat serum induces a partial blockade of EP at receptor sites whose activation is a mandatory step for the adequate and full development of cultured erythrocytes.
format JOUR
author Gutnisky, A.
Gimeno, A.L.
author_facet Gutnisky, A.
Gimeno, A.L.
author_sort Gutnisky, A.
title Action of blood serum from rats with turpentine sterile inflammation on the development of CFU erythrocyte colonies. Possible role of erythropoietin.
title_short Action of blood serum from rats with turpentine sterile inflammation on the development of CFU erythrocyte colonies. Possible role of erythropoietin.
title_full Action of blood serum from rats with turpentine sterile inflammation on the development of CFU erythrocyte colonies. Possible role of erythropoietin.
title_fullStr Action of blood serum from rats with turpentine sterile inflammation on the development of CFU erythrocyte colonies. Possible role of erythropoietin.
title_full_unstemmed Action of blood serum from rats with turpentine sterile inflammation on the development of CFU erythrocyte colonies. Possible role of erythropoietin.
title_sort action of blood serum from rats with turpentine sterile inflammation on the development of cfu erythrocyte colonies. possible role of erythropoietin.
url http://hdl.handle.net/20.500.12110/paper_03266656_v39_n2_p127_Gutnisky
work_keys_str_mv AT gutniskya actionofbloodserumfromratswithturpentinesterileinflammationonthedevelopmentofcfuerythrocytecoloniespossibleroleoferythropoietin
AT gimenoal actionofbloodserumfromratswithturpentinesterileinflammationonthedevelopmentofcfuerythrocytecoloniespossibleroleoferythropoietin
_version_ 1807323888102670336

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