Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole

Metronidazole (MTZ) is a wonderful drug, which is used clinically to treat a wide range of bacterial and protozoal infections. This study aimed to achieve a precise characterization of the cytotoxic and genotoxic activities of MTZ in cultured human lymphocytes at therapeutic concentrations and to ev...

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Autores principales: López Nigro, M.M., Mudry, M.D., Carballo, M.A.
Formato: JOUR
Materias:
Cell death
Cytotoxicity
Genetic toxicology
Genotoxicity
Metronidazole
Short term tests
metronidazole
adult
apoptosis
article
cell death
cell viability
chromosome aberration
chromosome replication
concentration response
controlled study
diploidy
DNA content
DNA structure
drug cytotoxicity
female
genotoxicity
human
human cell
lymphocyte
male
mitosis inhibition
sister chromatid exchange
toxicity testing
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03262383_v29_n8_p1319_LopezNigro
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03262383_v29_n8_p1319_LopezNigro
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spelling todo:paper_03262383_v29_n8_p1319_LopezNigro2023-10-03T15:24:29Z Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole López Nigro, M.M. Mudry, M.D. Carballo, M.A. Cell death Cytotoxicity Genetic toxicology Genotoxicity Metronidazole Short term tests metronidazole adult apoptosis article cell death cell viability chromosome aberration chromosome replication concentration response controlled study diploidy DNA content DNA structure drug cytotoxicity female genotoxicity human human cell lymphocyte male mitosis inhibition sister chromatid exchange toxicity testing Metronidazole (MTZ) is a wonderful drug, which is used clinically to treat a wide range of bacterial and protozoal infections. This study aimed to achieve a precise characterization of the cytotoxic and genotoxic activities of MTZ in cultured human lymphocytes at therapeutic concentrations and to evaluate the possible cell death mechanism associated with it. A significant decrease in Mitotic Index (P < 0.001) as well as an increase in Sister Chromatid Exchange (P < 0.001) and Chromosomal Aberrations (P < 0.001) frequencies with no modifications in Replication Index was observed. DNA extracts of MTZ treated cells resulted in nucleosomal DNA ladder pattern after 48 h of cell treatment and this pattern correlated with a decrease in cellular viability (P < 0.001), morphological evidence of apoptosis and increase in the percentage of nuclei with hypodiploid DNA content (P < 0.001). We concluded that MTZ is genotoxic, cytotoxic and is able to modulate cell death through apoptotic mechanisms in the experimental design employed. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03262383_v29_n8_p1319_LopezNigro
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cell death
Cytotoxicity
Genetic toxicology
Genotoxicity
Metronidazole
Short term tests
metronidazole
adult
apoptosis
article
cell death
cell viability
chromosome aberration
chromosome replication
concentration response
controlled study
diploidy
DNA content
DNA structure
drug cytotoxicity
female
genotoxicity
human
human cell
lymphocyte
male
mitosis inhibition
sister chromatid exchange
toxicity testing
spellingShingle Cell death
Cytotoxicity
Genetic toxicology
Genotoxicity
Metronidazole
Short term tests
metronidazole
adult
apoptosis
article
cell death
cell viability
chromosome aberration
chromosome replication
concentration response
controlled study
diploidy
DNA content
DNA structure
drug cytotoxicity
female
genotoxicity
human
human cell
lymphocyte
male
mitosis inhibition
sister chromatid exchange
toxicity testing
López Nigro, M.M.
Mudry, M.D.
Carballo, M.A.
Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole
topic_facet Cell death
Cytotoxicity
Genetic toxicology
Genotoxicity
Metronidazole
Short term tests
metronidazole
adult
apoptosis
article
cell death
cell viability
chromosome aberration
chromosome replication
concentration response
controlled study
diploidy
DNA content
DNA structure
drug cytotoxicity
female
genotoxicity
human
human cell
lymphocyte
male
mitosis inhibition
sister chromatid exchange
toxicity testing
description Metronidazole (MTZ) is a wonderful drug, which is used clinically to treat a wide range of bacterial and protozoal infections. This study aimed to achieve a precise characterization of the cytotoxic and genotoxic activities of MTZ in cultured human lymphocytes at therapeutic concentrations and to evaluate the possible cell death mechanism associated with it. A significant decrease in Mitotic Index (P < 0.001) as well as an increase in Sister Chromatid Exchange (P < 0.001) and Chromosomal Aberrations (P < 0.001) frequencies with no modifications in Replication Index was observed. DNA extracts of MTZ treated cells resulted in nucleosomal DNA ladder pattern after 48 h of cell treatment and this pattern correlated with a decrease in cellular viability (P < 0.001), morphological evidence of apoptosis and increase in the percentage of nuclei with hypodiploid DNA content (P < 0.001). We concluded that MTZ is genotoxic, cytotoxic and is able to modulate cell death through apoptotic mechanisms in the experimental design employed.
format JOUR
author López Nigro, M.M.
Mudry, M.D.
Carballo, M.A.
author_facet López Nigro, M.M.
Mudry, M.D.
Carballo, M.A.
author_sort López Nigro, M.M.
title Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole
title_short Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole
title_full Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole
title_fullStr Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole
title_full_unstemmed Use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of Metronidazole
title_sort use of short term genotoxicological tests to study cytotoxic, genotoxic and cell death mechanism of metronidazole
url http://hdl.handle.net/20.500.12110/paper_03262383_v29_n8_p1319_LopezNigro
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AT carballoma useofshorttermgenotoxicologicalteststostudycytotoxicgenotoxicandcelldeathmechanismofmetronidazole
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