Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity
This study was conducted to compare different liquid culture media for Paenibacillus larvae growth in order to find the best one to be used in studies on activity of antimicrobial substances, such as essential oils. P. larvae presented poor growth in usual broths such as Mueller-Hinton, commonly emp...
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| Lenguaje: | English |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03257541_v40_n3_p147_Gende |
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todo:paper_03257541_v40_n3_p147_Gende2023-10-03T15:24:17Z Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity Gende, L.B. Eguaras, M.J. Fritz, R. Antimicrobial activity Culture media Paenibacillus larvae antimicrobial activity article bacterial growth bacterial strain bacterium culture controlled study culture medium nonhuman Paenibacillus Culture Media Gram-Positive Endospore-Forming Bacteria Microbial Sensitivity Tests This study was conducted to compare different liquid culture media for Paenibacillus larvae growth in order to find the best one to be used in studies on activity of antimicrobial substances, such as essential oils. P. larvae presented poor growth in usual broths such as Mueller-Hinton, commonly employed in antimicrobial activity assays. Growth in liquid media was evaluated using Paenibacillus larvae strains isolated from hives located in different geographical zones. The MYT medium (Mueller-Hinton broth, yeast extract and thiamine) was selected out of the eight liquid media analyzed, as it proved to be the most adequate due to its higher absorbance at 620 nm. The following mean values were obtained from the four P. larvae strains: 0.227 ± 0.016 for the Cobo strain, 0.279 ± 0.015 for La Plata strain, 0.758 ± 0.020 for Mechongué strain and 0.244 ± 0.0079 for Sierra de los Padres strain, respectively. JOUR English info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v40_n3_p147_Gende |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| language |
English |
| orig_language_str_mv |
English |
| topic |
Antimicrobial activity Culture media Paenibacillus larvae antimicrobial activity article bacterial growth bacterial strain bacterium culture controlled study culture medium nonhuman Paenibacillus Culture Media Gram-Positive Endospore-Forming Bacteria Microbial Sensitivity Tests |
| spellingShingle |
Antimicrobial activity Culture media Paenibacillus larvae antimicrobial activity article bacterial growth bacterial strain bacterium culture controlled study culture medium nonhuman Paenibacillus Culture Media Gram-Positive Endospore-Forming Bacteria Microbial Sensitivity Tests Gende, L.B. Eguaras, M.J. Fritz, R. Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity |
| topic_facet |
Antimicrobial activity Culture media Paenibacillus larvae antimicrobial activity article bacterial growth bacterial strain bacterium culture controlled study culture medium nonhuman Paenibacillus Culture Media Gram-Positive Endospore-Forming Bacteria Microbial Sensitivity Tests |
| description |
This study was conducted to compare different liquid culture media for Paenibacillus larvae growth in order to find the best one to be used in studies on activity of antimicrobial substances, such as essential oils. P. larvae presented poor growth in usual broths such as Mueller-Hinton, commonly employed in antimicrobial activity assays. Growth in liquid media was evaluated using Paenibacillus larvae strains isolated from hives located in different geographical zones. The MYT medium (Mueller-Hinton broth, yeast extract and thiamine) was selected out of the eight liquid media analyzed, as it proved to be the most adequate due to its higher absorbance at 620 nm. The following mean values were obtained from the four P. larvae strains: 0.227 ± 0.016 for the Cobo strain, 0.279 ± 0.015 for La Plata strain, 0.758 ± 0.020 for Mechongué strain and 0.244 ± 0.0079 for Sierra de los Padres strain, respectively. |
| format |
JOUR |
| author |
Gende, L.B. Eguaras, M.J. Fritz, R. |
| author_facet |
Gende, L.B. Eguaras, M.J. Fritz, R. |
| author_sort |
Gende, L.B. |
| title |
Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity |
| title_short |
Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity |
| title_full |
Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity |
| title_fullStr |
Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity |
| title_full_unstemmed |
Evaluation of culture media for Paenibacillus larvae applied to studies of antimicrobial activity |
| title_sort |
evaluation of culture media for paenibacillus larvae applied to studies of antimicrobial activity |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v40_n3_p147_Gende |
| work_keys_str_mv |
AT gendelb evaluationofculturemediaforpaenibacilluslarvaeappliedtostudiesofantimicrobialactivity AT eguarasmj evaluationofculturemediaforpaenibacilluslarvaeappliedtostudiesofantimicrobialactivity AT fritzr evaluationofculturemediaforpaenibacilluslarvaeappliedtostudiesofantimicrobialactivity |
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1782028667166130176 |