Degradation of environmental pollutants by Trametes trogii
The ability of the ligninolytic fungus Trametes trogii to degrade in vitro different xenobiotics (PCBs, PAHs and dyes) was evaluated. Either 200 ppm of a PCB mixture (Aroclor 1150) or 160 ppm of an industrial PAH mixture (10% V/V of PAHs, principal components hexaethylbenzene, naphthalene, 1-methyl...
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todo:paper_03257541_v34_n3_p157_Haglund2023-10-03T15:24:14Z Degradation of environmental pollutants by Trametes trogii Haglund, C. Levin, L. Forchiassin, F. Lopez, M. Viale, A. Degradation Ligninolytic fungi Organopollutants Trametes trogii 1 methylnaphthalene acenaphthylene anthracene anthraquinone aroclor asparagine dye ethylbenzene fluorene glucose laccase manganese peroxidase naphthalene derivative nitrogen organic compound phenanthrene polychlorinated biphenyl derivative polycyclic aromatic hydrocarbon unclassified drug xenobiotic agent article biodegradation concentration (parameters) detoxification enzyme activity enzyme assay fungal metabolism gas liquid chromatography nonhuman pollutant principal component analysis Trametes trametes trogii Aroclors Biodegradation, Environmental Chemical Industry Coloring Agents Fungal Proteins Gas Chromatography-Mass Spectrometry Hydrocarbons, Aromatic Industrial Waste Laccase Oxidoreductases Polychlorinated Biphenyls Polyporales Soil Pollutants Xenobiotics The ability of the ligninolytic fungus Trametes trogii to degrade in vitro different xenobiotics (PCBs, PAHs and dyes) was evaluated. Either 200 ppm of a PCB mixture (Aroclor 1150) or 160 ppm of an industrial PAH mixture (10% V/V of PAHs, principal components hexaethylbenzene, naphthalene, 1-methyl naphthalene, acenaphthylene, anthracene, fluorene and phenanthrene), were added to trophophasic and idiophasic cultures growing in a nitrogen limited mineral medium (glucose/asparagine) and in a complex medium (malt extract/glucose). Gas-liquid chromatography proved that within 7 to 12 d more than 90% of the organopollutants added were removed. The decrease in absorbance at 620 nm demonstrated that cultures of this fungus were able to transform 80% of the dye Anthraquinone-blue (added at a concentration of 50 ppm) in 1.5 h. Enzyme estimations indicated high activity of laccase (up to 0.55 U/mL), as well as lower production of manganese-peroxidase. Laccase activity, detected in all the conditions assayed, could be implicated in the degradation of these organopollutants. Considering the results obtained, T. trogii seems promising for detoxification. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v34_n3_p157_Haglund |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Degradation Ligninolytic fungi Organopollutants Trametes trogii 1 methylnaphthalene acenaphthylene anthracene anthraquinone aroclor asparagine dye ethylbenzene fluorene glucose laccase manganese peroxidase naphthalene derivative nitrogen organic compound phenanthrene polychlorinated biphenyl derivative polycyclic aromatic hydrocarbon unclassified drug xenobiotic agent article biodegradation concentration (parameters) detoxification enzyme activity enzyme assay fungal metabolism gas liquid chromatography nonhuman pollutant principal component analysis Trametes trametes trogii Aroclors Biodegradation, Environmental Chemical Industry Coloring Agents Fungal Proteins Gas Chromatography-Mass Spectrometry Hydrocarbons, Aromatic Industrial Waste Laccase Oxidoreductases Polychlorinated Biphenyls Polyporales Soil Pollutants Xenobiotics |
| spellingShingle |
Degradation Ligninolytic fungi Organopollutants Trametes trogii 1 methylnaphthalene acenaphthylene anthracene anthraquinone aroclor asparagine dye ethylbenzene fluorene glucose laccase manganese peroxidase naphthalene derivative nitrogen organic compound phenanthrene polychlorinated biphenyl derivative polycyclic aromatic hydrocarbon unclassified drug xenobiotic agent article biodegradation concentration (parameters) detoxification enzyme activity enzyme assay fungal metabolism gas liquid chromatography nonhuman pollutant principal component analysis Trametes trametes trogii Aroclors Biodegradation, Environmental Chemical Industry Coloring Agents Fungal Proteins Gas Chromatography-Mass Spectrometry Hydrocarbons, Aromatic Industrial Waste Laccase Oxidoreductases Polychlorinated Biphenyls Polyporales Soil Pollutants Xenobiotics Haglund, C. Levin, L. Forchiassin, F. Lopez, M. Viale, A. Degradation of environmental pollutants by Trametes trogii |
| topic_facet |
Degradation Ligninolytic fungi Organopollutants Trametes trogii 1 methylnaphthalene acenaphthylene anthracene anthraquinone aroclor asparagine dye ethylbenzene fluorene glucose laccase manganese peroxidase naphthalene derivative nitrogen organic compound phenanthrene polychlorinated biphenyl derivative polycyclic aromatic hydrocarbon unclassified drug xenobiotic agent article biodegradation concentration (parameters) detoxification enzyme activity enzyme assay fungal metabolism gas liquid chromatography nonhuman pollutant principal component analysis Trametes trametes trogii Aroclors Biodegradation, Environmental Chemical Industry Coloring Agents Fungal Proteins Gas Chromatography-Mass Spectrometry Hydrocarbons, Aromatic Industrial Waste Laccase Oxidoreductases Polychlorinated Biphenyls Polyporales Soil Pollutants Xenobiotics |
| description |
The ability of the ligninolytic fungus Trametes trogii to degrade in vitro different xenobiotics (PCBs, PAHs and dyes) was evaluated. Either 200 ppm of a PCB mixture (Aroclor 1150) or 160 ppm of an industrial PAH mixture (10% V/V of PAHs, principal components hexaethylbenzene, naphthalene, 1-methyl naphthalene, acenaphthylene, anthracene, fluorene and phenanthrene), were added to trophophasic and idiophasic cultures growing in a nitrogen limited mineral medium (glucose/asparagine) and in a complex medium (malt extract/glucose). Gas-liquid chromatography proved that within 7 to 12 d more than 90% of the organopollutants added were removed. The decrease in absorbance at 620 nm demonstrated that cultures of this fungus were able to transform 80% of the dye Anthraquinone-blue (added at a concentration of 50 ppm) in 1.5 h. Enzyme estimations indicated high activity of laccase (up to 0.55 U/mL), as well as lower production of manganese-peroxidase. Laccase activity, detected in all the conditions assayed, could be implicated in the degradation of these organopollutants. Considering the results obtained, T. trogii seems promising for detoxification. |
| format |
JOUR |
| author |
Haglund, C. Levin, L. Forchiassin, F. Lopez, M. Viale, A. |
| author_facet |
Haglund, C. Levin, L. Forchiassin, F. Lopez, M. Viale, A. |
| author_sort |
Haglund, C. |
| title |
Degradation of environmental pollutants by Trametes trogii |
| title_short |
Degradation of environmental pollutants by Trametes trogii |
| title_full |
Degradation of environmental pollutants by Trametes trogii |
| title_fullStr |
Degradation of environmental pollutants by Trametes trogii |
| title_full_unstemmed |
Degradation of environmental pollutants by Trametes trogii |
| title_sort |
degradation of environmental pollutants by trametes trogii |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v34_n3_p157_Haglund |
| work_keys_str_mv |
AT haglundc degradationofenvironmentalpollutantsbytrametestrogii AT levinl degradationofenvironmentalpollutantsbytrametestrogii AT forchiassinf degradationofenvironmentalpollutantsbytrametestrogii AT lopezm degradationofenvironmentalpollutantsbytrametestrogii AT vialea degradationofenvironmentalpollutantsbytrametestrogii |
| _version_ |
1807318191372763136 |