Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis
The influence of temperature and pH on the activity and stability of the cellulase system (endoglucanase, exoglucanase and cellobiase) was investigated in Nectria catalinensis. Optimal temperature for the activity of the cellulase system ranged from 50 to 55 °C, with an optimum for stability between...
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todo:paper_03257541_v31_n1_p31_Pardo2023-10-03T15:24:09Z Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis Pardo, A.G. Forchiassin, F. Cellulases Fungi Nechia catalinensis beta glucosidase cellulase exoglucanase glucan synthase glucose unclassified drug article ascomycetes enzyme activity enzyme kinetics enzyme stability nectria catalinensis nonhuman pH measurement temperature sensitivity Cellulase Enzyme Stability Hydrogen-Ion Concentration Hypocreales Temperature The influence of temperature and pH on the activity and stability of the cellulase system (endoglucanase, exoglucanase and cellobiase) was investigated in Nectria catalinensis. Optimal temperature for the activity of the cellulase system ranged from 50 to 55 °C, with an optimum for stability between 23 °C and 37 °C after a 72 h incubation period. For the different enzymes, maximal activity was registered between pH 4.2-5,8, with pH 4.8 being close to optimal for all stability studies. The activation energy was 4.97 Kcal mol-1 for endoglucanase, 4.37 Kcal mol-1 for exoglucanase and 13.73 Kcal mol-1 for cellobiase. The K(m) and V(max) values were 1.73 mg CMC ml-1 and 0.45 μmol glucose min-1 mg protein-1 for endoglucanase, 0.22 mg microcrystalline cellulose ml-1 and 57.1 nmol glucose min-1 mg protein-1 for exoglucanase, and 2.95 mM cellobiose and 0.17 μmol glucose min-1 mg protein-1 for cellobiase. Fil:Pardo, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Forchiassin, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v31_n1_p31_Pardo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cellulases Fungi Nechia catalinensis beta glucosidase cellulase exoglucanase glucan synthase glucose unclassified drug article ascomycetes enzyme activity enzyme kinetics enzyme stability nectria catalinensis nonhuman pH measurement temperature sensitivity Cellulase Enzyme Stability Hydrogen-Ion Concentration Hypocreales Temperature |
| spellingShingle |
Cellulases Fungi Nechia catalinensis beta glucosidase cellulase exoglucanase glucan synthase glucose unclassified drug article ascomycetes enzyme activity enzyme kinetics enzyme stability nectria catalinensis nonhuman pH measurement temperature sensitivity Cellulase Enzyme Stability Hydrogen-Ion Concentration Hypocreales Temperature Pardo, A.G. Forchiassin, F. Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis |
| topic_facet |
Cellulases Fungi Nechia catalinensis beta glucosidase cellulase exoglucanase glucan synthase glucose unclassified drug article ascomycetes enzyme activity enzyme kinetics enzyme stability nectria catalinensis nonhuman pH measurement temperature sensitivity Cellulase Enzyme Stability Hydrogen-Ion Concentration Hypocreales Temperature |
| description |
The influence of temperature and pH on the activity and stability of the cellulase system (endoglucanase, exoglucanase and cellobiase) was investigated in Nectria catalinensis. Optimal temperature for the activity of the cellulase system ranged from 50 to 55 °C, with an optimum for stability between 23 °C and 37 °C after a 72 h incubation period. For the different enzymes, maximal activity was registered between pH 4.2-5,8, with pH 4.8 being close to optimal for all stability studies. The activation energy was 4.97 Kcal mol-1 for endoglucanase, 4.37 Kcal mol-1 for exoglucanase and 13.73 Kcal mol-1 for cellobiase. The K(m) and V(max) values were 1.73 mg CMC ml-1 and 0.45 μmol glucose min-1 mg protein-1 for endoglucanase, 0.22 mg microcrystalline cellulose ml-1 and 57.1 nmol glucose min-1 mg protein-1 for exoglucanase, and 2.95 mM cellobiose and 0.17 μmol glucose min-1 mg protein-1 for cellobiase. |
| format |
JOUR |
| author |
Pardo, A.G. Forchiassin, F. |
| author_facet |
Pardo, A.G. Forchiassin, F. |
| author_sort |
Pardo, A.G. |
| title |
Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis |
| title_short |
Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis |
| title_full |
Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis |
| title_fullStr |
Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis |
| title_full_unstemmed |
Influence of temperature and pH on cellulase activity and stability in Nectria catalinensis |
| title_sort |
influence of temperature and ph on cellulase activity and stability in nectria catalinensis |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v31_n1_p31_Pardo |
| work_keys_str_mv |
AT pardoag influenceoftemperatureandphoncellulaseactivityandstabilityinnectriacatalinensis AT forchiassinf influenceoftemperatureandphoncellulaseactivityandstabilityinnectriacatalinensis |
| _version_ |
1807314661019746304 |