Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis
Fusarium oxysporum f.sp. melonis, a pathogen of melon (Cucumis melo L.), was grown in shaken cultures at 26 degrees C in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. The extracellular enzymic complex obtained from these cultures showed lytic activity on plant...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03257541_v27_n4_p191_Alconada |
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todo:paper_03257541_v27_n4_p191_Alconada2023-10-03T15:24:02Z Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis Alconada, T.M. Martínez, M.J. cellulase fungal protein galacturonic acid hexuronic acid polygalacturonase xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article carrot cell wall comparative study cytology drug effect enzymology extracellular space fruit Fusarium hydrolysis potato protoplast Cell Wall Cellulase Daucus carota Extracellular Space Fruit Fungal Proteins Fusarium Hexuronic Acids Hydrolysis Polygalacturonase Protoplasts Solanum tuberosum Xylan Endo-1,3-beta-Xylosidase Xylosidases Fusarium oxysporum f.sp. melonis, a pathogen of melon (Cucumis melo L.), was grown in shaken cultures at 26 degrees C in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. The extracellular enzymic complex obtained from these cultures showed lytic activity on plant tissues, causing maceration of melon fruits, potato tubers and carrot roots. Protoplasts were isolated from melon fruits when the maceration was carried out under appropriate osmotic conditions. This fact suggest a possible relationship between the enzymes produced by Fusarium oxysporum f.sp. melonis and their pathogenicity on melon plants. Fil:Alconada, T.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v27_n4_p191_Alconada |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cellulase fungal protein galacturonic acid hexuronic acid polygalacturonase xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article carrot cell wall comparative study cytology drug effect enzymology extracellular space fruit Fusarium hydrolysis potato protoplast Cell Wall Cellulase Daucus carota Extracellular Space Fruit Fungal Proteins Fusarium Hexuronic Acids Hydrolysis Polygalacturonase Protoplasts Solanum tuberosum Xylan Endo-1,3-beta-Xylosidase Xylosidases |
| spellingShingle |
cellulase fungal protein galacturonic acid hexuronic acid polygalacturonase xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article carrot cell wall comparative study cytology drug effect enzymology extracellular space fruit Fusarium hydrolysis potato protoplast Cell Wall Cellulase Daucus carota Extracellular Space Fruit Fungal Proteins Fusarium Hexuronic Acids Hydrolysis Polygalacturonase Protoplasts Solanum tuberosum Xylan Endo-1,3-beta-Xylosidase Xylosidases Alconada, T.M. Martínez, M.J. Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis |
| topic_facet |
cellulase fungal protein galacturonic acid hexuronic acid polygalacturonase xylan 1,4 beta xylosidase xylan endo 1,3 beta xylosidase article carrot cell wall comparative study cytology drug effect enzymology extracellular space fruit Fusarium hydrolysis potato protoplast Cell Wall Cellulase Daucus carota Extracellular Space Fruit Fungal Proteins Fusarium Hexuronic Acids Hydrolysis Polygalacturonase Protoplasts Solanum tuberosum Xylan Endo-1,3-beta-Xylosidase Xylosidases |
| description |
Fusarium oxysporum f.sp. melonis, a pathogen of melon (Cucumis melo L.), was grown in shaken cultures at 26 degrees C in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. The extracellular enzymic complex obtained from these cultures showed lytic activity on plant tissues, causing maceration of melon fruits, potato tubers and carrot roots. Protoplasts were isolated from melon fruits when the maceration was carried out under appropriate osmotic conditions. This fact suggest a possible relationship between the enzymes produced by Fusarium oxysporum f.sp. melonis and their pathogenicity on melon plants. |
| format |
JOUR |
| author |
Alconada, T.M. Martínez, M.J. |
| author_facet |
Alconada, T.M. Martínez, M.J. |
| author_sort |
Alconada, T.M. |
| title |
Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis |
| title_short |
Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis |
| title_full |
Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis |
| title_fullStr |
Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis |
| title_full_unstemmed |
Isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis |
| title_sort |
isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v27_n4_p191_Alconada |
| work_keys_str_mv |
AT alconadatm isolationofprotoplastsfromvegetabletissuesusingextracellularlyticenzymesfromfusariumoxysporumfspmelonis AT martinezmj isolationofprotoplastsfromvegetabletissuesusingextracellularlyticenzymesfromfusariumoxysporumfspmelonis |
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1807315399446888448 |