Evaluation of the cellulolytic capacity of Ascobolus furfuraceus (Fungi, Ascomycotina)

Growth kinetic variables (dry weight, mycelial protein, extracellular protein and pH evolution) were measured in Ascobolus furfuraceus cultures either with soluble cellulose (CMC) or crystalline cellulose as only carbon sources. When growing on CMC the mycelial protein production reached a maximum a...

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Autores principales:	Mercuri, O.A., Diorio, L.A.
Formato:	JOUR
Materias:	cellulase cellulose fungal protein article Ascomycetes comparative study enzymology growth, development and aging metabolism Ascomycota Cellulase Cellulose Fungal Proteins
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03257541_v27_n3_p130_Mercuri
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03257541_v27_n3_p130_Mercuri
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spelling	todo:paper_03257541_v27_n3_p130_Mercuri2023-10-03T15:24:02Z Evaluation of the cellulolytic capacity of Ascobolus furfuraceus (Fungi, Ascomycotina) Mercuri, O.A. Diorio, L.A. cellulase cellulose fungal protein article Ascomycetes comparative study enzymology growth, development and aging metabolism Ascomycota Cellulase Cellulose Fungal Proteins Growth kinetic variables (dry weight, mycelial protein, extracellular protein and pH evolution) were measured in Ascobolus furfuraceus cultures either with soluble cellulose (CMC) or crystalline cellulose as only carbon sources. When growing on CMC the mycelial protein production reached a maximum at 14 days, while the extracellular protein was maximal at 17 days. On crystalline cellulose more delay was observed (4 and 14 days, respectively). Straight lineal correspondence (r = 0.9883) was observed between the extracellular protein production and enzymatic activity kinetics, showing parallel behavior of these variables. When the biomass and extracellular protein production rates were analysed, the maxima were observed at diverse growth stages. For CMC, the dry weight production rythm precedes the cellulolytic system liberation rythm (maxima at 9 and 14 days, respectively). When crystalline cellulose was the substrate, the maximal rates were inverse, 16th day for biomass production rythm and 12th day for enzyme release rythm. On the basis of such tests and analyses, a model to explain cellulose degration by A. furfuraceus, is proposed. Fil:Mercuri, O.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Diorio, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v27_n3_p130_Mercuri
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	cellulase cellulose fungal protein article Ascomycetes comparative study enzymology growth, development and aging metabolism Ascomycota Cellulase Cellulose Fungal Proteins
spellingShingle	cellulase cellulose fungal protein article Ascomycetes comparative study enzymology growth, development and aging metabolism Ascomycota Cellulase Cellulose Fungal Proteins Mercuri, O.A. Diorio, L.A. Evaluation of the cellulolytic capacity of Ascobolus furfuraceus (Fungi, Ascomycotina)
topic_facet	cellulase cellulose fungal protein article Ascomycetes comparative study enzymology growth, development and aging metabolism Ascomycota Cellulase Cellulose Fungal Proteins
description	Growth kinetic variables (dry weight, mycelial protein, extracellular protein and pH evolution) were measured in Ascobolus furfuraceus cultures either with soluble cellulose (CMC) or crystalline cellulose as only carbon sources. When growing on CMC the mycelial protein production reached a maximum at 14 days, while the extracellular protein was maximal at 17 days. On crystalline cellulose more delay was observed (4 and 14 days, respectively). Straight lineal correspondence (r = 0.9883) was observed between the extracellular protein production and enzymatic activity kinetics, showing parallel behavior of these variables. When the biomass and extracellular protein production rates were analysed, the maxima were observed at diverse growth stages. For CMC, the dry weight production rythm precedes the cellulolytic system liberation rythm (maxima at 9 and 14 days, respectively). When crystalline cellulose was the substrate, the maximal rates were inverse, 16th day for biomass production rythm and 12th day for enzyme release rythm. On the basis of such tests and analyses, a model to explain cellulose degration by A. furfuraceus, is proposed.
format	JOUR
author	Mercuri, O.A. Diorio, L.A.
author_facet	Mercuri, O.A. Diorio, L.A.
author_sort	Mercuri, O.A.
title	Evaluation of the cellulolytic capacity of Ascobolus furfuraceus (Fungi, Ascomycotina)
title_short	Evaluation of the cellulolytic capacity of Ascobolus furfuraceus (Fungi, Ascomycotina)
title_full	Evaluation of the cellulolytic capacity of Ascobolus furfuraceus (Fungi, Ascomycotina)
title_fullStr	Evaluation of the cellulolytic capacity of Ascobolus furfuraceus (Fungi, Ascomycotina)
title_full_unstemmed	Evaluation of the cellulolytic capacity of Ascobolus furfuraceus (Fungi, Ascomycotina)
title_sort	evaluation of the cellulolytic capacity of ascobolus furfuraceus (fungi, ascomycotina)
url	http://hdl.handle.net/20.500.12110/paper_03257541_v27_n3_p130_Mercuri
work_keys_str_mv	AT mercurioa evaluationofthecellulolyticcapacityofascobolusfurfuraceusfungiascomycotina AT dioriola evaluationofthecellulolyticcapacityofascobolusfurfuraceusfungiascomycotina
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Evaluation of the cellulolytic capacity of Ascobolus furfuraceus (Fungi, Ascomycotina)

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