Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions
The effects of different experimental conditions on the antiviral activity of the sulphated xylomannan F6, isolated from the red seaweed Nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) were studied. The compound was equally effective against seve...
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todo:paper_03257541_v27_n2_p91_Pujol2023-10-03T15:24:01Z Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions Pujol, C.A. Coto, C.E. Damonte, E.B. antivirus agent mannan animal article cell culture cell line cell survival Cercopithecus chemistry drug effect drug screening hamster Herpes simplex virus human isolation and purification kidney pathology physiology seaweed skin tumor squamous cell carcinoma Vero cell virus culture virus replication Animals Antiviral Agents Carcinoma, Squamous Cell Cell Line Cell Survival Cercopithecus aethiops Cricetinae Drug Evaluation, Preclinical Humans Kidney Mannans Plaque Assay Seaweed Simplexvirus Skin Neoplasms Tumor Cells, Cultured Vero Cells Virus Replication The effects of different experimental conditions on the antiviral activity of the sulphated xylomannan F6, isolated from the red seaweed Nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) were studied. The compound was equally effective against several strains of HSV-1 (F, KOS, B-2006 and Field) and HSV-2 (G) in different cell lines (Vero, HEp-2 and BHK-21). Furthermore, the antiviral activity of F6 was independent of the method employed to determine the 50% inhibitory concentration (IC50) (virus yield or plaque reduction tests). The value of the IC50 against HSV-1 was not significantly affected by the initial virus concentration since a variation in the multiplicity of infection from 0.0001 to 0.1 UFP/cell only increased twofold the IC50 from 0.61 to 1.35 micrograms/ml, respectively. The potent inhibitory effect of F6 against high doses of HSV-1 represents a significant advantage for this sulphated polysaccharide with respect to other compounds only active against low viral inocula. Fil:Pujol, C.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v27_n2_p91_Pujol |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
antivirus agent mannan animal article cell culture cell line cell survival Cercopithecus chemistry drug effect drug screening hamster Herpes simplex virus human isolation and purification kidney pathology physiology seaweed skin tumor squamous cell carcinoma Vero cell virus culture virus replication Animals Antiviral Agents Carcinoma, Squamous Cell Cell Line Cell Survival Cercopithecus aethiops Cricetinae Drug Evaluation, Preclinical Humans Kidney Mannans Plaque Assay Seaweed Simplexvirus Skin Neoplasms Tumor Cells, Cultured Vero Cells Virus Replication |
| spellingShingle |
antivirus agent mannan animal article cell culture cell line cell survival Cercopithecus chemistry drug effect drug screening hamster Herpes simplex virus human isolation and purification kidney pathology physiology seaweed skin tumor squamous cell carcinoma Vero cell virus culture virus replication Animals Antiviral Agents Carcinoma, Squamous Cell Cell Line Cell Survival Cercopithecus aethiops Cricetinae Drug Evaluation, Preclinical Humans Kidney Mannans Plaque Assay Seaweed Simplexvirus Skin Neoplasms Tumor Cells, Cultured Vero Cells Virus Replication Pujol, C.A. Coto, C.E. Damonte, E.B. Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions |
| topic_facet |
antivirus agent mannan animal article cell culture cell line cell survival Cercopithecus chemistry drug effect drug screening hamster Herpes simplex virus human isolation and purification kidney pathology physiology seaweed skin tumor squamous cell carcinoma Vero cell virus culture virus replication Animals Antiviral Agents Carcinoma, Squamous Cell Cell Line Cell Survival Cercopithecus aethiops Cricetinae Drug Evaluation, Preclinical Humans Kidney Mannans Plaque Assay Seaweed Simplexvirus Skin Neoplasms Tumor Cells, Cultured Vero Cells Virus Replication |
| description |
The effects of different experimental conditions on the antiviral activity of the sulphated xylomannan F6, isolated from the red seaweed Nothogenia fastigiata, against the replication of herpes simplex virus type 1 and 2 (HSV-1 and HSV-2) were studied. The compound was equally effective against several strains of HSV-1 (F, KOS, B-2006 and Field) and HSV-2 (G) in different cell lines (Vero, HEp-2 and BHK-21). Furthermore, the antiviral activity of F6 was independent of the method employed to determine the 50% inhibitory concentration (IC50) (virus yield or plaque reduction tests). The value of the IC50 against HSV-1 was not significantly affected by the initial virus concentration since a variation in the multiplicity of infection from 0.0001 to 0.1 UFP/cell only increased twofold the IC50 from 0.61 to 1.35 micrograms/ml, respectively. The potent inhibitory effect of F6 against high doses of HSV-1 represents a significant advantage for this sulphated polysaccharide with respect to other compounds only active against low viral inocula. |
| format |
JOUR |
| author |
Pujol, C.A. Coto, C.E. Damonte, E.B. |
| author_facet |
Pujol, C.A. Coto, C.E. Damonte, E.B. |
| author_sort |
Pujol, C.A. |
| title |
Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions |
| title_short |
Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions |
| title_full |
Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions |
| title_fullStr |
Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions |
| title_full_unstemmed |
Determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions |
| title_sort |
determination of the antiviral activity of a naturally occurring sulfated xylomannan under various experimental conditions |
| url |
http://hdl.handle.net/20.500.12110/paper_03257541_v27_n2_p91_Pujol |
| work_keys_str_mv |
AT pujolca determinationoftheantiviralactivityofanaturallyoccurringsulfatedxylomannanundervariousexperimentalconditions AT cotoce determinationoftheantiviralactivityofanaturallyoccurringsulfatedxylomannanundervariousexperimentalconditions AT damonteeb determinationoftheantiviralactivityofanaturallyoccurringsulfatedxylomannanundervariousexperimentalconditions |
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1807316555776655360 |