Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)

Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population.

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Detalles Bibliográficos
Autores principales:	Floccari, M.E., Tiscornia, G., Palmada, F., Méndez, B.S.
Formato:	JOUR
Materias:	hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester article Bacillus megaterium bacterial spore density gradient centrifugation genetics isolation and purification metabolism Centrifugation, Density Gradient Hydroxybutyrates Polyesters Spores, Bacterial
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03257541_v23_n1_p26_Floccari
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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