Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)

Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population.

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Detalles Bibliográficos
Autores principales:	Floccari, M.E., Tiscornia, G., Palmada, F., Méndez, B.S.
Formato:	JOUR
Materias:	hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester article Bacillus megaterium bacterial spore density gradient centrifugation genetics isolation and purification metabolism Centrifugation, Density Gradient Hydroxybutyrates Polyesters Spores, Bacterial
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_03257541_v23_n1_p26_Floccari
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_03257541_v23_n1_p26_Floccari
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spelling	todo:paper_03257541_v23_n1_p26_Floccari2023-10-03T15:23:57Z Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB) Floccari, M.E. Tiscornia, G. Palmada, F. Méndez, B.S. hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester article Bacillus megaterium bacterial spore density gradient centrifugation genetics isolation and purification metabolism Bacillus megaterium Centrifugation, Density Gradient Hydroxybutyrates Polyesters Spores, Bacterial Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population. Fil:Floccari, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tiscornia, G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v23_n1_p26_Floccari
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester article Bacillus megaterium bacterial spore density gradient centrifugation genetics isolation and purification metabolism Bacillus megaterium Centrifugation, Density Gradient Hydroxybutyrates Polyesters Spores, Bacterial
spellingShingle	hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester article Bacillus megaterium bacterial spore density gradient centrifugation genetics isolation and purification metabolism Bacillus megaterium Centrifugation, Density Gradient Hydroxybutyrates Polyesters Spores, Bacterial Floccari, M.E. Tiscornia, G. Palmada, F. Méndez, B.S. Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)
topic_facet	hydroxybutyric acid poly(3 hydroxybutyric acid) poly-beta-hydroxybutyrate polyester article Bacillus megaterium bacterial spore density gradient centrifugation genetics isolation and purification metabolism Bacillus megaterium Centrifugation, Density Gradient Hydroxybutyrates Polyesters Spores, Bacterial
description	Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population.
format	JOUR
author	Floccari, M.E. Tiscornia, G. Palmada, F. Méndez, B.S.
author_facet	Floccari, M.E. Tiscornia, G. Palmada, F. Méndez, B.S.
author_sort	Floccari, M.E.
title	Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)
title_short	Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)
title_full	Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)
title_fullStr	Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)
title_full_unstemmed	Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)
title_sort	enrichment for mutants of bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (phb)
url	http://hdl.handle.net/20.500.12110/paper_03257541_v23_n1_p26_Floccari
work_keys_str_mv	AT floccarime enrichmentformutantsofbacillusmegateriumdeficientinthesynthesisofpolybetahydroxybutyratephb AT tiscorniag enrichmentformutantsofbacillusmegateriumdeficientinthesynthesisofpolybetahydroxybutyratephb AT palmadaf enrichmentformutantsofbacillusmegateriumdeficientinthesynthesisofpolybetahydroxybutyratephb AT mendezbs enrichmentformutantsofbacillusmegateriumdeficientinthesynthesisofpolybetahydroxybutyratephb
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Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)

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