Enrichment for mutants of Bacillus megaterium deficient in the synthesis of poly-beta-hydroxybutyrate (PHB)

Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population.

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Detalles Bibliográficos
Autores principales: Floccari, M.E., Tiscornia, G., Palmada, F., Méndez, B.S.
Formato: JOUR
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03257541_v23_n1_p26_Floccari
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Sumario:Centrifugation through sucrose gradients was adapted to separate spore-forming cells of B. megaterium deficient in poly-beta-hydroxybutyrate synthesis from wild type cells. The conditions described allowed the detection of mutant clones screening a low percentage of the mutagenized population.