Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L.

Crude extracts from fresh green leaves of Melia azedarach L contain an antiviral factor (FAV) able to inhibit the replication of several animal viruses, e.g. Polio, VSV, HSV, FMDV, Sindbis, Junín, Pichinde and Tacaribe in Vero or BHK-21 cells. Crude preparations were subjected to different steps of...

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Autores principales: Andrei, G.M., Coto, C.E., de Torres, R.A.
Formato: JOUR
Materias:
antivirus agent
plant extract
Arenavirus
Argentina
article
cell division
cell survival
drug effect
Herpes virus
medicinal plant
physiology
Poliomyelitis virus
Sindbis virus
tree
virus replication
Antiviral Agents
Arenaviruses, New World
Cell Division
Cell Survival
Herpesviridae
Plant Extracts
Plants, Medicinal
Poliovirus
Sindbis Virus
Trees
Virus Replication
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03257541_v17_n4_p187_Andrei
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_03257541_v17_n4_p187_Andrei
record_format dspace
spelling todo:paper_03257541_v17_n4_p187_Andrei2023-10-03T15:23:53Z Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. Andrei, G.M. Coto, C.E. de Torres, R.A. antivirus agent plant extract Arenavirus Argentina article cell division cell survival drug effect Herpes virus medicinal plant physiology Poliomyelitis virus Sindbis virus tree virus replication Antiviral Agents Arenaviruses, New World Argentina Cell Division Cell Survival Herpesviridae Plant Extracts Plants, Medicinal Poliovirus Sindbis Virus Trees Virus Replication Crude extracts from fresh green leaves of Melia azedarach L contain an antiviral factor (FAV) able to inhibit the replication of several animal viruses, e.g. Polio, VSV, HSV, FMDV, Sindbis, Junín, Pichinde and Tacaribe in Vero or BHK-21 cells. Crude preparations were subjected to different steps of purification like chromatography on Sephadex G-100 and DEAE-Sephadex. The antiviral activity of G-100 and DEAE fractions was fully conserved, whereas contaminating proteins were lost. Two types of cytotoxicity tests were performed with the different fractions. Two-fold serial dilutions of each of them were added to preformed monolayers of Vero or BHK-21 cells and cellular viability was tested. While crude extracts were toxic at low dilutions (less than or equal to 1:10), G-100 and DEAE fractions were not. The other cytotoxicity assay consisted in seeding the cells in the presence of different concentrations of each fraction. G-100 fraction affected cell growth at low dilutions (less than or equal to 1:5), while DEAE fraction did not. It should be remarked that the purification procedure rendered a partial purified DEAE fraction with an increased specific activity (antiviral activity/mg of protein). It is concluded that an antiviral factor devoid of toxicity exists in M. azedarach L extracts, which exhibited a broad spectrum of antiviral activity. Fil:Andrei, G.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03257541_v17_n4_p187_Andrei
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic antivirus agent
plant extract
Arenavirus
Argentina
article
cell division
cell survival
drug effect
Herpes virus
medicinal plant
physiology
Poliomyelitis virus
Sindbis virus
tree
virus replication
Antiviral Agents
Arenaviruses, New World
Argentina
Cell Division
Cell Survival
Herpesviridae
Plant Extracts
Plants, Medicinal
Poliovirus
Sindbis Virus
Trees
Virus Replication
spellingShingle antivirus agent
plant extract
Arenavirus
Argentina
article
cell division
cell survival
drug effect
Herpes virus
medicinal plant
physiology
Poliomyelitis virus
Sindbis virus
tree
virus replication
Antiviral Agents
Arenaviruses, New World
Argentina
Cell Division
Cell Survival
Herpesviridae
Plant Extracts
Plants, Medicinal
Poliovirus
Sindbis Virus
Trees
Virus Replication
Andrei, G.M.
Coto, C.E.
de Torres, R.A.
Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L.
topic_facet antivirus agent
plant extract
Arenavirus
Argentina
article
cell division
cell survival
drug effect
Herpes virus
medicinal plant
physiology
Poliomyelitis virus
Sindbis virus
tree
virus replication
Antiviral Agents
Arenaviruses, New World
Argentina
Cell Division
Cell Survival
Herpesviridae
Plant Extracts
Plants, Medicinal
Poliovirus
Sindbis Virus
Trees
Virus Replication
description Crude extracts from fresh green leaves of Melia azedarach L contain an antiviral factor (FAV) able to inhibit the replication of several animal viruses, e.g. Polio, VSV, HSV, FMDV, Sindbis, Junín, Pichinde and Tacaribe in Vero or BHK-21 cells. Crude preparations were subjected to different steps of purification like chromatography on Sephadex G-100 and DEAE-Sephadex. The antiviral activity of G-100 and DEAE fractions was fully conserved, whereas contaminating proteins were lost. Two types of cytotoxicity tests were performed with the different fractions. Two-fold serial dilutions of each of them were added to preformed monolayers of Vero or BHK-21 cells and cellular viability was tested. While crude extracts were toxic at low dilutions (less than or equal to 1:10), G-100 and DEAE fractions were not. The other cytotoxicity assay consisted in seeding the cells in the presence of different concentrations of each fraction. G-100 fraction affected cell growth at low dilutions (less than or equal to 1:5), while DEAE fraction did not. It should be remarked that the purification procedure rendered a partial purified DEAE fraction with an increased specific activity (antiviral activity/mg of protein). It is concluded that an antiviral factor devoid of toxicity exists in M. azedarach L extracts, which exhibited a broad spectrum of antiviral activity.
format JOUR
author Andrei, G.M.
Coto, C.E.
de Torres, R.A.
author_facet Andrei, G.M.
Coto, C.E.
de Torres, R.A.
author_sort Andrei, G.M.
title Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L.
title_short Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L.
title_full Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L.
title_fullStr Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L.
title_full_unstemmed Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L.
title_sort assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of melia azedarach l.
url http://hdl.handle.net/20.500.12110/paper_03257541_v17_n4_p187_Andrei
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