Cotinine detection and tobacco smoke exposure

Tobacco is one of the major preventable causes of morbidity and premature mortality in the world. A simple, rapid method for determination of cotinine, major metabolite of nicotine in urine, is described. The assay involves a fast liquid-liquid extraction with chloroform as a solvent in alkaline env...

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Autores principales: Vacchino, M.N., Velurtas, S.M., Salinas, G.P., Garcialoredo, H.H.
Formato: JOUR
Lenguaje:Spanish
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_03252957_v40_n2_p181_Vacchino
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spelling todo:paper_03252957_v40_n2_p181_Vacchino2023-10-03T15:23:39Z Cotinine detection and tobacco smoke exposure Vacchino, M.N. Velurtas, S.M. Salinas, G.P. Garcialoredo, H.H. Cotinine Environmental tobacco smoke Epidemiological studies Second hand smoker Nicotiana tabacum Tobacco is one of the major preventable causes of morbidity and premature mortality in the world. A simple, rapid method for determination of cotinine, major metabolite of nicotine in urine, is described. The assay involves a fast liquid-liquid extraction with chloroform as a solvent in alkaline environment. The extract was dried at 37°C and the residue was dissolved in 100 μL of standard internal of pyridine in methanol solution, and an aliquot was analyzed by gas-liquid chromatograph equipped with a flame -ionization detector. This technique was performed to test urine of a sample of smokers and non-smokers who were previously asked about their smoking habit. The limit of detection was 3.1 ng/mL, and using two calibration curves, a linearity range from 3 a 5.000 ng/mL was covered. The median and quartiles (Q) of cotinine in smokers were 255.4 ng/mL, 15.9 ng/mL (Q1) - 1050.2 ng/mL (Q3) ng/mL and in non-smokers 15.8 ng/mL, 4.1 ng/mL (Q1) - 38.4 ng/mL (Q3). Wilcoxon sumrank: z = 4.865 p < 0.01. There was a relationship between cotinine concentration in urine and number of smoked cigarettes. Spearman's rho = 0.5672 (p < 0.001). This technique could identify smokers, second hand smokers and non-smokers using cut-off of 10-15 ng/mL of cotinine in urine, usually adopted in epidemiological studies. JOUR Spanish info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03252957_v40_n2_p181_Vacchino
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
language Spanish
orig_language_str_mv Spanish
topic Cotinine
Environmental tobacco smoke
Epidemiological studies
Second hand smoker
Nicotiana tabacum
spellingShingle Cotinine
Environmental tobacco smoke
Epidemiological studies
Second hand smoker
Nicotiana tabacum
Vacchino, M.N.
Velurtas, S.M.
Salinas, G.P.
Garcialoredo, H.H.
Cotinine detection and tobacco smoke exposure
topic_facet Cotinine
Environmental tobacco smoke
Epidemiological studies
Second hand smoker
Nicotiana tabacum
description Tobacco is one of the major preventable causes of morbidity and premature mortality in the world. A simple, rapid method for determination of cotinine, major metabolite of nicotine in urine, is described. The assay involves a fast liquid-liquid extraction with chloroform as a solvent in alkaline environment. The extract was dried at 37°C and the residue was dissolved in 100 μL of standard internal of pyridine in methanol solution, and an aliquot was analyzed by gas-liquid chromatograph equipped with a flame -ionization detector. This technique was performed to test urine of a sample of smokers and non-smokers who were previously asked about their smoking habit. The limit of detection was 3.1 ng/mL, and using two calibration curves, a linearity range from 3 a 5.000 ng/mL was covered. The median and quartiles (Q) of cotinine in smokers were 255.4 ng/mL, 15.9 ng/mL (Q1) - 1050.2 ng/mL (Q3) ng/mL and in non-smokers 15.8 ng/mL, 4.1 ng/mL (Q1) - 38.4 ng/mL (Q3). Wilcoxon sumrank: z = 4.865 p < 0.01. There was a relationship between cotinine concentration in urine and number of smoked cigarettes. Spearman's rho = 0.5672 (p < 0.001). This technique could identify smokers, second hand smokers and non-smokers using cut-off of 10-15 ng/mL of cotinine in urine, usually adopted in epidemiological studies.
format JOUR
author Vacchino, M.N.
Velurtas, S.M.
Salinas, G.P.
Garcialoredo, H.H.
author_facet Vacchino, M.N.
Velurtas, S.M.
Salinas, G.P.
Garcialoredo, H.H.
author_sort Vacchino, M.N.
title Cotinine detection and tobacco smoke exposure
title_short Cotinine detection and tobacco smoke exposure
title_full Cotinine detection and tobacco smoke exposure
title_fullStr Cotinine detection and tobacco smoke exposure
title_full_unstemmed Cotinine detection and tobacco smoke exposure
title_sort cotinine detection and tobacco smoke exposure
url http://hdl.handle.net/20.500.12110/paper_03252957_v40_n2_p181_Vacchino
work_keys_str_mv AT vacchinomn cotininedetectionandtobaccosmokeexposure
AT velurtassm cotininedetectionandtobaccosmokeexposure
AT salinasgp cotininedetectionandtobaccosmokeexposure
AT garcialoredohh cotininedetectionandtobaccosmokeexposure
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