Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa
Wild-type Neurospora crassa grown in minimal medium was exposed to -difluormethyl ornithine (DFMO), a specific inhibitor of ornithine-decarboxylase (ODC-ase) activity. Protein-synthesis rates impaired by DFMO were restored by the addition of spermidine. The pattern on SDS-acrylamide gels displayed t...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_03091651_v14_n1_p69_Grotewold |
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todo:paper_03091651_v14_n1_p69_Grotewold2023-10-03T15:23:00Z Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa Grotewold, E. Aisemberg, G.O. Taccioli, G.E. Judewicz, N.D. gene activation Neurospora crassa ODC-ase inhibition polyamine deficiency ornithine decarboxylase polyamine article fungus gene expression neurospora crassa nonhuman priority journal Eflornithine Enzyme Inhibitors Gene Expression Regulation, Fungal Neurospora Neurospora crassa Ornithine Decarboxylase Polyamines Spermidine Fungi Neurospora crassa Wild-type Neurospora crassa grown in minimal medium was exposed to -difluormethyl ornithine (DFMO), a specific inhibitor of ornithine-decarboxylase (ODC-ase) activity. Protein-synthesis rates impaired by DFMO were restored by the addition of spermidine. The pattern on SDS-acrylamide gels displayed three newly synthesized polypeptides, p27, p31 and p99 after DFMO action in the absence of exogenous polyamine. The ODC-ase mutant (spe-1) grown in spermidine-supplemented medium did not show an induced polypeptide pattern. The lack of ODC-ase activity promotes the expression of p27- and p31-coding genes in both strains but transcription of p31 gene is shut-off after spermidine addition. Both transcripts are also accumulated after exposure to low cycloheximide doses or nutrient starvation. Another cycloheximide-inducible gene coding for p70 is also expressed under DFMO-treatment. © 1990. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_03091651_v14_n1_p69_Grotewold |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
gene activation Neurospora crassa ODC-ase inhibition polyamine deficiency ornithine decarboxylase polyamine article fungus gene expression neurospora crassa nonhuman priority journal Eflornithine Enzyme Inhibitors Gene Expression Regulation, Fungal Neurospora Neurospora crassa Ornithine Decarboxylase Polyamines Spermidine Fungi Neurospora crassa |
| spellingShingle |
gene activation Neurospora crassa ODC-ase inhibition polyamine deficiency ornithine decarboxylase polyamine article fungus gene expression neurospora crassa nonhuman priority journal Eflornithine Enzyme Inhibitors Gene Expression Regulation, Fungal Neurospora Neurospora crassa Ornithine Decarboxylase Polyamines Spermidine Fungi Neurospora crassa Grotewold, E. Aisemberg, G.O. Taccioli, G.E. Judewicz, N.D. Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa |
| topic_facet |
gene activation Neurospora crassa ODC-ase inhibition polyamine deficiency ornithine decarboxylase polyamine article fungus gene expression neurospora crassa nonhuman priority journal Eflornithine Enzyme Inhibitors Gene Expression Regulation, Fungal Neurospora Neurospora crassa Ornithine Decarboxylase Polyamines Spermidine Fungi Neurospora crassa |
| description |
Wild-type Neurospora crassa grown in minimal medium was exposed to -difluormethyl ornithine (DFMO), a specific inhibitor of ornithine-decarboxylase (ODC-ase) activity. Protein-synthesis rates impaired by DFMO were restored by the addition of spermidine. The pattern on SDS-acrylamide gels displayed three newly synthesized polypeptides, p27, p31 and p99 after DFMO action in the absence of exogenous polyamine. The ODC-ase mutant (spe-1) grown in spermidine-supplemented medium did not show an induced polypeptide pattern. The lack of ODC-ase activity promotes the expression of p27- and p31-coding genes in both strains but transcription of p31 gene is shut-off after spermidine addition. Both transcripts are also accumulated after exposure to low cycloheximide doses or nutrient starvation. Another cycloheximide-inducible gene coding for p70 is also expressed under DFMO-treatment. © 1990. |
| format |
JOUR |
| author |
Grotewold, E. Aisemberg, G.O. Taccioli, G.E. Judewicz, N.D. |
| author_facet |
Grotewold, E. Aisemberg, G.O. Taccioli, G.E. Judewicz, N.D. |
| author_sort |
Grotewold, E. |
| title |
Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa |
| title_short |
Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa |
| title_full |
Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa |
| title_fullStr |
Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa |
| title_full_unstemmed |
Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa |
| title_sort |
genes responsive to the alteration of polyamine biosynthesis in neurospora crassa |
| url |
http://hdl.handle.net/20.500.12110/paper_03091651_v14_n1_p69_Grotewold |
| work_keys_str_mv |
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| _version_ |
1807316422492160000 |